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The map label for this gene is ilvD [H]

Identifier: 29143716

GI number: 29143716

Start: 3495404

End: 3497254

Strand: Reverse

Name: ilvD [H]

Synonym: t3394

Alternate gene names: 29143716

Gene position: 3497254-3495404 (Counterclockwise)

Preceding gene: 29143717

Following gene: 29143715

Centisome position: 72.98

GC content: 57.64

Gene sequence:

>1851_bases
ATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCCCGCGCGCTGTGGCGCGCCACCGGAAT
GACCGACAGTGATTTTGGCAAACCGATTATCACCGTGGTGAACTCATTCACTCAGTTTGTGCCGGGTCACGTTCATCTGC
GCGATCTCGGTAAGCTGGTCGCCGAACAGATTGAAGCTTCCGGCGGTGTGGCGAAAGAGTTCAACACTATTGCCGTGGAT
GACGGGATCGCCATGGGGCACGGGGGTATGCTCTATTCACTGCCGTCGCGCGAGCTGATCGCCGACTCCGTGGAGTACAT
GGTGAACGCTCACTGCGCTGACGCGATGGTGTGTATCTCCAACTGCGACAAAATCACCCCAGGGATGCTCATGGCCTCGC
TGCGCCTGAATATTCCGGTGATCTTTGTCTCCGGCGGCCCGATGGAAGCCGGGAAAACCAAACTGTCCGATCAGATCATC
AAGCTCGATCTGGTTGATGCCATGATTCAGGGAGCGGACCCGAAAGTCTCTGACGATCAAAGTAACCAGGTTGAACGCTC
CGCCTGTCCAACCTGCGGCTCCTGCTCCGGCATGTTTACCGCTAACTCCATGAATTGCCTGATCGAAGCGCTGGGCCTGT
CGCAGCCGGGCAACGGCTCGCTGCTGGCGACCCATGCTGACCGGAAGCAGTTGTTCCTCAATGCTGGTAAGCGGATTGTT
GAACTGACTAAACGCTATTACGAGCAGGATGACGAAAGCGCACTGCCGCGTAACATCGCCAACAAGGCGGCGTTTGAGAA
TGCGATGACGCTGGATATTGCGATGGGCGGTTCGACCAATACCGTTCTGCATCTACTGGCGGCGGCGCAGGAAGCGGAAA
TCGACTTCACCATGAGTGATATCGACAAGCTGTCCCGCAAGGTGCCGCAGCTGTGTAAAGTGGCGCCAAGTACCCAGAAA
TACCATATGGAAGATGTTCACCGTGCCGGCGGTGTGCTGGGTATTTTAGGCGAGCTGGATCGCGCCGGGCTGCTGAACCG
CAACGTGAAAAACGTATTAGGCCTGACGCTGCCGCAAACGCTGGAACAGTACGACATCACGGTTACGCAGGACGAAGCGG
TTAAAAAAATGTTCCGTGCTGGCCCTGCCGGTATCCGTACTACCCAGGCGTTCTCTCAGGATTGTCGCTGGGATTCGCTG
GATGACGACCGCGCAGCGGGTTGCATCCGCTCGCTGGAATATGCCTATAGCAAAGACGGCGGTCTGGCGGTGCTGTATGG
CAACTTCGCCGAAAACGGCTGCATCGTAAAAACCGCGGGCGTGGATGACAGCATCCTTAAATTTACCGGTCCTGTCAAAG
TTTACGAAAGCCAGGACGATGCGGTAGAGGCGATTCTCGGCGGCAAAGTAGTGGAAGGCGATGTGGTCGTAATCCGCTAC
GAAGGGCCGAAAGGCGGGCCGGGAATGCAGGAAATGCTCTATCCGACCAGTTTCCTGAAGTCGATGGGGCTGGGCAAAGC
CTGCGCGCTCATCACCGATGGGCGTTTCTCCGGCGGTACTTCGGGTCTTTCCATCGGCCACGTCTCGCCGGAAGCGGCCA
GCGGCGGCACTATTGCGCTGATTGAAGATGGCGACACCATTGCGATTGATATCCCGAACCGCAGCATTCAGTTGCAGTTG
AGCGAGGCTGAAATCGCCGCACGCCGCGAGGCGCAGGAAGCTCGCGGCGATAAAGCCTGGACGCCGAAAAATCGTCAGCG
TCAGGTTTCGTTTGCCCTGCGTGCCTACGCCAGCCTGGCGACCAGCGCCGATAAAGGCGCGGTGCGCGATAAATCGAAAC
TGGGAGGTTGA

Upstream 100 bases:

>100_bases
GCGAATCCCCAGGAGCTTACTGTAGTAAGTGACTGGAGTGAGGCAGCCAACGCACCTGTGGCGTGAAGTATGAAGGGAAA
AGATGGGGAGTAAATAAAGC

Downstream 100 bases:

>100_bases
TGATGGCGGAATCTCAACCTCTGTCAGTCGCGCCGGAAGGGGCGGAGTATCTGCGCGCGGTGCTACGCGCGCCAGTATAC
GAAGCGGCGCAGGTGACGCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MPKYRSATTTHGRNMAGARALWRATGMTDSDFGKPIITVVNSFTQFVPGHVHLRDLGKLVAEQIEASGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
KLDLVDAMIQGADPKVSDDQSNQVERSACPTCGSCSGMFTANSMNCLIEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
ELTKRYYEQDDESALPRNIANKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQK
YHMEDVHRAGGVLGILGELDRAGLLNRNVKNVLGLTLPQTLEQYDITVTQDEAVKKMFRAGPAGIRTTQAFSQDCRWDSL
DDDRAAGCIRSLEYAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPVKVYESQDDAVEAILGGKVVEGDVVVIRY
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGTIALIEDGDTIAIDIPNRSIQLQL
SEAEIAARREAQEARGDKAWTPKNRQRQVSFALRAYASLATSADKGAVRDKSKLGG

Sequences:

>Translated_616_residues
MPKYRSATTTHGRNMAGARALWRATGMTDSDFGKPIITVVNSFTQFVPGHVHLRDLGKLVAEQIEASGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
KLDLVDAMIQGADPKVSDDQSNQVERSACPTCGSCSGMFTANSMNCLIEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
ELTKRYYEQDDESALPRNIANKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQK
YHMEDVHRAGGVLGILGELDRAGLLNRNVKNVLGLTLPQTLEQYDITVTQDEAVKKMFRAGPAGIRTTQAFSQDCRWDSL
DDDRAAGCIRSLEYAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPVKVYESQDDAVEAILGGKVVEGDVVVIRY
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGTIALIEDGDTIAIDIPNRSIQLQL
SEAEIAARREAQEARGDKAWTPKNRQRQVSFALRAYASLATSADKGAVRDKSKLGG
>Mature_615_residues
PKYRSATTTHGRNMAGARALWRATGMTDSDFGKPIITVVNSFTQFVPGHVHLRDLGKLVAEQIEASGGVAKEFNTIAVDD
GIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIK
LDLVDAMIQGADPKVSDDQSNQVERSACPTCGSCSGMFTANSMNCLIEALGLSQPGNGSLLATHADRKQLFLNAGKRIVE
LTKRYYEQDDESALPRNIANKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKY
HMEDVHRAGGVLGILGELDRAGLLNRNVKNVLGLTLPQTLEQYDITVTQDEAVKKMFRAGPAGIRTTQAFSQDCRWDSLD
DDRAAGCIRSLEYAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPVKVYESQDDAVEAILGGKVVEGDVVVIRYE
GPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGTIALIEDGDTIAIDIPNRSIQLQLS
EAEIAARREAQEARGDKAWTPKNRQRQVSFALRAYASLATSADKGAVRDKSKLGG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=616, Percent_Identity=95.2922077922078, Blast_Score=1222, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=539, Percent_Identity=29.3135435992579, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1786464, Length=523, Percent_Identity=30.2103250478011, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI2367371, Length=542, Percent_Identity=28.5977859778598, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=615, Percent_Identity=36.5853658536585, Blast_Score=335, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65801; Mature: 65669

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYRSATTTHGRNMAGARALWRATGMTDSDFGKPIITVVNSFTQFVPGHVHLRDLGKLV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AEQIEASGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHCCCCCCCCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDDQ
CCCCCCHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SNQVERSACPTCGSCSGMFTANSMNCLIEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
CCCHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHH
ELTKRYYEQDDESALPRNIANKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD
HHHHHHHCCCCCHHCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEHHH
IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVLGILGELDRAGLLNRNVKNVLGLTLPQT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHHHHCCCCHHH
LEQYDITVTQDEAVKKMFRAGPAGIRTTQAFSQDCRWDSLDDDRAAGCIRSLEYAYSKDG
HHHHCCEEEHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
GLAVLYGNFAENGCIVKTAGVDDSILKFTGPVKVYESQDDAVEAILGGKVVEGDVVVIRY
CEEEEECCCCCCCCEEEECCCCCCHHEECCCEEEECCCCHHHHHHHCCEEEECCEEEEEE
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGTIAL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCEEEE
IEDGDTIAIDIPNRSIQLQLSEAEIAARREAQEARGDKAWTPKNRQRQVSFALRAYASLA
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
TSADKGAVRDKSKLGG
CCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PKYRSATTTHGRNMAGARALWRATGMTDSDFGKPIITVVNSFTQFVPGHVHLRDLGKLV
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AEQIEASGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHCCCCCCCCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDDQ
CCCCCCHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SNQVERSACPTCGSCSGMFTANSMNCLIEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
CCCHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHH
ELTKRYYEQDDESALPRNIANKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD
HHHHHHHCCCCCHHCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEHHH
IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVLGILGELDRAGLLNRNVKNVLGLTLPQT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHHHHCCCCHHH
LEQYDITVTQDEAVKKMFRAGPAGIRTTQAFSQDCRWDSLDDDRAAGCIRSLEYAYSKDG
HHHHCCEEEHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
GLAVLYGNFAENGCIVKTAGVDDSILKFTGPVKVYESQDDAVEAILGGKVVEGDVVVIRY
CEEEEECCCCCCCCEEEECCCCCCHHEECCCEEEECCCCHHHHHHHCCEEEECCEEEEEE
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGTIAL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCEEEE
IEDGDTIAIDIPNRSIQLQLSEAEIAARREAQEARGDKAWTPKNRQRQVSFALRAYASLA
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
TSADKGAVRDKSKLGG
CCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA