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The map label for this gene is rfe

Identifier: 29143701

GI number: 29143701

Start: 3479111

End: 3480214

Strand: Reverse

Name: rfe

Synonym: t3379

Alternate gene names: 29143701

Gene position: 3480214-3479111 (Counterclockwise)

Preceding gene: 29143702

Following gene: 29143700

Centisome position: 72.63

GC content: 46.47

Gene sequence:

>1104_bases
GTGAAGTTACTCACCGCTTTATCTGAGCTAATCAGTATTTTTTTATTCACGACCATTTTTATTTTTCTTGCGCGAAAAGT
AGCCATAAAAATTGGTTTAGTGGATAAACCAAACTTTCGTAAACGACATCAGGGCGTTATCCCATTAGTCGGCGGGATTT
CTGTGTTTGCAGGAATCTGCTTTATGTTTGGCCTCTCGGATTACTACATACCGCACTTATCGCTATATCTTATTTGCGCT
GGTGTATTGGTTTTTGTTGGTGCGATGGATGACCGTTTTGATATCAGTGTAAAAATTCGTGCTGTGGTTCAGGCTGTTAT
TGCGGTTGTAATGATGGTTATCGCAAAACTTCACTTAGGTAGCCTTGGTTACATCTTTGGGCCTTGGGAGTTAGTGCTTG
GCCCCTTTGGCTATTTCCTTACTCTGTTTGCCGTATGGGCGGCGATCAACGCTTTCAATATGGTGGACGGTATTGACGGA
CTGCTGGGTGGGTTATCCAGCGTCTCATTTGCGGCGATGGGGCTGATTTTATGGTTTGATGGTCAGACTAGTCTGGCAAT
GTGGTGCTTTGCCATGATCGCCGCGATTCTGCCTTACATTATGCTTAATCTCGGTATTTTGGGGCGGCGTTATAAAGTCT
TTATGGGTGATGCCGGTAGTACACTGATTGGCTTTACCGTCATCTGGCTCTTACTGGAAACGACGCAGGGGAAAACTCAC
TCTATCAGCCCGGTTACCGCGCTATGGATTATCGCTATTCCATTGATGGATATGGTGGCGATTATGTATCGCCGTCTGCG
TAAAGGGATGAGCCCCTTTTCCCCTGATCGCCAACATATTCATCATTTGGTTATGCGAGCAGGGTTTACTTCCCGCCAGG
CATTTGTTCTTATTACGCTCGCAGCGGCGATTCTGGCGGGAGTAGGCGTAACGGCAGAATACTCTCATTTTGTTCCTGAA
TGGGTTATGTTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGCTACTGCATTAAGCGGGCCTGGAAGGTGGCGAGATT
TATTAAGCGTGTGAAGCGTCGCCTGCGCAGGCAACGTAAGAACCGGCCAAACTTAACCAAGTAA

Upstream 100 bases:

>100_bases
GTTTGCGGTAAAAAGTGTAATGTTCTAACAGGTCTCTTCTAGGAGCTGGCGTAACGCATTATACTTTAAGGAAATGATAT
ATGCTGAGAGCACATACACT

Downstream 100 bases:

>100_bases
ATGAGGATACGATGACACAACCATTACCGGGGGCACGCGCGGTGAGCGCTGAAAATGAACTGGATATTCGCGGGTTGTTT
CGTACTTTATGGGCTGGCAA

Product: undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-phosphate GlcNAc-1-phosphate transferase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKLLTALSELISIFLFTTIFIFLARKVAIKIGLVDKPNFRKRHQGVIPLVGGISVFAGICFMFGLSDYYIPHLSLYLICA
GVLVFVGAMDDRFDISVKIRAVVQAVIAVVMMVIAKLHLGSLGYIFGPWELVLGPFGYFLTLFAVWAAINAFNMVDGIDG
LLGGLSSVSFAAMGLILWFDGQTSLAMWCFAMIAAILPYIMLNLGILGRRYKVFMGDAGSTLIGFTVIWLLLETTQGKTH
SISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPDRQHIHHLVMRAGFTSRQAFVLITLAAAILAGVGVTAEYSHFVPE
WVMLVLFLLAFFLYGYCIKRAWKVARFIKRVKRRLRRQRKNRPNLTK

Sequences:

>Translated_367_residues
MKLLTALSELISIFLFTTIFIFLARKVAIKIGLVDKPNFRKRHQGVIPLVGGISVFAGICFMFGLSDYYIPHLSLYLICA
GVLVFVGAMDDRFDISVKIRAVVQAVIAVVMMVIAKLHLGSLGYIFGPWELVLGPFGYFLTLFAVWAAINAFNMVDGIDG
LLGGLSSVSFAAMGLILWFDGQTSLAMWCFAMIAAILPYIMLNLGILGRRYKVFMGDAGSTLIGFTVIWLLLETTQGKTH
SISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPDRQHIHHLVMRAGFTSRQAFVLITLAAAILAGVGVTAEYSHFVPE
WVMLVLFLLAFFLYGYCIKRAWKVARFIKRVKRRLRRQRKNRPNLTK
>Mature_367_residues
MKLLTALSELISIFLFTTIFIFLARKVAIKIGLVDKPNFRKRHQGVIPLVGGISVFAGICFMFGLSDYYIPHLSLYLICA
GVLVFVGAMDDRFDISVKIRAVVQAVIAVVMMVIAKLHLGSLGYIFGPWELVLGPFGYFLTLFAVWAAINAFNMVDGIDG
LLGGLSSVSFAAMGLILWFDGQTSLAMWCFAMIAAILPYIMLNLGILGRRYKVFMGDAGSTLIGFTVIWLLLETTQGKTH
SISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPDRQHIHHLVMRAGFTSRQAFVLITLAAAILAGVGVTAEYSHFVPE
WVMLVLFLLAFFLYGYCIKRAWKVARFIKRVKRRLRRQRKNRPNLTK

Specific function: Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP- C55). It is the first lipid-linked intermediate involved in enterobacter

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family. WecA subfamily

Homologues:

Organism=Escherichia coli, GI1790218, Length=367, Percent_Identity=87.4659400544959, Blast_Score=589, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECA_SALTI (Q8Z386)

Other databases:

- EMBL:   AL627279
- EMBL:   AE014613
- RefSeq:   NP_457829.1
- RefSeq:   NP_807043.1
- GeneID:   1071568
- GeneID:   1249892
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3379
- KEGG:   sty:STY3637
- HOGENOM:   HBG536396
- OMA:   IPLIDMI
- ProtClustDB:   PRK15119
- BioCyc:   SENT209261:T3379-MONOMER
- BioCyc:   SENT220341:STY3637-MONOMER
- InterPro:   IPR012750
- InterPro:   IPR000715
- PANTHER:   PTHR22926
- TIGRFAMs:   TIGR02380

Pfam domain/function: PF00953 Glycos_transf_4

EC number: 2.7.8.- [C]

Molecular weight: Translated: 41086; Mature: 41086

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1043dac4)-; HASH(0x10e08cf4)-; HASH(0x116288d8)-; HASH(0xe2fc6cc)-; HASH(0x11585e68)-; HASH(0x115b0a30)-; HASH(0x11c6275c)-; HASH(0x11ce1ae0)-; HASH(0x11d37484)-; HASH(0x11d668b8)-; HASH(0x110618d0)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCH
>Mature Secondary Structure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CCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504