Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 29143582

GI number: 29143582

Start: 3339008

End: 3340426

Strand: Direct

Name: gltD [H]

Synonym: t3248

Alternate gene names: 29143582

Gene position: 3339008-3340426 (Clockwise)

Preceding gene: 29143581

Following gene: 29143583

Centisome position: 69.68

GC content: 56.45

Gene sequence:

>1419_bases
ATGAGCCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCGAAGAAACCGCTGAAGCTCCGTAAGATCGA
GTTTGTTGAAATCTACGAACCGTTTTCTGAAGGTCAGGCGAAAGCCCAGGCGGATCGCTGTTTGTCCTGCGGGAACCCCT
ACTGCGAGTGGAAATGTCCGGTACATAACTACATCCCGAACTGGCTGAAACTGGCTAACGAAGGGCGTATTTTTGAGGCG
GCTGAGCTATCGCACCAGACCAACACCCTGCCGGAAGTGTGTGGCCGCGTCTGCCCGCAGGACCGCCTGTGCGAAGGCTC
CTGTACGCTGCACGATGAGTTCGGCGCAGTGACCATCGGTAACATTGAGCGCTATATCAACGATAAAGCGTTCGAAATGG
GCTGGCGCCCGGATATGACCGGCGTGCGCCAGACGGACAAACGGGTAGCGATTATCGGCGCTGGTCCGGCAGGGCTGGCG
TGCGCCGACGTGCTGACCCGCAACGGCGTGAAGGCGGTGGTCTTTGATCGCCATCCGGAAATCGGTGGTCTGCTGACCTT
CGGTATTCCAGCTTTCAAACTGGAAAAAGAGGTGATGACCCGTCGTCGCGAGATCTTCACCGGCATGGGTATTGAGTTCA
AACTTAATACCGAAGTGGGTCGTGATGTACAGCTTGAAGATCTGCTGAAAGATTACGATGCGGTATTCCTTGGCGTCGGC
ACCTATCAGTCGATGCGCGGCGGGCTGGAAAATGAAGATGCGGACGGGGTTTTCGACGCTCTACCGTTCCTGATCGCCAA
TACGAAGCAGATCATGGGGTTTGGCGAAACCAGCGACGAACCATATGTCAGCATGGAAGGCAAACGCGTGGTGGTGCTGG
GCGGCGGCGATACCGCAATGGACTGCGTACGCACTTCCATACGCCAGGGCGCAACGCACGTAACCTGTGCTTATCGTCGT
GATGAAGAGAACATGCCGGGTTCCAGACGCGAAGTGAAAAACGCGCGCGAGGAAGGTGTCGAGTTTCAGTTTAACGTTCA
ACCGCTGGGCATTGAAGTCAATGCCAACGGCAAAGTGAGCGGCGTAAAAATGGTGCGCACTGAAATGGGCGAACCGGATG
CCAAAGGCCGTCGGCGGGCGGAGATCGTCGCCGGTTCCGAGCATGTGGTTCCGGCTGATGCGGTGGTGATGGCGTTTGGT
TTTCGTCCACACAGCATGGAATGGCTGGCGAAACACAGCGTGGAACTGGACTCGCAGGGACGAATTATCGCGCCGGAACG
CAGCGATAATGCTTTCCAGACCAGCAACCCGAAAATCTTCGCCGGTGGCGACATCGTGCGCGGTTCCGATCTGGTGGTGA
CGGCGATTGCCGAAGGCCGTAAAGCGGCTGACGGGATTATGAACTACCTCGAAGTGTAA

Upstream 100 bases:

>100_bases
CGCGTTAGTTAAGCCCAAGTCCAGCGATGTCAAAGCCCTGTTGGGCCACCGTAGTCGTAGTGCGGCAGAATTGCGCGTGC
AGGCGCAGTAAGGAATTGAA

Downstream 100 bases:

>100_bases
CCCTTTCGGCTCCCCGGTTGATGCCTTGTGCTGATGGCTGGGGAGCCGATATTCCTCTCCTCAATATCTCTCCTGCCGAT
TCTGGAAACCCGCTCGTAAA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKLRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIGNIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLEDLLKDYDAVFLGVG
TYQSMRGGLENEDADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATHVTCAYRR
DEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFG
FRPHSMEWLAKHSVELDSQGRIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKLRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIGNIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLEDLLKDYDAVFLGVG
TYQSMRGGLENEDADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATHVTCAYRR
DEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFG
FRPHSMEWLAKHSVELDSQGRIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKLRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAA
ELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIGNIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLAC
ADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLEDLLKDYDAVFLGVGT
YQSMRGGLENEDADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATHVTCAYRRD
EENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFGF
RPHSMEWLAKHSVELDSQGRIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=480, Percent_Identity=27.9166666666667, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=95.3389830508475, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=66.3774403470716, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=58.8888888888889, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=31.447963800905, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=32.2352941176471, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=33.4008097165992, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71984108, Length=466, Percent_Identity=30.2575107296137, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=29.1925465838509, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=469, Percent_Identity=31.9829424307036, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=40, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=40, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=40, Blast_Score=298, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=40, Blast_Score=298, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24640763, Length=460, Percent_Identity=27.1739130434783, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18858217, Length=460, Percent_Identity=27.1739130434783, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52219; Mature: 52088

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKLRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCEEEEC
NIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLEDLLKDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCEEEEECC
TYQSMRGGLENEDADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHEECCCCCCCCEEEECCCEEEEECCCCHHH
DCVRTSIRQGATHVTCAYRRDEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCC
GVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
CEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKLRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCEEEEC
NIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLEDLLKDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCEEEEECC
TYQSMRGGLENEDADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHEECCCCCCCCEEEECCCEEEEECCCCHHH
DCVRTSIRQGATHVTCAYRRDEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCC
GVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
CEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]