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The map label for this gene is ispB [H]

Identifier: 29143556

GI number: 29143556

Start: 3313366

End: 3314337

Strand: Direct

Name: ispB [H]

Synonym: t3222

Alternate gene names: 29143556

Gene position: 3313366-3314337 (Clockwise)

Preceding gene: 29143551

Following gene: 29143557

Centisome position: 69.14

GC content: 53.4

Gene sequence:

>972_bases
ATGAATTTAGAAAAAATCAACGAGTTAACCGCGCAAGATATGGCGGGTGTCAATGCGACAATCCTTGAACAGCTTAATTC
CGACGTTCAACTGATTAATCAGCTAGGATATTACATTATTAGCGGCGGCGGAAAACGCATTCGTCCGATGATTGCCGTTC
TTGCCGCGCGTGCGGTGGGTTATCAGGGTAACGCGCACGTGACTATCGCCGCTCTGATCGAGTTTATCCACACGGCGACG
CTGCTACATGATGATGTGGTGGATGAATCTGATATGCGTCGCGGCAAAGCGACGGCAAATGCCGCTTTCGGTAATGCCGC
CAGCGTGTTGGTCGGCGACTTTATCTATACCCGCGCCTTCCAGATGATGACCAGCCTCGGCTCGTTAAAAGTGCTGGAAG
TGATGTCGGAAGCGGTCAATGTGATTGCAGAAGGCGAAGTGCTGCAATTGATGAACGTGAATGACCCGGACATTACCGAA
GAAAATTACATGCGGGTGATTTACAGTAAAACGGCCCGTCTGTTCGAAGCCGCCGCCCAGTGCTCCGGTATTCTTGCCGG
TTGTACGCCTGAGCAAGAGAAAGGGTTGCAGGACTATGGCCGCTACCTTGGTACGGCTTTCCAGCTCATTGACGATCTGC
TGGATTACAGTGCCGACGGCGAGCATCTCGGTAAAAATGTGGGTGATGACCTCAATGAGGGCAAACCTACCTTACCGTTG
CTTCACGCCATGCGCCACGGTACGCCAGAACAGTCAGCGATGATCCGTACCGCTATTGAACAAGGTAATGGCCGTCATCT
TCTGGAACCGGTTCTGGAAGCGATGACCACCTGCGGCTCGCTGGAATGGACGCGTCAGCGAGCGGAAGAAGAAGCCGACA
AAGCGATATCCGCGTTGCAGATATTGCCGGATACCCCATGGCGTGAGGCGCTAATCGGTCTCGCACACATCGCGGTGCAG
CGCGATCGTTGA

Upstream 100 bases:

>100_bases
CGGCGTTTTTTCAGTACAATCAGGGTATATTTCTAACCATAAACCCTACGTTACCTCATTCAGACATGGGATAATCGGAG
CGATAAGCCCGGCTTTTGCG

Downstream 100 bases:

>100_bases
TGCCATTCTCTTATCCCGCGCACTGCGCGGGATATCCCATTAAGTTCTAATAAACTCCAGATACCCTATTGCCTGTCTTT
TCGTATATTCATATACGGCT

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIISGGGKRIRPMIAVLAARAVGYQGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEQEKGLQDYGRYLGTAFQLIDDLLDYSADGEHLGKNVGDDLNEGKPTLPL
LHAMRHGTPEQSAMIRTAIEQGNGRHLLEPVLEAMTTCGSLEWTRQRAEEEADKAISALQILPDTPWREALIGLAHIAVQ
RDR

Sequences:

>Translated_323_residues
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIISGGGKRIRPMIAVLAARAVGYQGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEQEKGLQDYGRYLGTAFQLIDDLLDYSADGEHLGKNVGDDLNEGKPTLPL
LHAMRHGTPEQSAMIRTAIEQGNGRHLLEPVLEAMTTCGSLEWTRQRAEEEADKAISALQILPDTPWREALIGLAHIAVQ
RDR
>Mature_323_residues
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIISGGGKRIRPMIAVLAARAVGYQGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEQEKGLQDYGRYLGTAFQLIDDLLDYSADGEHLGKNVGDDLNEGKPTLPL
LHAMRHGTPEQSAMIRTAIEQGNGRHLLEPVLEAMTTCGSLEWTRQRAEEEADKAISALQILPDTPWREALIGLAHIAVQ
RDR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=324, Percent_Identity=31.7901234567901, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4758430, Length=265, Percent_Identity=22.6415094339623, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI83700220, Length=265, Percent_Identity=22.6415094339623, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=95.9752321981424, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1786623, Length=254, Percent_Identity=29.5275590551181, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=351, Percent_Identity=32.7635327635328, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=300, Percent_Identity=33.6666666666667, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=25.1101321585903, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24651612, Length=299, Percent_Identity=32.1070234113712, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI281365769, Length=285, Percent_Identity=24.2105263157895, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24660002, Length=285, Percent_Identity=24.2105263157895, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35267; Mature: 35267

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIISGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHC
YQGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEQEKGLQDYGRYLGTAFQLIDDLLDYSADGEHLGKNVGDDLNEGKPTLPL
HCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMRHGTPEQSAMIRTAIEQGNGRHLLEPVLEAMTTCGSLEWTRQRAEEEADKAISALQ
HHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ILPDTPWREALIGLAHIAVQRDR
HCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIISGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHC
YQGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEQEKGLQDYGRYLGTAFQLIDDLLDYSADGEHLGKNVGDDLNEGKPTLPL
HCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMRHGTPEQSAMIRTAIEQGNGRHLLEPVLEAMTTCGSLEWTRQRAEEEADKAISALQ
HHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ILPDTPWREALIGLAHIAVQRDR
HCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]