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The map label for this gene is garD [H]

Identifier: 29143507

GI number: 29143507

Start: 3261322

End: 3262893

Strand: Direct

Name: garD [H]

Synonym: t3170

Alternate gene names: 29143507

Gene position: 3261322-3262893 (Clockwise)

Preceding gene: 29143496

Following gene: 29143510

Centisome position: 68.06

GC content: 56.81

Gene sequence:

>1572_bases
ATGGCCAATATCGAAATTCGCCAGGAATCGCCATCGGCGTTTTATATAAAAGTACATGAAACCGATAATGTCGCAATTAT
TGTTAATGACCACGGCCTTAAAGCCGGAACACGTTTTCCGGATGGCCTGGAACTGACTGAACATATTCCGCAAGGCCATA
AAGTCGCTCTGACGGATATCCCCGCTCACGGCGAAATCATCCGTTATGGCGAAGTCATCGGTTATGCGGTACGCGACATC
CCGCGCGGAAGCTGGATCGACGAGTCGCTGGTTGAACTGCCAAAAGCGCCGCCGCTGAATACGCTACCGCTGGCGACCAA
AGTACCGGAACCGCTGCCGCCGCTGGAAGGCTACACGTTTGAGGGCTATCGTAACGCCGATGGCAGCGTCGGCACCAAAA
ATCTGCTTGGCATTACCACCAGCGTGCACTGTGTGGCGGGCGTGGTCGATTATGTGGTGAAAGTGATTGAGCGCGATCTC
CTGCCCAAATACCCGAATGTCGATGGCGTTGTAGGGCTAAACCATCTGTACGGCTGCGGCGTGGCAATCAACGCGCCGGC
GGCGGTCGTGCCGATTCGCACAATTCATAATATCGCGCTCAACCCCAATTTTGGCGGCGAAGTGATGGTGATTGGCCTCG
GTTGCGAAAAACTGCAGCCGGAACGACTGCTGGAAGGCACCGAGGATGTCCCCGCCATCGCCGTTGAAAACGCCAGTATT
GTGCGTTTACAGGACGAGCAGCATGTTGGCTTTAAATCAATGGTCGACGATATTTTGCGGGTCGCCGAACGCCATCTGAC
GAAACTGAACCAGCGCCAGCGCGAAACCTGCCCGGCCTCCGAGCTGGTCGTCGGGATGCAATGCGGCGGCAGCGATGCGT
TTTCTGGCGTCACGGCAAACCCGGCGGTGGGTTATGCTTCCGATCTGCTGGTACGCTGCGGCGCGACCGTCATGTTCTCG
GAGGTCACCGAAGTGCGCGATGCCATTCATCTTCTGACGCCGCGCGCCATCAACGAAGCCGTGGGTAAACGCCTACTGGA
TGAAATGGCCTGGTACGATAACTACCTTGATATGGGGAAAACCGATCGCAGCGCTAACCCGTCCCCCGGCAACAAAAAAG
GCGGCCTGGCGAACGTCGTGGAAAAAGCGCTGGGCTCTATCGCTAAATCGGGTAAGAGCGCCATTGTCGAAGTATTGTCG
CCGGGCCAGCGCCCCACCAAACGCGGCCTGATTTACGCCGCGACGCCCGCCAGCGATTTCGTCTGCGGCACGCAGCAGGT
CGCTTCCGGCATTACCGTACAGGTCTTTACCACCGGACGCGGCACGCCGTACGGGCTGATGGCGGTGCCGGTCATTAAAA
TGGCGACGCGTACCGAGCTGGCGAACCGCTGGTATGACTTAATGGATATCAATGCAGGCACCATCGCCACGGGTGAAGAG
ACAATCGAAGATGTGGGCTGGAAGCTATTTCACTTTATTCTCGATGTCGCCAGCGGCCGGAAAAAGACTTTCTCAGATCA
ATGGGGATTGCATAACCAGCTGGCAGTGTTTAACCCCGCACCTGTGACCTGA

Upstream 100 bases:

>100_bases
TTATGCATGAACTTTATTTCTTATATTGAGATTTAACGATTTAACCATAACCAAAATAGATAATCCTATTTCTATCATCC
AGGCTGGAGAATGTTGAACA

Downstream 100 bases:

>100_bases
TCTATTCTGGAAAGCGCCTCGCAAAACATGGCTTAAGGGCCATGTTTTCATATGTTTGATAAACTGCGCCATGTTCATAC
CGGATATGGCAAAAACACCT

Product: D-galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MANIEIRQESPSAFYIKVHETDNVAIIVNDHGLKAGTRFPDGLELTEHIPQGHKVALTDIPAHGEIIRYGEVIGYAVRDI
PRGSWIDESLVELPKAPPLNTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKVIERDL
LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNIALNPNFGGEVMVIGLGCEKLQPERLLEGTEDVPAIAVENASI
VRLQDEQHVGFKSMVDDILRVAERHLTKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFS
EVTEVRDAIHLLTPRAINEAVGKRLLDEMAWYDNYLDMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLS
PGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWYDLMDINAGTIATGEE
TIEDVGWKLFHFILDVASGRKKTFSDQWGLHNQLAVFNPAPVT

Sequences:

>Translated_523_residues
MANIEIRQESPSAFYIKVHETDNVAIIVNDHGLKAGTRFPDGLELTEHIPQGHKVALTDIPAHGEIIRYGEVIGYAVRDI
PRGSWIDESLVELPKAPPLNTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKVIERDL
LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNIALNPNFGGEVMVIGLGCEKLQPERLLEGTEDVPAIAVENASI
VRLQDEQHVGFKSMVDDILRVAERHLTKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFS
EVTEVRDAIHLLTPRAINEAVGKRLLDEMAWYDNYLDMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLS
PGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWYDLMDINAGTIATGEE
TIEDVGWKLFHFILDVASGRKKTFSDQWGLHNQLAVFNPAPVT
>Mature_522_residues
ANIEIRQESPSAFYIKVHETDNVAIIVNDHGLKAGTRFPDGLELTEHIPQGHKVALTDIPAHGEIIRYGEVIGYAVRDIP
RGSWIDESLVELPKAPPLNTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKVIERDLL
PKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNIALNPNFGGEVMVIGLGCEKLQPERLLEGTEDVPAIAVENASIV
RLQDEQHVGFKSMVDDILRVAERHLTKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSE
VTEVRDAIHLLTPRAINEAVGKRLLDEMAWYDNYLDMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSP
GQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWYDLMDINAGTIATGEET
IEDVGWKLFHFILDVASGRKKTFSDQWGLHNQLAVFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=523, Percent_Identity=93.4990439770555, Blast_Score=1014, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=516, Percent_Identity=32.3643410852713, Blast_Score=222, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 56381; Mature: 56250

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIEIRQESPSAFYIKVHETDNVAIIVNDHGLKAGTRFPDGLELTEHIPQGHKVALTDI
CCCEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEEEEC
PAHGEIIRYGEVIGYAVRDIPRGSWIDESLVELPKAPPLNTLPLATKVPEPLPPLEGYTF
CCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
EGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKVIERDLLPKYPNVDGVVGLNHLYGCG
CCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHCCC
VAINAPAAVVPIRTIHNIALNPNFGGEVMVIGLGCEKLQPERLLEGTEDVPAIAVENASI
EEECCCCEEEEHHHHHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCCCCEEEECCCEE
VRLQDEQHVGFKSMVDDILRVAERHLTKLNQRQRETCPASELVVGMQCGGSDAFSGVTAN
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCC
PAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAINEAVGKRLLDEMAWYDNYLDMGK
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCC
VCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWYDLMDINAGTIATGEE
CCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCHH
TIEDVGWKLFHFILDVASGRKKTFSDQWGLHNQLAVFNPAPVT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
ANIEIRQESPSAFYIKVHETDNVAIIVNDHGLKAGTRFPDGLELTEHIPQGHKVALTDI
CCEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEEEEC
PAHGEIIRYGEVIGYAVRDIPRGSWIDESLVELPKAPPLNTLPLATKVPEPLPPLEGYTF
CCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
EGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKVIERDLLPKYPNVDGVVGLNHLYGCG
CCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHCCC
VAINAPAAVVPIRTIHNIALNPNFGGEVMVIGLGCEKLQPERLLEGTEDVPAIAVENASI
EEECCCCEEEEHHHHHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCCCCEEEECCCEE
VRLQDEQHVGFKSMVDDILRVAERHLTKLNQRQRETCPASELVVGMQCGGSDAFSGVTAN
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCC
PAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAINEAVGKRLLDEMAWYDNYLDMGK
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCC
VCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWYDLMDINAGTIATGEE
CCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCHH
TIEDVGWKLFHFILDVASGRKKTFSDQWGLHNQLAVFNPAPVT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]