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The map label for this gene is tdcE [H]

Identifier: 29143499

GI number: 29143499

Start: 3249431

End: 3251725

Strand: Reverse

Name: tdcE [H]

Synonym: t3161

Alternate gene names: 29143499

Gene position: 3251725-3249431 (Counterclockwise)

Preceding gene: 29143500

Following gene: 29143498

Centisome position: 67.86

GC content: 54.86

Gene sequence:

>2295_bases
ATGAAGGTAAATATCGATACCAGCGATATGCTGTATGCCGAAGCATGGCGTGATTTTAAAGGTACGGACTGGAAAGAAGA
AATTAATGTCTGTGATTTTATTCAGCATAACTATACGCCCTATGAGGGGGATGAATCTTTTCTCGCCGACGCGACGCCAG
CGACCACAGCGTTGTGGGAAAAGGTGATGGCGGGAATACGTATCGAGAACGCGACCCATGCGCCGGTGGATTTCGATACC
AACATCGCGACGACGATTACCGCGCACGATGCGGGGTATATTGAAAAAGAGCTGGAAAAAATCGTCGGTTTACAAACGGA
TAAGCCGCTTAAACGCGCTTTGCATCCGTTTGGCGGCGTTAACATGATCAAAAGTTCATTCCACGCTTATGGCCGGGAAA
TGGACGCCGATTTTGAATATACCTTTACCGACCTGCGGAAAACCCATAATCAGGGCGTATTCGATGTTTACTCGCCGGAT
ATGCTGCGCTGCCGTAAATCCAGCGTATTAACCGGTTTGCCGGATGGTTATGGTCGCGGGCGCATTATTGGCGATTATCG
CCGCGTCGCGCTGTATGGCATCCGTTATCTGGTGCGCGAACGCGAGCTGCAATTCGCCGACCTGCAGTCGAATCTGGAGC
GGGGACAGAATCTTGAGGCGACGATTCGCCTGCGTGAGGAGTTGGCGGAGCACCGTCGCGCGCTGCTGCAAATGCAGGAG
ATGGCGGCGAAATACGGCTATGATATCTCCCGCCCGGCGCGGAACGCGCAGGAAGCGGTACAGTGGCTCTACTTCGCTTA
TCTGGCGGCGGTAAAATCGCAAAATGGCGGTGCAATGTCGCTGGGAAGAACCGCCTCGTTCCTTGATATCTACATTGAGC
GCGATTTCAACGCGGGTCTTCTGACAGAGCAGCAGGCGCAGGAGCTGATCGACCATTTCATCATGAAGATCCGCATGGTG
CGCTTCCTGCGCACGCCGGAATTTGATTCGCTCTTTTCCGGCGATCCGATCTGGGCCACCGAAGTTATTGGCGGGATGGG
GCTTGATGGCCGCACGCTGGTGACGAAGAACTCTTTCCGTTATCTGCATACCCTGCACACGATGGGGCCAGCGCCGGAGC
CGAACCTGACCATACTGTGGTCAGAAGCGTTGCCGGTGGCATTCAAGAAATATGCTGCGCAGGTCTCCATCGTGACCTCA
TCTTTGCAGTATGAAAACGACGATTTAATGCGTACCGACTTTAACAGCGACGATTACGCCATCGCTTGTTGCGTCAGCCC
GATGGTCATCGGTAAGCAGATGCAGTTCTTCGGCGCGCGCGCCAACCTTGCCAAAACGTTGCTGTACGCTATCAATGGCG
GCGTTGACGAGAAACTGAAAATCCAGGTTGGGCCGAAAACCGCGCCGTTAACGGATGAGGTGCTGGATTATGACGCAGTA
ATGGAGAGCCTCGACCATTTTATGGACTGGCTGGCGGTGCAGTACATTAGCGCGCTGAACATCATTCACTATATGCACGA
TAAGTACAGCTATGAAGCTTCGCTGATGGCGCTGCACGACCGTGATGTGTATCGGACGATGGCGTGCGGCATCGCCGGAC
TGTCGGTGGCGGCGGATTCCCTATCCGCCATCAGGTACGCTCAGGTGAAGCCGATTCGCGACGAAAATGGCCTTGCCATC
GACTTTGCTATTGAAGGCGAGTATCCGCAGTACGGCAACAACGATGAGCGCGTGGACAGCATCGCCTGCGACCTGGTGAA
ACGCTTTATGCAAAAAATTAGCGTGTTGCCGACGTATCGCAATGCGGTGCCGACGCAGTCCATTTTGACCATCACCTCGA
ACGTCGTGTACGGGCAGAAGACCGGCAATACGCCGGATGGACGCCGCGCCGGAACGCCGTTCGCGCCGGGGGCGAACCCG
ATGCACGGTCGTGACCGCAAAGGGGCGGTGGCCTCGCTGACGTCGGTGGCGAAACTGCCGTTTACCTACGCCAAAGACGG
TATTTCGTACACCTTCTCGATTGTGCCTGCGGCGCTCGGGAAAGAAGATGCGGTGCGGAAAACCAATCTTGTCGGCCTGC
TGGACGGCTATTTCCATCATGAAGCACAGGTAGAAGGCGGGCAGCATCTGAACGTCAATGTCATGAACCGTGAAATGTTG
CTGGATGCGATTGAACATCCGGAAAACTACCCGAACCTGACGATCCGCGTTTCCGGCTATGCCGTGCGCTTCAATGCGCT
GACTCGTGAGCAGCAACAGGACGTTATTTCGCGCACCTTTACTCAGGCGATGTAA

Upstream 100 bases:

>100_bases
AGAGAAAATGATTGCCCTTGATGCCATTCATTTAGGCAACGTGAAAGCACCAGTGGAATTTGCATAACTTAACGGCTGTT
CTGTAGAGAGATTATTTTTC

Downstream 100 bases:

>100_bases
CGTGAACGGGGCGATCGTGTGGATCGCCCCGCGACGGATCTTCACTGAACGGTAATCTTAGGGGGAGATATGATTAGCGC
ATTTGATATTTTTAAGATTG

Product: formate acetyltransferase

Products: CoA; pyruvate

Alternate protein names: Keto-acid formate-lyase [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVCDFIQHNYTPYEGDESFLADATPATTALWEKVMAGIRIENATHAPVDFDT
NIATTITAHDAGYIEKELEKIVGLQTDKPLKRALHPFGGVNMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPD
MLRCRKSSVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSNLERGQNLEATIRLREELAEHRRALLQMQE
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGLLTEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIRYAQVKPIRDENGLAI
DFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM

Sequences:

>Translated_764_residues
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVCDFIQHNYTPYEGDESFLADATPATTALWEKVMAGIRIENATHAPVDFDT
NIATTITAHDAGYIEKELEKIVGLQTDKPLKRALHPFGGVNMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPD
MLRCRKSSVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSNLERGQNLEATIRLREELAEHRRALLQMQE
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGLLTEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIRYAQVKPIRDENGLAI
DFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM
>Mature_764_residues
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVCDFIQHNYTPYEGDESFLADATPATTALWEKVMAGIRIENATHAPVDFDT
NIATTITAHDAGYIEKELEKIVGLQTDKPLKRALHPFGGVNMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPD
MLRCRKSSVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSNLERGQNLEATIRLREELAEHRRALLQMQE
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGLLTEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIRYAQVKPIRDENGLAI
DFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI48994926, Length=764, Percent_Identity=93.3246073298429, Blast_Score=1499, Evalue=0.0,
Organism=Escherichia coli, GI1787131, Length=752, Percent_Identity=78.9893617021277, Blast_Score=1281, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=627, Percent_Identity=27.2727272727273, Blast_Score=176, Evalue=6e-45,
Organism=Escherichia coli, GI1790388, Length=641, Percent_Identity=25.7410296411856, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=73.4375, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: 2.3.1.54

Molecular weight: Translated: 85870; Mature: 85870

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVCDFIQHNYTPYEGDESFLADATPATTALWE
CEEECCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
KVMAGIRIENATHAPVDFDTNIATTITAHDAGYIEKELEKIVGLQTDKPLKRALHPFGGV
HHHHCEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCH
NMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPDMLRCRKSSVLTGLPDGYGRG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCCCC
RIIGDYRRVALYGIRYLVRERELQFADLQSNLERGQNLEATIRLREELAEHRRALLQMQE
CEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LTEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEEECCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIRYAQVKPIRDENGLAIDFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYR
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEEECHHHHH
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM
HHHHHCCCCCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVCDFIQHNYTPYEGDESFLADATPATTALWE
CEEECCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
KVMAGIRIENATHAPVDFDTNIATTITAHDAGYIEKELEKIVGLQTDKPLKRALHPFGGV
HHHHCEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCH
NMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPDMLRCRKSSVLTGLPDGYGRG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCCCC
RIIGDYRRVALYGIRYLVRERELQFADLQSNLERGQNLEATIRLREELAEHRRALLQMQE
CEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LTEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEEECCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIRYAQVKPIRDENGLAIDFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYR
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEEECHHHHH
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM
HHHHHCCCCCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9484901 [H]