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The map label for this gene is cca [H]

Identifier: 29143465

GI number: 29143465

Start: 3212217

End: 3213458

Strand: Direct

Name: cca [H]

Synonym: t3124

Alternate gene names: 29143465

Gene position: 3212217-3213458 (Clockwise)

Preceding gene: 29143464

Following gene: 29143468

Centisome position: 67.03

GC content: 58.94

Gene sequence:

>1242_bases
GTGAAGATTTATCTGGTCGGTGGTGCGGTTCGGGATGCGTTGTTAGGGCTACCGGTTAAAGATAAAGATTGGGTCGTTGT
CGGCGCCACGCCGCAGGAGATGCTTGATGCGGGCTACCAGCAGGTAGGCCGTGATTTTCCTGTGTTTCTCCACCCGCAAA
CTCACGAAGAATACGCGCTGGCGCGTACCGAGCGTAAATCCGGTTCCGGCTACACGGGTTTCACCTGCTATGCCGCGCCG
GACGTTACCCTGGAAGCCGACCTCCAGCGTCGCGATCTGACCATTAATGCTCTGGCGCGCGACGATGACGGACAGATTAT
CGACCCTTACCACGGGCGCCGGGATCTGGAGGCGCGTCTACTGCGCCATGTCTCTCCGGCGTTTGGCGAAGATCCCTTGC
GCGTATTGCGCGTGGCGCGTTTTGCCGCCCGTTACGCTCATCTTAGTTTCCGTATCGCCGATGAAACACTGACGCTAATG
CGCGAAATGACCGCGGCAGGCGAGCTGGAACATCTGACGCCGGAACGGGTATGGAAAGAGACGGAAAATGCGCTTACCAC
CCGCAATCCGCAGGTTTACTTCCAGGTATTGCGCGACTGCGGCGCCCTGCGCGTCCTGTTTCCGGAAATCGACGCCCTGT
TTGGCGTACCCGCGCCGGCAAAGTGGCATCCGGAAATTGATACCGGAGTTCATACGTTGATGACGCTGTCGATGGCCGCC
ATGCTCAGTCCGCAGCTTGATGTCCGTTTCGCCACGCTTTGTCATGATGTGGGCAAAGGCTTAACGCCGAAAAACCTCTG
GCCGCGCCATCATGGCCACGGCCCGGTGGGCGTGAAACTGGTCGAACAGTTATGCCAGCGTTTGCGCGTCCCCAATGACC
TGCGTGATTTAGCTAAACTGGTCGCCGCGTACCACGATCTGATTCACACCTTTCCGATACTCCAGCCGAAAACGATCGTT
AAGCTGTTTGACGCCATTGATGCATGGCGTAAACCGCAGCGCGTCGAGCAGATTGCGCTGACCAGCGAGGCCGATGTCCG
TGGACGTACCGGCTTTGAAGCGTCGGATTACCCGCAGGGGCGCTGGCTGCGCGAAGCCTGGCAGGTGGCCCAAGCGGTAC
CAACGAAAGAGGTGGTGGAGGCCGGATTTAAGGGCATTGAGATTCGCGAGGAGCTGACGAAGCGACGAATTGCAGCGGTC
GCCAACTGGAAAGAGAAGCGCTGCCCGAACCCCGCCAGCTAA

Upstream 100 bases:

>100_bases
TCCCAAGCCGTAAACGCAAAGACCGCTGGATGAACTAAATCGCCTTCTCTGCGGCACTGTCTTATCATTAGAGGATGATC
GTTTTTAAAAGGAAAGTGGC

Downstream 100 bases:

>100_bases
ACGCCATCCGGCACGATAAATTGCCTGATGGCGCTGCGCTTATCAGACCGACAAAACTGTTAGGCCGGACAAGGCGGTAC
GCCGCCATCCGGCACGGTCA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKIYLVGGAVRDALLGLPVKDKDWVVVGATPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEADLQRRDLTINALARDDDGQIIDPYHGRRDLEARLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLTLM
REMTAAGELEHLTPERVWKETENALTTRNPQVYFQVLRDCGALRVLFPEIDALFGVPAPAKWHPEIDTGVHTLMTLSMAA
MLSPQLDVRFATLCHDVGKGLTPKNLWPRHHGHGPVGVKLVEQLCQRLRVPNDLRDLAKLVAAYHDLIHTFPILQPKTIV
KLFDAIDAWRKPQRVEQIALTSEADVRGRTGFEASDYPQGRWLREAWQVAQAVPTKEVVEAGFKGIEIREELTKRRIAAV
ANWKEKRCPNPAS

Sequences:

>Translated_413_residues
MKIYLVGGAVRDALLGLPVKDKDWVVVGATPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEADLQRRDLTINALARDDDGQIIDPYHGRRDLEARLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLTLM
REMTAAGELEHLTPERVWKETENALTTRNPQVYFQVLRDCGALRVLFPEIDALFGVPAPAKWHPEIDTGVHTLMTLSMAA
MLSPQLDVRFATLCHDVGKGLTPKNLWPRHHGHGPVGVKLVEQLCQRLRVPNDLRDLAKLVAAYHDLIHTFPILQPKTIV
KLFDAIDAWRKPQRVEQIALTSEADVRGRTGFEASDYPQGRWLREAWQVAQAVPTKEVVEAGFKGIEIREELTKRRIAAV
ANWKEKRCPNPAS
>Mature_413_residues
MKIYLVGGAVRDALLGLPVKDKDWVVVGATPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEADLQRRDLTINALARDDDGQIIDPYHGRRDLEARLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLTLM
REMTAAGELEHLTPERVWKETENALTTRNPQVYFQVLRDCGALRVLFPEIDALFGVPAPAKWHPEIDTGVHTLMTLSMAA
MLSPQLDVRFATLCHDVGKGLTPKNLWPRHHGHGPVGVKLVEQLCQRLRVPNDLRDLAKLVAAYHDLIHTFPILQPKTIV
KLFDAIDAWRKPQRVEQIALTSEADVRGRTGFEASDYPQGRWLREAWQVAQAVPTKEVVEAGFKGIEIREELTKRRIAAV
ANWKEKRCPNPAS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=411, Percent_Identity=89.7810218978102, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI87081691, Length=323, Percent_Identity=27.8637770897833, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281360112, Length=216, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357337, Length=216, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46563; Mature: 46563

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDALLGLPVKDKDWVVVGATPQEMLDAGYQQVGRDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGSGYTGFTCYAAPDVTLEADLQRRDLTINALARDDDGQIIDPYHGRRDLEARL
HHHHCCCCCCCCCEEEEECCCCEEECCCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHH
LRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLTLMREMTAAGELEHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCHHCCCHHHHHHH
TENALTTRNPQVYFQVLRDCGALRVLFPEIDALFGVPAPAKWHPEIDTGVHTLMTLSMAA
HHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPQLDVRFATLCHDVGKGLTPKNLWPRHHGHGPVGVKLVEQLCQRLRVPNDLRDLAKL
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
VAAYHDLIHTFPILQPKTIVKLFDAIDAWRKPQRVEQIALTSEADVRGRTGFEASDYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RWLREAWQVAQAVPTKEVVEAGFKGIEIREELTKRRIAAVANWKEKRCPNPAS
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDALLGLPVKDKDWVVVGATPQEMLDAGYQQVGRDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGSGYTGFTCYAAPDVTLEADLQRRDLTINALARDDDGQIIDPYHGRRDLEARL
HHHHCCCCCCCCCEEEEECCCCEEECCCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHH
LRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLTLMREMTAAGELEHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCHHCCCHHHHHHH
TENALTTRNPQVYFQVLRDCGALRVLFPEIDALFGVPAPAKWHPEIDTGVHTLMTLSMAA
HHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPQLDVRFATLCHDVGKGLTPKNLWPRHHGHGPVGVKLVEQLCQRLRVPNDLRDLAKL
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
VAAYHDLIHTFPILQPKTIVKLFDAIDAWRKPQRVEQIALTSEADVRGRTGFEASDYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RWLREAWQVAQAVPTKEVVEAGFKGIEIREELTKRRIAAVANWKEKRCPNPAS
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA