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The map label for this gene is imm [H]

Identifier: 29143382

GI number: 29143382

Start: 3121018

End: 3121296

Strand: Direct

Name: imm [H]

Synonym: t3038

Alternate gene names: 29143382

Gene position: 3121018-3121296 (Clockwise)

Preceding gene: 29143381

Following gene: 29143383

Centisome position: 65.13

GC content: 35.48

Gene sequence:

>279_bases
ATGGAACTAAAAAATAACCTTGAAGATTATACAGAAGATGAGTTTATCGAGTTTTTAAATAATTTTTTTGAGCCTCCTGA
AGAGTTGACTGGAGATGAATTATCGAAGTTTATTGATAATCTTCTACGCCATTTTAATAAAATTACTCAACACCCTGATG
GCGGGGATTTGATATTTTATCCATCAGAGGAACGAGAAGATAGTCCTGAAGGAGTTATTGAAGAGCTAAAACGCTGGCGG
AAGTCTCAGAGACTCCCCTGTTTTAAGGAAAATAAGTAG

Upstream 100 bases:

>100_bases
GAAAGATCCAGTATGGAGGAATGGTGTTTGATGTTGATAACCTTATGATAAACACTCCTAAAAATCATATACGATTGCAT
AAGTAAGGTTTGAGGTGAAA

Downstream 100 bases:

>100_bases
CGATCAGTGGTGAGGAGTTTGTCAACCGGAACCGGGATGATAATGCAGTGACCGTTAAAAGGCAAAAATATGTCAGTTAA
GGGAGTAACTATCAAAGGCA

Product: bacteriocin immunity protein

Products: NA

Alternate protein names: ImmE7; Microcin-E7 immunity protein [H]

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MELKNNLEDYTEDEFIEFLNNFFEPPEELTGDELSKFIDNLLRHFNKITQHPDGGDLIFYPSEEREDSPEGVIEELKRWR
KSQRLPCFKENK

Sequences:

>Translated_92_residues
MELKNNLEDYTEDEFIEFLNNFFEPPEELTGDELSKFIDNLLRHFNKITQHPDGGDLIFYPSEEREDSPEGVIEELKRWR
KSQRLPCFKENK
>Mature_92_residues
MELKNNLEDYTEDEFIEFLNNFFEPPEELTGDELSKFIDNLLRHFNKITQHPDGGDLIFYPSEEREDSPEGVIEELKRWR
KSQRLPCFKENK

Specific function: This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleave

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000290
- ProDom:   PD007225 [H]

Pfam domain/function: PF01320 Colicin_Pyocin [H]

EC number: NA

Molecular weight: Translated: 11080; Mature: 11080

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKNNLEDYTEDEFIEFLNNFFEPPEELTGDELSKFIDNLLRHFNKITQHPDGGDLIFY
CCCCCHHHHCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PSEEREDSPEGVIEELKRWRKSQRLPCFKENK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MELKNNLEDYTEDEFIEFLNNFFEPPEELTGDELSKFIDNLLRHFNKITQHPDGGDLIFY
CCCCCHHHHCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PSEEREDSPEGVIEELKRWRKSQRLPCFKENK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2045785; 8692833; 9135159; 10368275 [H]