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The map label for this gene is pilT [H]

Identifier: 29143361

GI number: 29143361

Start: 3101125

End: 3102105

Strand: Reverse

Name: pilT [H]

Synonym: t3011

Alternate gene names: 29143361

Gene position: 3102105-3101125 (Counterclockwise)

Preceding gene: 29143367

Following gene: 29143360

Centisome position: 64.74

GC content: 57.49

Gene sequence:

>981_bases
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGATACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Upstream 100 bases:

>100_bases
TCTTCTGAAGAGCGGCCACAACGCGTCGCCGCGGCGGAGATTTTGTCCCGGATGTGTGCCAGGTTATGCGCGATATCGTT
CATTTTCCGAGGATGTTTAT

Downstream 100 bases:

>100_bases
CGAAGATGGCGGCGTAGGGAAGGGGAAGTGTTGACTGGCGCGGTGGATTGAACGGCATACAGGCTTGTATCCTGGCGAAC
CGGCGTTAAAATCGCAGCTT

Product: Type II secretion, ATP-binding, protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Sequences:

>Translated_326_residues
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL
>Mature_326_residues
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Specific function: Unknown

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=326, Percent_Identity=78.8343558282209, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1789723, Length=185, Percent_Identity=43.2432432432432, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786296, Length=273, Percent_Identity=33.6996336996337, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 36113; Mature: 36113

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQW
CCHHHEEEEEEECCCCCEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RTHGQLDFAVSLSGTRRLRASAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASEN
HCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHCCCHHHCCCCCCCHHHHCCCC
GLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIEYRYTSKRCLIQQREIGQHCA
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHH
TFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
HHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCCCCCCCHHCCCHHHHHHH
VIQTGQQQGMMTFAQSAQWRQAQGRL
HHHHCHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQW
CCHHHEEEEEEECCCCCEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RTHGQLDFAVSLSGTRRLRASAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASEN
HCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHCCCHHHCCCCCCCHHHHCCCC
GLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIEYRYTSKRCLIQQREIGQHCA
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHH
TFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
HHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCCCCCCCHHCCCHHHHHHH
VIQTGQQQGMMTFAQSAQWRQAQGRL
HHHHCHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]