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The map label for this gene is visB [H]

Identifier: 29143325

GI number: 29143325

Start: 3066485

End: 3067663

Strand: Reverse

Name: visB [H]

Synonym: t2975

Alternate gene names: 29143325

Gene position: 3067663-3066485 (Counterclockwise)

Preceding gene: 29143326

Following gene: 29143324

Centisome position: 64.02

GC content: 60.64

Gene sequence:

>1179_bases
ATGAGCGTCATTATCGTTGGCGGCGGCATGGCAGGCGCAACGCTGGCGCTGGCCATCTCTCAGTTCAGTCATGGGACGTT
GCCGGTACACCTGATTGAAGCGAAAGCGCCTGAAGCCGACGGTCATCCCGGCTTTGACGCCAGAGCGATTGCGCTGGCGG
CGGGCACTTGCCAGCAACTGGCGCGAATTGGCGTCTGGCAGGCGATTTCCGATTGCGCAACGGCAATCAACACCGTACAC
GTCAGCGATCGCGGTCATGCCGGATTTGTGACTCTAGACGCGCAGGATTACAGCCTGGCAGCGTTGGGTCACGTTGCGGA
GCTGCATGATATCGGGCTGCGTCTGTTTGCGCTCTTGCGTAAAGCGCCAGGCGTTACGCTGCACTGCCCGGAGCGCGTAG
CCAGCGTGTCGCGTACCCAACAGCAGGTTAACGTCACTCTGGAAAACGGCAATGTGATAGCTGGCAGCGTGCTGGTGGCG
GCTAACGGTACCCACTCCGCGCTGGCATCAGCCTGCGGCGTTGACTGGCATCAGGAGCCTTACGAACAGTTAGCGGTGAT
AGCGAATGTCGCGACCGCTATCCCGCACCAGGGACGCGCCTTTGAACGTTTTACCCCCAATGGCCCGCTGGCGATGTTGC
CGATGTCGCATGGCCGATGTTCGCTGGTTTGGTGTCACCCGCTCGACCAGCGCGATGAGGTGCTGAGCTGGTCCGACGCG
CAGTTCTGTCATGCGTTGCAGGCCGCGTTCGGCTGGCGGCTGGGGCGAATTACTCAGGCGGGTAAGCGCAGCGCGTATCC
GCTTGCCCTGACTACGGCGGCGAAAGTGTTTACTCACCGGACTGTCGTGGTCGGCAATGCGGCGCAGACGTTGCATCCTA
TCGCCGGACAAGGGTTTAATCTCGGTCTGCGCGATGTCATGAGCCTGGCGGAAACGCTGGTCGCGGCACAGGACGCGGGC
GAGGATATTGGCGCCTGGCACGTGTTATCGCGCTATCAGCAGCGTCGTCAGGTTGACCGGGATACGACGATTGGCGTAAC
GAACGGTCTGGTCCATCTGTTTGCAAATCGTTGGGCGCCGCTGGTTATTGGGCGCAATATCGGTTTAATGGCGATGGAAT
TATTACCACCTGCGCGCGATGCGCTGGCACAGCGAACCTTAGGTTGGGTTGCGCGTTAA

Upstream 100 bases:

>100_bases
ATGACATTGTCATTACCGAAACCGGTAATGAAAACCTGACCGCTGGCGTTGTGAAGAAGGCGGATGACATTGAGGCATTA
ATGGCGGCGGCGCGGCAGCA

Downstream 100 bases:

>100_bases
GCAGATCTGTAGGCCGGATAAGGCGTTTACGCCGCCATCCGGCATCGTGTTCGTAAAAATGCCGGATGGCGGCGTAAACG
CCTTATCCGGCCTACGGTTT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVIIVGGGMAGATLALAISQFSHGTLPVHLIEAKAPEADGHPGFDARAIALAAGTCQQLARIGVWQAISDCATAINTVH
VSDRGHAGFVTLDAQDYSLAALGHVAELHDIGLRLFALLRKAPGVTLHCPERVASVSRTQQQVNVTLENGNVIAGSVLVA
ANGTHSALASACGVDWHQEPYEQLAVIANVATAIPHQGRAFERFTPNGPLAMLPMSHGRCSLVWCHPLDQRDEVLSWSDA
QFCHALQAAFGWRLGRITQAGKRSAYPLALTTAAKVFTHRTVVVGNAAQTLHPIAGQGFNLGLRDVMSLAETLVAAQDAG
EDIGAWHVLSRYQQRRQVDRDTTIGVTNGLVHLFANRWAPLVIGRNIGLMAMELLPPARDALAQRTLGWVAR

Sequences:

>Translated_392_residues
MSVIIVGGGMAGATLALAISQFSHGTLPVHLIEAKAPEADGHPGFDARAIALAAGTCQQLARIGVWQAISDCATAINTVH
VSDRGHAGFVTLDAQDYSLAALGHVAELHDIGLRLFALLRKAPGVTLHCPERVASVSRTQQQVNVTLENGNVIAGSVLVA
ANGTHSALASACGVDWHQEPYEQLAVIANVATAIPHQGRAFERFTPNGPLAMLPMSHGRCSLVWCHPLDQRDEVLSWSDA
QFCHALQAAFGWRLGRITQAGKRSAYPLALTTAAKVFTHRTVVVGNAAQTLHPIAGQGFNLGLRDVMSLAETLVAAQDAG
EDIGAWHVLSRYQQRRQVDRDTTIGVTNGLVHLFANRWAPLVIGRNIGLMAMELLPPARDALAQRTLGWVAR
>Mature_391_residues
SVIIVGGGMAGATLALAISQFSHGTLPVHLIEAKAPEADGHPGFDARAIALAAGTCQQLARIGVWQAISDCATAINTVHV
SDRGHAGFVTLDAQDYSLAALGHVAELHDIGLRLFALLRKAPGVTLHCPERVASVSRTQQQVNVTLENGNVIAGSVLVAA
NGTHSALASACGVDWHQEPYEQLAVIANVATAIPHQGRAFERFTPNGPLAMLPMSHGRCSLVWCHPLDQRDEVLSWSDAQ
FCHALQAAFGWRLGRITQAGKRSAYPLALTTAAKVFTHRTVVVGNAAQTLHPIAGQGFNLGLRDVMSLAETLVAAQDAGE
DIGAWHVLSRYQQRRQVDRDTTIGVTNGLVHLFANRWAPLVIGRNIGLMAMELLPPARDALAQRTLGWVAR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=387, Percent_Identity=26.8733850129199, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI32967307, Length=377, Percent_Identity=27.5862068965517, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=80.3571428571429, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.0254452926209, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786883, Length=375, Percent_Identity=32.2666666666667, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=425, Percent_Identity=27.0588235294118, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321694, Length=450, Percent_Identity=24.8888888888889, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19920760, Length=421, Percent_Identity=24.4655581947743, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 41769; Mature: 41638

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIVGGGMAGATLALAISQFSHGTLPVHLIEAKAPEADGHPGFDARAIALAAGTCQQL
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQAISDCATAINTVHVSDRGHAGFVTLDAQDYSLAALGHVAELHDIGLRLFALLR
HHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPERVASVSRTQQQVNVTLENGNVIAGSVLVAANGTHSALASACGVDWHQEP
HCCCCEEECHHHHHHHHHCCEEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATAIPHQGRAFERFTPNGPLAMLPMSHGRCSLVWCHPLDQRDEVLSWSDA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCH
QFCHALQAAFGWRLGRITQAGKRSAYPLALTTAAKVFTHRTVVVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHCCCCCCCCC
LGLRDVMSLAETLVAAQDAGEDIGAWHVLSRYQQRRQVDRDTTIGVTNGLVHLFANRWAP
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCE
LVIGRNIGLMAMELLPPARDALAQRTLGWVAR
EEEECCCCEEHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIVGGGMAGATLALAISQFSHGTLPVHLIEAKAPEADGHPGFDARAIALAAGTCQQL
EEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQAISDCATAINTVHVSDRGHAGFVTLDAQDYSLAALGHVAELHDIGLRLFALLR
HHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPERVASVSRTQQQVNVTLENGNVIAGSVLVAANGTHSALASACGVDWHQEP
HCCCCEEECHHHHHHHHHCCEEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATAIPHQGRAFERFTPNGPLAMLPMSHGRCSLVWCHPLDQRDEVLSWSDA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCH
QFCHALQAAFGWRLGRITQAGKRSAYPLALTTAAKVFTHRTVVVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHCCCCCCCCC
LGLRDVMSLAETLVAAQDAGEDIGAWHVLSRYQQRRQVDRDTTIGVTNGLVHLFANRWAP
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCE
LVIGRNIGLMAMELLPPARDALAQRTLGWVAR
EEEECCCCEEHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]