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The map label for this gene is ygfZ [H]

Identifier: 29143316

GI number: 29143316

Start: 3054935

End: 3055915

Strand: Direct

Name: ygfZ [H]

Synonym: t2966

Alternate gene names: 29143316

Gene position: 3054935-3055915 (Clockwise)

Preceding gene: 29143313

Following gene: 29143319

Centisome position: 63.75

GC content: 56.57

Gene sequence:

>981_bases
ATGGCTTTTATCTCCTTTCCACCACGTCATCCCTCATCGTCAGCCCGCCTGCCGCTGACGCTGATCGCGCTTGACGACTG
GGCGCTGTCGTCCATTACCGGCGTAGACAGTGAAAAGTATATTCAGGGCCAGGTCACCGCTGACGTAAGCCAGATGACTG
AACAGCAACACCTTCTCGCCGCCCACTGCGATGCTAAAGGTAAGATGTGGAGCACTCTGCGACTGTTCCGTGAGCGCGAC
GGTTTCGCCTGGATTGAACGCCGTAGCGTGCGTGAAGCGCAATTAACGGAGCTGAAAAAGTACGCCGTATTTTCCAAAGT
AGTCATCGCGCCGGATGACGAACGCGTATTGCTTGGCGTGGCCGGTTTTCAGGCCCGCGCGGCGCTGGCTAATGTCTTTA
GCGAACTGCCCAATAGTGAAAATCAGGTGGTCAGAGACGGCGCCTCCACGCTGCTATGGTTTGAACATCCTGCGGAACGA
TTCCTGCTTGTCACCGATGTCGCGACGGCCAACATGTTAACGGAGAAGTTGCATGGCGAAGCCGAACTGAATAACAGTCA
ACAGTGGCTGGCGTTGGATATTGAAGCAGGCATTCCGGTGATTGACGCGGCGAACAGCGGTCAGTTTATCCCCCAGGCGA
CTAACCTACAGGCGCTGGGCGGCATCAGCTTCAAGAAAGGCTGCTACACCGGACAGGAGATGGTCGCACGGGCAAAATTC
CGCGGCGCCAATAAACGCGCGCTTTGGTTGCTGGCGGGCAAAGCCAGCCGGGTACCGGAAGCAGGAGAAGATCTGGAGCT
ACAGATGGGCGAGAACTGGCGTCGTACCGGCGCTATTCTGGCGGCGACACAGTTGGACGATGGTCAGTTACTGGTTCAGG
CCGTCATGAATAACGATTTAGAAGCCGAAAGCGTCTTCCGCGTTCGCGACGATGCCAACACCCTGCATATCGTCCCGCTG
CCTTACTCGTTGGAAGAGTGA

Upstream 100 bases:

>100_bases
ACGATCAATCCGCTTTCAATGGGCTATTTAAAGCACTTGCAATGACCGATGGCTCTTTTACCATTAACCATTATTGTTGC
AGCTAACCAGGACATTATTT

Downstream 100 bases:

>100_bases
TATCACGTTAAAAGTCGGATAGCCTGATAGCGCAACGCCATCAGGCTCGCACGTGCTGCTCTCGTAGGCCGGATAAGGTA
CGACAGCGCCGCCATCCGGC

Product: global regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAFISFPPRHPSSSARLPLTLIALDDWALSSITGVDSEKYIQGQVTADVSQMTEQQHLLAAHCDAKGKMWSTLRLFRERD
GFAWIERRSVREAQLTELKKYAVFSKVVIAPDDERVLLGVAGFQARAALANVFSELPNSENQVVRDGASTLLWFEHPAER
FLLVTDVATANMLTEKLHGEAELNNSQQWLALDIEAGIPVIDAANSGQFIPQATNLQALGGISFKKGCYTGQEMVARAKF
RGANKRALWLLAGKASRVPEAGEDLELQMGENWRRTGAILAATQLDDGQLLVQAVMNNDLEAESVFRVRDDANTLHIVPL
PYSLEE

Sequences:

>Translated_326_residues
MAFISFPPRHPSSSARLPLTLIALDDWALSSITGVDSEKYIQGQVTADVSQMTEQQHLLAAHCDAKGKMWSTLRLFRERD
GFAWIERRSVREAQLTELKKYAVFSKVVIAPDDERVLLGVAGFQARAALANVFSELPNSENQVVRDGASTLLWFEHPAER
FLLVTDVATANMLTEKLHGEAELNNSQQWLALDIEAGIPVIDAANSGQFIPQATNLQALGGISFKKGCYTGQEMVARAKF
RGANKRALWLLAGKASRVPEAGEDLELQMGENWRRTGAILAATQLDDGQLLVQAVMNNDLEAESVFRVRDDANTLHIVPL
PYSLEE
>Mature_325_residues
AFISFPPRHPSSSARLPLTLIALDDWALSSITGVDSEKYIQGQVTADVSQMTEQQHLLAAHCDAKGKMWSTLRLFRERDG
FAWIERRSVREAQLTELKKYAVFSKVVIAPDDERVLLGVAGFQARAALANVFSELPNSENQVVRDGASTLLWFEHPAERF
LLVTDVATANMLTEKLHGEAELNNSQQWLALDIEAGIPVIDAANSGQFIPQATNLQALGGISFKKGCYTGQEMVARAKFR
GANKRALWLLAGKASRVPEAGEDLELQMGENWRRTGAILAATQLDDGQLLVQAVMNNDLEAESVFRVRDDANTLHIVPLP
YSLEE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=326, Percent_Identity=85.5828220858896, Blast_Score=579, Evalue=1e-167,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 36001; Mature: 35870

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFISFPPRHPSSSARLPLTLIALDDWALSSITGVDSEKYIQGQVTADVSQMTEQQHLLA
CCEECCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
AHCDAKGKMWSTLRLFRERDGFAWIERRSVREAQLTELKKYAVFSKVVIAPDDERVLLGV
EECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEE
AGFQARAALANVFSELPNSENQVVRDGASTLLWFEHPAERFLLVTDVATANMLTEKLHGE
CCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEECCCCEEEEEECHHHHHHHHHHHCCC
AELNNSQQWLALDIEAGIPVIDAANSGQFIPQATNLQALGGISFKKGCYTGQEMVARAKF
CCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHH
RGANKRALWLLAGKASRVPEAGEDLELQMGENWRRTGAILAATQLDDGQLLVQAVMNNDL
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCC
EAESVFRVRDDANTLHIVPLPYSLEE
CHHHEEEEECCCCEEEEEECCCCCCC
>Mature Secondary Structure 
AFISFPPRHPSSSARLPLTLIALDDWALSSITGVDSEKYIQGQVTADVSQMTEQQHLLA
CEECCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
AHCDAKGKMWSTLRLFRERDGFAWIERRSVREAQLTELKKYAVFSKVVIAPDDERVLLGV
EECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEE
AGFQARAALANVFSELPNSENQVVRDGASTLLWFEHPAERFLLVTDVATANMLTEKLHGE
CCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEECCCCEEEEEECHHHHHHHHHHHCCC
AELNNSQQWLALDIEAGIPVIDAANSGQFIPQATNLQALGGISFKKGCYTGQEMVARAKF
CCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHH
RGANKRALWLLAGKASRVPEAGEDLELQMGENWRRTGAILAATQLDDGQLLVQAVMNNDL
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCC
EAESVFRVRDDANTLHIVPLPYSLEE
CHHHEEEEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA