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The map label for this gene is recJ [H]

Identifier: 29143310

GI number: 29143310

Start: 3049966

End: 3051699

Strand: Reverse

Name: recJ [H]

Synonym: t2960

Alternate gene names: 29143310

Gene position: 3051699-3049966 (Counterclockwise)

Preceding gene: 29143311

Following gene: 29143308

Centisome position: 63.68

GC content: 57.27

Gene sequence:

>1734_bases
GTGAAACAACAGAGACAACTTCGTCGGCGCGAGGCTGATGAGACGGCGGAACTCCCCGCCGATCTTCCTCCATTACTACG
ACGTCTATATGCCAGCCGGGGCGTTCGTAGCGCCCGCGAACTGGAGCGCAGCGTGAAAGGAATGCTGCCCTGGCAACAGC
TTAGCGGCATAGATAACGCGGTGGAGATCCTCTACAACGCCTTTCGCGAAGGTACCCGCATTATTGTTGTTGGCGATTTT
GACGCCGACGGCGCGACCAGTACTGCGCTAAGCGTATTGGGAATGCGTGCGTTGGGATGTGACAACATCAGTTATCTGGT
GCCTAATCGCTTTGAGGACGGCTACGGTTTAAGCCCGGAAGTGGTCGATCAGGCGAAGGCGCGCGGCGCACAGCTTATCG
TCACCGTAGACAACGGCATTTCATCCCACGCTGGCGTAGCGCATGCAAAGACGTTGAGGATTCCGGTGATCGTGACCGAT
CACCACCTGCCTGGCGACACGTTGCCGGATGCCGAAGCGATTATTAATCCCAATCTGCGCGACTGCGAATTCCCGTCTAA
GTCGCTGGCGGGCGTCGGCGTGGCGTTTTACCTGATGCTGGCGTTGCGAACATTTTTGCGCGACAAAGGATGGTTCGACG
AGCGCGGCATCGCGCCGCCGAATCTGGCGGAGCTGCTGGATTTAGTGGCGTTGGGAACGGTAGCAGACGTTGTGCCGCTG
GACGCCAACAACCGTATTCTGACCTGGCAAGGGCTAAGTCGTATTCGTGCCGGGAAATGCCGTCCGGGAATTAAAGCGTT
GCTGGAGATATCGAATCGCGATCCGCAGCAGCTTGCCGCCAGTGATTTAGGCTTCGCGTTGGGGCCTCGTCTGAATGCCG
CCGGCAGGCTGGATGATATGTCCGTTGGCGTGGCGTTACTGTTGTGCGACAACCTCGGCGAAGCGCGTGTTCTGGCCAGC
GAGCTGGATGCGCTTAACCAGACGCGCAAAGAAATAGAGCAGGGGATGCAGGCGGAAGCGCTTATCCTGTGCGAAAAGCT
TGAGCGCAGTAGTGAAACGCTTCCGGGCGGTCTGGCGATGTATCATCCTGAATGGCATCAGGGCGTGGTCGGAATTCTGG
CGTCGCGCATTAAAGAGCGTTTTCACCGCCCGGTGATCGCCTTTGCACCTGCGGGCGACGGCACGCTAAAAGGCTCAGGC
CGTTCGATTCAGGGGTTGCATATGCGCGATGCGCTGGAAAGGCTGGATACGCTTTACCCTGATCTGATGATCAAGTTCGG
CGGTCATGCGATGGCGGCGGGATTGTCGCTGGAAGAGCATAAATTCGAGCAGTTTCAGCAACGTTTTGGCGAGCTGGTGA
CGGAATGGCTCGATCCTGCCCTGTTGCAAGGCGAGGTGATCTCCGATGGTCCATTAAGCGCGGCGGAGATGTCTATGGAA
GTGGCGCAACTGTTGCGGGATGCCGGACCGTGGGGGCAAATGTTCCCGGAACCGTTATTCGATGGTCGTTTCCGTCTGCT
ACAACAGCGTCTGGTGGGCGAGCGCCACCTCAAGGTGATGGTGGAGCCTGTCGGCGGCGGCCCGCTGTTGGATGGCATCG
CATTTAATATTGATACGACCTGTTGGCCGGATAACGGCGTGCGGGAGGTAGAACTGGCTTATAAACTGGACATTAACGAG
TTTCGCGGCAACCGTAGTTTACAGATTATTATCGATGATATTTGGCCGCTATGA

Upstream 100 bases:

>100_bases
TATTGAGTAACGGCTATGTGGTGCCTGGCTATCAGGGACCGAAAGAGATGAAAGCGTTTCTTGATGAGCATCAAAAACAG
ACCAGTGGTAAATAATACGC

Downstream 100 bases:

>100_bases
TCGGGGCGATTTAACAACACTTTCGCTGTAATTAAGGGGCGAAATCCGCTAATATTTCACCCTTATAACCGCATTTTGAC
AAGTCAAATAAAAGAAATCA

Product: ssDNA exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKQQRQLRRREADETAELPADLPPLLRRLYASRGVRSARELERSVKGMLPWQQLSGIDNAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLGMRALGCDNISYLVPNRFEDGYGLSPEVVDQAKARGAQLIVTVDNGISSHAGVAHAKTLRIPVIVTD
HHLPGDTLPDAEAIINPNLRDCEFPSKSLAGVGVAFYLMLALRTFLRDKGWFDERGIAPPNLAELLDLVALGTVADVVPL
DANNRILTWQGLSRIRAGKCRPGIKALLEISNRDPQQLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNLGEARVLAS
ELDALNQTRKEIEQGMQAEALILCEKLERSSETLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPDLMIKFGGHAMAAGLSLEEHKFEQFQQRFGELVTEWLDPALLQGEVISDGPLSAAEMSME
VAQLLRDAGPWGQMFPEPLFDGRFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNIDTTCWPDNGVREVELAYKLDINE
FRGNRSLQIIIDDIWPL

Sequences:

>Translated_577_residues
MKQQRQLRRREADETAELPADLPPLLRRLYASRGVRSARELERSVKGMLPWQQLSGIDNAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLGMRALGCDNISYLVPNRFEDGYGLSPEVVDQAKARGAQLIVTVDNGISSHAGVAHAKTLRIPVIVTD
HHLPGDTLPDAEAIINPNLRDCEFPSKSLAGVGVAFYLMLALRTFLRDKGWFDERGIAPPNLAELLDLVALGTVADVVPL
DANNRILTWQGLSRIRAGKCRPGIKALLEISNRDPQQLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNLGEARVLAS
ELDALNQTRKEIEQGMQAEALILCEKLERSSETLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPDLMIKFGGHAMAAGLSLEEHKFEQFQQRFGELVTEWLDPALLQGEVISDGPLSAAEMSME
VAQLLRDAGPWGQMFPEPLFDGRFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNIDTTCWPDNGVREVELAYKLDINE
FRGNRSLQIIIDDIWPL
>Mature_577_residues
MKQQRQLRRREADETAELPADLPPLLRRLYASRGVRSARELERSVKGMLPWQQLSGIDNAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLGMRALGCDNISYLVPNRFEDGYGLSPEVVDQAKARGAQLIVTVDNGISSHAGVAHAKTLRIPVIVTD
HHLPGDTLPDAEAIINPNLRDCEFPSKSLAGVGVAFYLMLALRTFLRDKGWFDERGIAPPNLAELLDLVALGTVADVVPL
DANNRILTWQGLSRIRAGKCRPGIKALLEISNRDPQQLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNLGEARVLAS
ELDALNQTRKEIEQGMQAEALILCEKLERSSETLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDTLYPDLMIKFGGHAMAAGLSLEEHKFEQFQQRFGELVTEWLDPALLQGEVISDGPLSAAEMSME
VAQLLRDAGPWGQMFPEPLFDGRFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNIDTTCWPDNGVREVELAYKLDINE
FRGNRSLQIIIDDIWPL

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=577, Percent_Identity=91.3344887348353, Blast_Score=1079, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63410; Mature: 63410

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQRQLRRREADETAELPADLPPLLRRLYASRGVRSARELERSVKGMLPWQQLSGIDNA
CCHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCHHH
VEILYNAFREGTRIIVVGDFDADGATSTALSVLGMRALGCDNISYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHH
VVDQAKARGAQLIVTVDNGISSHAGVAHAKTLRIPVIVTDHHLPGDTLPDAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCCCCCCHHEEEEEEEEEECCCCCCCCCCCCHHHCCCCCC
DCEFPSKSLAGVGVAFYLMLALRTFLRDKGWFDERGIAPPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEC
DANNRILTWQGLSRIRAGKCRPGIKALLEISNRDPQQLAASDLGFALGPRLNAAGRLDDM
CCCCCEEEECCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNLGEARVLASELDALNQTRKEIEQGMQAEALILCEKLERSSETLPGGLAM
HHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DLMIKFGGHAMAAGLSLEEHKFEQFQQRFGELVTEWLDPALLQGEVISDGPLSAAEMSME
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGRFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNIDTT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCCC
CWPDNGVREVELAYKLDINEFRGNRSLQIIIDDIWPL
CCCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKQQRQLRRREADETAELPADLPPLLRRLYASRGVRSARELERSVKGMLPWQQLSGIDNA
CCHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCHHH
VEILYNAFREGTRIIVVGDFDADGATSTALSVLGMRALGCDNISYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHH
VVDQAKARGAQLIVTVDNGISSHAGVAHAKTLRIPVIVTDHHLPGDTLPDAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCCCCCCHHEEEEEEEEEECCCCCCCCCCCCHHHCCCCCC
DCEFPSKSLAGVGVAFYLMLALRTFLRDKGWFDERGIAPPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEC
DANNRILTWQGLSRIRAGKCRPGIKALLEISNRDPQQLAASDLGFALGPRLNAAGRLDDM
CCCCCEEEECCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNLGEARVLASELDALNQTRKEIEQGMQAEALILCEKLERSSETLPGGLAM
HHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DLMIKFGGHAMAAGLSLEEHKFEQFQQRFGELVTEWLDPALLQGEVISDGPLSAAEMSME
HHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGRFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNIDTT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCCC
CWPDNGVREVELAYKLDINEFRGNRSLQIIIDDIWPL
CCCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]