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The map label for this gene is gcvA [H]

Identifier: 29143244

GI number: 29143244

Start: 2975705

End: 2976622

Strand: Reverse

Name: gcvA [H]

Synonym: t2890

Alternate gene names: 29143244

Gene position: 2976622-2975705 (Counterclockwise)

Preceding gene: 29143245

Following gene: 29143243

Centisome position: 62.12

GC content: 50.33

Gene sequence:

>918_bases
ATGTCAAAACGATTACCTCCTTTAAATGCGTTACGCGTTTTTGATGCTGCCGCGCGCCATTTGAGTTTTACGCGAGCAGC
GGAAGAACTTTTTGTGACGCAGGCCGCAGTAAGCCACCAAATTAAGTCACTTGAGGATTTTTTGGGGCTAAAACTGTTCC
GCCGCCGCAATCGTTCGCTGCTGCTGACGGAAGAAGGCCAAAGCTATTTTCTTGATATTAAAGAGATATTTTCCCAGCTA
ACGGAAGCAACGCGTAAGCTCCAGGCGCGCAGTGCCAAAGGGGCGCTGACGGTGAGTTTACCGCCCAGTTTTGCCATCCA
GTGGCTGGTGCCCAGGCTCTCCAGCTTTAACTCAGCTTATCCGGGAATTGACGTTCGGATACAAGCCGTTGATCGTCAGG
AAGATAAACTGGCAGACGATGTCGATGTCGCGATTTTTTATGGCCGGGGAAACTGGCCGGGCTTGCGCGTCGAAAAATTA
TACGCAGAATATTTATTGCCCGTCTGTTCTCCTTTATTGCTCACAGGCGAAAAACCGTTAAAAACGCCGGAGGATCTGGC
GAAACATACGTTATTGCACGATGCCTCCCGGCGTGACTGGCAGGCCTATACTCGTCAGTTAGGGCTCAACCATATTAACG
TTCAGCAAGGGCCGATATTTAGCCACAGCGCGATGGTGCTACAGGCGGCTATTCACGGACAGGGGATCGCGCTGGCGAAT
AATGTTATGGCGCAGTCGGAAATTGAAGCCGGGCGACTGGTGTGTCCATTTAACGATGTTCTGGTGAGTAAGAATGCTTT
TTATCTGGTTTGTCATGACAGCCAGGCAGAACTGGGTAAAATAGCCGCCTTCCGTCAGTGGATTTTGGCGAAGGCGGCGA
CGGAGCAAGAAAAATTCCGTTTTCGTTACGAACAATAA

Upstream 100 bases:

>100_bases
AACCTCAACGGACAATTTATAATGTCTCAGATTAAAAAAACTAATAGGTTACCTATTGTTACCTATTTGTTAAATTCATT
CGACATTAAGTCCAGAGGCC

Downstream 100 bases:

>100_bases
TTAACTTAGGGTATGACCATGACCAGCCGTTTTATGCTGATTGTCGCCGCTATCAGCGGTTTTATTTACGTTGCTCTGGG
CGCGTTCGGGGCGCATGTTT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQL
TEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQAYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGIALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAATEQEKFRFRYEQ

Sequences:

>Translated_305_residues
MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQL
TEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQAYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGIALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAATEQEKFRFRYEQ
>Mature_304_residues
SKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLT
EATRKLQARSAKGALTVSLPPSFAIQWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLY
AEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQAYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGIALANN
VMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAATEQEKFRFRYEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=98.3606557377049, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.965034965035, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.6122448979592, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=28.0405405405405, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=28.8732394366197, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=31.1320754716981, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=31.9066147859922, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=26.4, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=23.2786885245902, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=176, Percent_Identity=34.0909090909091, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789440, Length=266, Percent_Identity=26.6917293233083, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693105, Length=143, Percent_Identity=29.3706293706294, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=176, Percent_Identity=28.9772727272727, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=32.3076923076923, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34391; Mature: 34260

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSSFNSAY
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGEKPL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHEECCCCCC
KTPEDLAKHTLLHDASRRDWQAYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGIALAN
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCEEHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAATEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHH
FRYEQ
CCCCC
>Mature Secondary Structure 
SKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSSFNSAY
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGEKPL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHEECCCCCC
KTPEDLAKHTLLHDASRRDWQAYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGIALAN
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCEEHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAATEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHH
FRYEQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]