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The map label for this gene is cysJ [H]

Identifier: 29143204

GI number: 29143204

Start: 2925435

End: 2927234

Strand: Reverse

Name: cysJ [H]

Synonym: t2849

Alternate gene names: 29143204

Gene position: 2927234-2925435 (Counterclockwise)

Preceding gene: 29143206

Following gene: 29143203

Centisome position: 61.09

GC content: 57.67

Gene sequence:

>1800_bases
ATGACGACACCGGCTCCACTGACTGGGTTGCTTCCGCTTAATCCGGAGCAACTGGCGCGCCTTCAGGCCGCGACAACCGA
CCTTACTCCCGAACAGCTTGCCTGGGTCTCCGGCTTTTTTTGGGGCGTACTGAACCCGCGCTCAGGCGCTGTTGCCGTCA
CGCCTGCTCCTGATGGGAAGATGCCGGGAGTTACGCTTATCTCCGCTTCGCAAACCGGCAATGCGCGTCGTGTGGCGGAA
GCGCTGCGTGATGATCTGCTGGCGGCCAACCTTAATGTGACTCTGGTGAACGCCGGCGACTACAAATTCAAACAGATTGC
CAGTGAAAAACTGTTGGTGATAGTGACGTCCACCCAGGGAGAAGGCGAGCCGCCGGAAGAGGCTGTCGCGCTGCATAAGT
TCCTGTTCTCGAAAAAAGCGCCGAAACTAGAAAACACCGCTTTTGCCGTGTTTAGCCTCGGCGACACCTCTTATGAATTT
TTCTGCCAGTCCGGAAAAGATTTCGACAGCAAACTGGCGGAACTGGGCGGTGAGCGTCTGCTCGACCGTGTCGATGCTGA
CGTTGAATATCAGGCAGCTGCCAGCGAATGGCGCGCCTGCGTGGTTGATGTTCTGAAGTCCCGTGCACCTGTCGCGGTAC
CTTCGCAATCCGTCGCTACTGGCGCGGTAAACGACATCCACACCAGCCCCTATACCAAAGACGCGCCGCTGACCGCGACC
CTCTCCGTGAATCAGAAAATCACCGGTCGCAACTCGGAAAAAGACGTTCGTCATATTGAGATTGATTTAGGCGACTCCGG
TCTGCGTTACCAGCCGGGCGATGCGCTGGGCGTCTGGTATCAGAACGATCCGGCTCTGGTGAAGGAACTGGTTGAGTTGC
TGTGGCTGAAAGGCGATGAGCCGGTGATGGTTGATGGTAAAACCCTGCCGCTCGCCGAAGCGCTGGAGTGGCACTTCGAG
CTGACGGTGAATACCGCCAACATTGTTGAGAACTACGCGACCCTGACGCGCAGTGAGTCGCTGTTGCCATTAGTGGGTGA
CAAAGCGCAGCTCCAGCATTACGCCGCGACCACGCCGATCGTTGATATGGTGCGTTTCTCTCCGGCGCAGCTGGATGCTC
AGGCGCTAATCGACCTGCTGCGCCCGCTGACGCCGCGTCTGTACTCCATCGCCTCCGCACAGGCTGAAGTGGAGAGTGAA
GTGCATATCACCGTTGGCGTGGTTCGCTATGATATCGAAGGCCGCGCCCGCGCGGGTGGCGCTTCCAGCTTCCTCGCCGA
CCGCGTGGAAGAAGAGGGCGAAGTTCGCGTCTTTATTGAGCATAACGATAACTTCCGCCTGCCAGCGAACCCGCAAACGC
CGGTGATCATGATTGGCCCCGGCACCGGCATCGCGCCGTTCCGCGCCTTTATGCAACAGCGTGCCGCCGATGGCGCGGAA
GGCAAGAACTGGCTGTTCTTCGGCAACCCGCACTTTACCGAAGATTTCCTCTATCAGGTGGAGTGGCAGCGCTACGTGAA
AGAGGGACTGTTAAGCCGTATCGATCTCGCCTGGTCCCGCGATCAAAAAGAAAAAATTTACGTACAAGACAAGCTGCGTG
AACAGGGCGCGGAGCTGTGGCGCTGGATCAATGATGGCGCCCACATTTATGTCTGCGGCGACGCCAGGCGCATGGCCGCA
GATGTCGAAAAAGCATTGCTGGAAGTGATCGCTGAATTCGGCGGTATGGACCTCGAATCGGCAGATGAATATTTAAGTGA
GCTGCGCGTAGAGCGCCGTTATCAGCGAGATGTCTACTAA

Upstream 100 bases:

>100_bases
ATGTACGCTCCTGGGGCGCGATCAGCTTACAGTCTGGGCTACAGCGAACTTGAATGATTTTGTGTATAGATCCGCTTTGC
TTACTGGAACATAACGACGC

Downstream 100 bases:

>100_bases
TGAGCGAAAAATATCCAGGCCCACTGGTGGTCGAAGGTAAACTGTCCGATGCCGAGCGCATGAAGCTGGAAAGCAACTAC
CTGCGCGGTACCATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTPAPLTGLLPLNPEQLARLQAATTDLTPEQLAWVSGFFWGVLNPRSGAVAVTPAPDGKMPGVTLISASQTGNARRVAE
ALRDDLLAANLNVTLVNAGDYKFKQIASEKLLVIVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDTSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRACVVDVLKSRAPVAVPSQSVATGAVNDIHTSPYTKDAPLTAT
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVMVDGKTLPLAEALEWHFE
LTVNTANIVENYATLTRSESLLPLVGDKAQLQHYAATTPIVDMVRFSPAQLDAQALIDLLRPLTPRLYSIASAQAEVESE
VHITVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPQTPVIMIGPGTGIAPFRAFMQQRAADGAE
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGLLSRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDARRMAA
DVEKALLEVIAEFGGMDLESADEYLSELRVERRYQRDVY

Sequences:

>Translated_599_residues
MTTPAPLTGLLPLNPEQLARLQAATTDLTPEQLAWVSGFFWGVLNPRSGAVAVTPAPDGKMPGVTLISASQTGNARRVAE
ALRDDLLAANLNVTLVNAGDYKFKQIASEKLLVIVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDTSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRACVVDVLKSRAPVAVPSQSVATGAVNDIHTSPYTKDAPLTAT
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVMVDGKTLPLAEALEWHFE
LTVNTANIVENYATLTRSESLLPLVGDKAQLQHYAATTPIVDMVRFSPAQLDAQALIDLLRPLTPRLYSIASAQAEVESE
VHITVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPQTPVIMIGPGTGIAPFRAFMQQRAADGAE
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGLLSRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDARRMAA
DVEKALLEVIAEFGGMDLESADEYLSELRVERRYQRDVY
>Mature_598_residues
TTPAPLTGLLPLNPEQLARLQAATTDLTPEQLAWVSGFFWGVLNPRSGAVAVTPAPDGKMPGVTLISASQTGNARRVAEA
LRDDLLAANLNVTLVNAGDYKFKQIASEKLLVIVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDTSYEFF
CQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRACVVDVLKSRAPVAVPSQSVATGAVNDIHTSPYTKDAPLTATL
SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVMVDGKTLPLAEALEWHFEL
TVNTANIVENYATLTRSESLLPLVGDKAQLQHYAATTPIVDMVRFSPAQLDAQALIDLLRPLTPRLYSIASAQAEVESEV
HITVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPQTPVIMIGPGTGIAPFRAFMQQRAADGAEG
KNWLFFGNPHFTEDFLYQVEWQRYVKEGLLSRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDARRMAAD
VEKALLEVIAEFGGMDLESADEYLSELRVERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=601, Percent_Identity=30.6156405990017, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI7657393, Length=595, Percent_Identity=30.4201680672269, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI221316709, Length=595, Percent_Identity=30.2521008403361, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI221316705, Length=604, Percent_Identity=29.9668874172185, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=29.9832495812395, Blast_Score=220, Evalue=4e-57,
Organism=Homo sapiens, GI10835173, Length=650, Percent_Identity=29.3846153846154, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI221316707, Length=529, Percent_Identity=29.6786389413989, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI40254422, Length=646, Percent_Identity=28.328173374613, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI169790956, Length=420, Percent_Identity=28.0952380952381, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI169790958, Length=420, Percent_Identity=28.0952380952381, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=90.6510851419032, Blast_Score=1115, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=592, Percent_Identity=30.4054054054054, Blast_Score=227, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17566446, Length=585, Percent_Identity=24.957264957265, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531441, Length=395, Percent_Identity=25.3164556962025, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=641, Percent_Identity=29.1731669266771, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321143, Length=392, Percent_Identity=31.1224489795918, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6325305, Length=626, Percent_Identity=23.3226837060703, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI17137192, Length=622, Percent_Identity=28.6173633440514, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24582192, Length=511, Percent_Identity=29.7455968688845, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI78706872, Length=526, Percent_Identity=30.9885931558935, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24583543, Length=526, Percent_Identity=30.9885931558935, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI78706876, Length=526, Percent_Identity=30.9885931558935, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24660907, Length=591, Percent_Identity=25.2115059221658, Blast_Score=147, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24660903, Length=591, Percent_Identity=25.2115059221658, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66176; Mature: 66045

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPAPLTGLLPLNPEQLARLQAATTDLTPEQLAWVSGFFWGVLNPRSGAVAVTPAPDGK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCEEEEECCCCCC
MPGVTLISASQTGNARRVAEALRDDLLAANLNVTLVNAGDYKFKQIASEKLLVIVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHEEECCCEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDTSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRACVVDVLKSRAPVAVPSQSVATGAVNDIHTSPYTKDAPLTAT
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDE
EEECCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVMVDGKTLPLAEALEWHFELTVNTANIVENYATLTRSESLLPLVGDKAQLQHYAATTPI
CEEECCCCCCHHHHCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCH
VDMVRFSPAQLDAQALIDLLRPLTPRLYSIASAQAEVESEVHITVGVVRYDIEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPQTPVIMIGPGTGIAPFRAFMQQRAADGAE
HHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGLLSRIDLAWSRDQKEKIYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
RWINDGAHIYVCGDARRMAADVEKALLEVIAEFGGMDLESADEYLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTPAPLTGLLPLNPEQLARLQAATTDLTPEQLAWVSGFFWGVLNPRSGAVAVTPAPDGK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCEEEEECCCCCC
MPGVTLISASQTGNARRVAEALRDDLLAANLNVTLVNAGDYKFKQIASEKLLVIVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHEEECCCEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDTSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRACVVDVLKSRAPVAVPSQSVATGAVNDIHTSPYTKDAPLTAT
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDE
EEECCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVMVDGKTLPLAEALEWHFELTVNTANIVENYATLTRSESLLPLVGDKAQLQHYAATTPI
CEEECCCCCCHHHHCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCH
VDMVRFSPAQLDAQALIDLLRPLTPRLYSIASAQAEVESEVHITVGVVRYDIEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPQTPVIMIGPGTGIAPFRAFMQQRAADGAE
HHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGLLSRIDLAWSRDQKEKIYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
RWINDGAHIYVCGDARRMAADVEKALLEVIAEFGGMDLESADEYLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA