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The map label for this gene is truD [H]

Identifier: 29143184

GI number: 29143184

Start: 2904650

End: 2905699

Strand: Reverse

Name: truD [H]

Synonym: t2829

Alternate gene names: 29143184

Gene position: 2905699-2904650 (Counterclockwise)

Preceding gene: 29143185

Following gene: 29143183

Centisome position: 60.64

GC content: 53.81

Gene sequence:

>1050_bases
ATGACAGAGTTTGACAATCTCACCTGGCTGCACGGAAAACCACAAGGTAGCGGATTGCTGAAAGCGAATCCGGAAGATTT
TGTGGTGGTTGAAGATTTAGGCTTTACGCCGGATGGTGAAGGCGAGCACATTCTGCTGCGTATTTTGAAAAACGGTTGCA
ATACCCGTTTTGTCGCTGACGTGCTGGCGAAATTCCTGAAAATTCACGCTCGCGAAGTGAGCTTTGCCGGGCAAAAAGAT
AAACACGCCGTTACCGAGCAGTGGCTGTGCGCGCGCGTGCCGGGGAAAGAGATGCCCGATTTCAGCGCCTTTCAGCTGGA
AGGTTGTAAAGTACTGGAATACGCGCGTCACAAGCGTAAGCTGCGTTTAGGCGCGCTGAAAGGCAATGCCTTTACGCTGG
TATTGCGTGAAATAAGCGATCGCCGCGATGTCGAAACACGATTGCAGGCGATTCGCGATGGCGGCGTGCCGAACTATTTT
GGCGCACAACGATTCGGCATTGGCGGCAGCAACTTGCAGGGCGCGCTGCGCTGGGCGCAAAGTAACGCGCCGGTGCGCGA
TCGCAATAAACGCAGTTTTTGGTTGTCGGCGGCGCGTAGCGCGTTGTTTAATCAAATTGTTCACCAGCGGCTGAAGAAAC
CAGACTTTAATCAAGTTGTTGACGGCGATGCGCTACAATTAGCGGGGCGTGGAAGCTGGTTCGTCGCGACATCTGAAGAA
CTGCCTGAATTGCAGCGCCGGGTAGACGAAAAAGAGCTGATGATTACAGCGTCGTTGCCGGGCAGCGGAGAGTGGGGAAC
ACAGCGTGCGGCGCTGGCGTTTGAGCAAGATGCCATTGCCCAGGAAACGGTGTTGCAATCGCTGTTGCTGCGCGAAAAAG
TAGAGGCGTCGCGCAGAGCGATGCTGCTTTATCCGCAACAATTAAGCTGGAACTGGTGGGATGACGTTACCGTCGAGTTA
CGTTTCTGGCTGCCCGCAGGCAGTTTTGCCACCAGCGTAGTAAGGGAACTGATCAACACAATGGGTGATTATGCGCATAT
TGCTGAGTAA

Upstream 100 bases:

>100_bases
ATGTTAACGTGAAGGCGACGACGACGGAAAAGCTCGGATTTACCGGACGCGGAGAAGGCATTGCCTGCGAAGCGGTGGCG
TTGCTGATGAAGGCGGCGAA

Downstream 100 bases:

>100_bases
CGATGACGGGGTTCACGCGCCCGGTATACAAACGCTGGCGAAAGCGCTGCGTGAGTTTGCTGATGTACAGGTCGTAGCCC
CGGATCGTAACCGCAGCGGC

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVADVLAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRKLRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYF
GAQRFGIGGSNLQGALRWAQSNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTMGDYAHIAE

Sequences:

>Translated_349_residues
MTEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVADVLAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRKLRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYF
GAQRFGIGGSNLQGALRWAQSNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTMGDYAHIAE
>Mature_348_residues
TEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVADVLAKFLKIHAREVSFAGQKDK
HAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRKLRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYFG
AQRFGIGGSNLQGALRWAQSNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEEL
PELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRAMLLYPQQLSWNWWDDVTVELR
FWLPAGSFATSVVRELINTMGDYAHIAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI50727002, Length=132, Percent_Identity=31.0606060606061, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789102, Length=349, Percent_Identity=88.8252148997135, Blast_Score=640, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324817, Length=144, Percent_Identity=31.9444444444444, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39361; Mature: 39230

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVAD
CCCCCCEEEECCCCCCCCCEECCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHH
VLAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHH
LRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYFGAQRFGIGGSNLQGALRWAQ
EEEEEECCCEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHC
SNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCEEEECCHH
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRA
HHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
MLLYPQQLSWNWWDDVTVELRFWLPAGSFATSVVRELINTMGDYAHIAE
EEEECHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVAD
CCCCCEEEECCCCCCCCCEECCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHH
VLAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHH
LRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYFGAQRFGIGGSNLQGALRWAQ
EEEEEECCCEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHC
SNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCEEEECCHH
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRA
HHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
MLLYPQQLSWNWWDDVTVELRFWLPAGSFATSVVRELINTMGDYAHIAE
EEEECHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA