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The map label for this gene is pphB [H]

Identifier: 29143164

GI number: 29143164

Start: 2885097

End: 2885753

Strand: Direct

Name: pphB [H]

Synonym: t2807

Alternate gene names: 29143164

Gene position: 2885097-2885753 (Clockwise)

Preceding gene: 29143163

Following gene: 29143167

Centisome position: 60.21

GC content: 41.4

Gene sequence:

>657_bases
ATGGAATTAATTCGTTATGCTGACATCAACAGCGTTCTCTATCGCCACATTTGGGTCGTTGGTGATATTCATGGATGTTA
CTCCTTATTGTTAACCAGACTGGCTCAATTAAATTTCTCTCCTGATACCGATCTCTTGATCTCCACTGGAGATAACATTG
ATCGAGGAAAAGAAAACCTTGAAACACTGAGACTGTTAAATACCTCATGGTTTATTTCTGTAGTGGGTAATCATGAAGCT
ATGGCGCTTGACGCTTTTGAAACACAGGATGGCAATTTCTGGTATGTAAACGGTGGTTACTGGTACGACTCGGTCACCCA
AAAAGACAGGCAAGAAGCCACTGAGCTATTACTAACTTTTAAGCAGCGTCCTCACATCATAGAGGTTAAAACGTCCTCAA
AGAAGTACGTGATTGCCCATGCCGATTATCCAGATGACAGCTACGATTATGGGAAGCAAGTTGATATTGATAGCGTTCTA
TGGTCACGCGATCGTCTGCTTAGTTCGTTACAGGGGAATATCCATCCAATACGCGGTGCGGATACTTTCATCTTCGGCCA
TATGATTGTTGATTACACAACAACATTCGCCAATCAAATCTACATTGACACCGGTTCTTTCTGTTCAGGCAATCTGTCTT
TTTTTAAAATAAAGTAG

Upstream 100 bases:

>100_bases
TAGGCTATTTTAATAACTGTGTATTTTAAACAATAAAAATTAATATTACCCTACTTCTTTGTTATAATACCAGCCTCCAA
AATTGTGCTATAGGAACATC

Downstream 100 bases:

>100_bases
TCTGGAAATAGAATTATATAATATTAACCCTCCCTGCATTATGGCAATGATATAGCCTTCAATAAGTACCATTGCTCATT
CGTAATAGCGTCTAAATTTC

Product: serine/threonine-specific protein phosphatase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MELIRYADINSVLYRHIWVVGDIHGCYSLLLTRLAQLNFSPDTDLLISTGDNIDRGKENLETLRLLNTSWFISVVGNHEA
MALDAFETQDGNFWYVNGGYWYDSVTQKDRQEATELLLTFKQRPHIIEVKTSSKKYVIAHADYPDDSYDYGKQVDIDSVL
WSRDRLLSSLQGNIHPIRGADTFIFGHMIVDYTTTFANQIYIDTGSFCSGNLSFFKIK

Sequences:

>Translated_218_residues
MELIRYADINSVLYRHIWVVGDIHGCYSLLLTRLAQLNFSPDTDLLISTGDNIDRGKENLETLRLLNTSWFISVVGNHEA
MALDAFETQDGNFWYVNGGYWYDSVTQKDRQEATELLLTFKQRPHIIEVKTSSKKYVIAHADYPDDSYDYGKQVDIDSVL
WSRDRLLSSLQGNIHPIRGADTFIFGHMIVDYTTTFANQIYIDTGSFCSGNLSFFKIK
>Mature_218_residues
MELIRYADINSVLYRHIWVVGDIHGCYSLLLTRLAQLNFSPDTDLLISTGDNIDRGKENLETLRLLNTSWFISVVGNHEA
MALDAFETQDGNFWYVNGGYWYDSVTQKDRQEATELLLTFKQRPHIIEVKTSSKKYVIAHADYPDDSYDYGKQVDIDSVL
WSRDRLLSSLQGNIHPIRGADTFIFGHMIVDYTTTFANQIYIDTGSFCSGNLSFFKIK

Specific function: Can hydrolyze phosphorylated Ser-, Thr- or Tyr- substrates in vitro. The natural substrate is unknown [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789090, Length=218, Percent_Identity=59.6330275229358, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI87081988, Length=213, Percent_Identity=43.1924882629108, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25027; Mature: 25027

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIRYADINSVLYRHIWVVGDIHGCYSLLLTRLAQLNFSPDTDLLISTGDNIDRGKENL
CCEEEECCHHHHHHHHHEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHH
ETLRLLNTSWFISVVGNHEAMALDAFETQDGNFWYVNGGYWYDSVTQKDRQEATELLLTF
HHHHHCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCEEECCCCHHHHHHHHHHHHHH
KQRPHIIEVKTSSKKYVIAHADYPDDSYDYGKQVDIDSVLWSRDRLLSSLQGNIHPIRGA
CCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
DTFIFGHMIVDYTTTFANQIYIDTGSFCSGNLSFFKIK
CEEEEHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEC
>Mature Secondary Structure
MELIRYADINSVLYRHIWVVGDIHGCYSLLLTRLAQLNFSPDTDLLISTGDNIDRGKENL
CCEEEECCHHHHHHHHHEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHH
ETLRLLNTSWFISVVGNHEAMALDAFETQDGNFWYVNGGYWYDSVTQKDRQEATELLLTF
HHHHHCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCEEECCCCHHHHHHHHHHHHHH
KQRPHIIEVKTSSKKYVIAHADYPDDSYDYGKQVDIDSVLWSRDRLLSSLQGNIHPIRGA
CCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
DTFIFGHMIVDYTTTFANQIYIDTGSFCSGNLSFFKIK
CEEEEHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11717262; 11677609 [H]