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The map label for this gene is spaS [H]

Identifier: 29143148

GI number: 29143148

Start: 2869390

End: 2870460

Strand: Reverse

Name: spaS [H]

Synonym: t2789

Alternate gene names: 29143148

Gene position: 2870460-2869390 (Counterclockwise)

Preceding gene: 29143149

Following gene: 29143147

Centisome position: 59.9

GC content: 43.14

Gene sequence:

>1071_bases
ATGTCCTCGAATAAAACAGAAAAACCGACTAAAAAACGGCTGGAAGACTCCGCTAAAAAAGGCCAGTCATTTAAAAGTAA
AGATCTCATTATCGCCTGTCTGACGCTGGGAGGAATTGCCTATCTGGTGTCGTATGGCTCATTTAATGAGTTTATGGGGA
TAATTAAGATCATTATTGCGGATAATTTTGATCAGAGCATGGCTGATTACAGTTTGGCCGTTTTTGGGATAGGATTAAAA
TATCTGATTCCATTTATGCTGCTCTGTTTAGTGTGTTCCGCATTACCGGCGTTATTACAGGCCGGTTTCGTGCTGGCGAC
AGAAGCATTAAAACCTAATTTATCGGCGTTAAACCCGGTAGAAGGGGCGAAAAAACTTTTTAGTATGCGCACGGTTAAAG
ATACGGTCAAAACCCTACTGTATCTCTCATCCTTTGTGGTGGCCGCCATCATTTGCTGGAAGAAATATAAGGTTGAAATC
TTTTCTCAGTTAAATGGCAATGTTGTGGATATTGCCGTCATTTGGCGTGAACTTCTCCTCGCATTGGTATTAACTTGCCT
TGCTTGCGCATTGATTGTCTTATTATTGGATGCTATTGCGGAATATTTCCTGACCATGAAAGATATGAAAATGGATAAGG
AAGAAGTGAAGCGTGAAATGAAGGAGCAGGAAGGGAACCCAGAGGTTAAATCTAAAAGACGTGAAGTTCATATGGAAATT
CTGTCTGAACAGGTGAAATCCGATATTGAAAACTCACGCCTGATTGTTGCCAACCCCACGCATATTACGATCGGGATTTA
TTTTAAACCCGAATTGATGCCGATTCCGATGATCTCGGTGTATGAAACGAATCAGCGCGCACTGGCCGTCCGCGCCTATG
CGGAGAAGGTTGGCGTACCTGTGATCGTCGATATCAAACTGGCGCGCAGTCTTTTCAAAACCCATCGCCGTTATGATCTG
GTGAGTCTGGAAGAAATTGATGAAGTTTTACGTCTTCTGGTTTGGCTGGAAGAGGTAGAAAACGCGGGCAAAGACGTTAT
TCAGCCACAAGAAAACGAGGTACGGCATTGA

Upstream 100 bases:

>100_bases
ATGCTGCTTTATTTCTCTCCGGTACTACCGGACAATGTACCGCGACTCTCTTTCCAGGCCACAGGGTTAAGCAGTTGGTT
TTACGAGCGAGGGGCGACGC

Downstream 100 bases:

>100_bases
GCCGCGTAAAGCAGTAGCGATGTATTCATTGGGAGTTTTTTGAATGTTCACTAACCACCGTCGGGGTTTAATAACTGCAT
CAGATAAACGCAGTCGTTAA

Product: surface presentation of antigens protein SpaS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIADNFDQSMADYSLAVFGIGLK
YLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEI
FSQLNGNVVDIAVIWRELLLALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVPVIVDIKLARSLFKTHRRYDL
VSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH

Sequences:

>Translated_356_residues
MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIADNFDQSMADYSLAVFGIGLK
YLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEI
FSQLNGNVVDIAVIWRELLLALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVPVIVDIKLARSLFKTHRRYDL
VSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH
>Mature_355_residues
SSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIADNFDQSMADYSLAVFGIGLKY
LIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIF
SQLNGNVVDIAVIWRELLLALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEIL
SEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVPVIVDIKLARSLFKTHRRYDLV
SLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=377, Percent_Identity=23.0769230769231, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 40138; Mature: 40007

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIA
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DNFDQSMADYSLAVFGIGLKYLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
EGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIFSQLNGNVVDIAVIWRELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
ALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVP
HHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCC
VIVDIKLARSLFKTHRRYDLVSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH
EEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIA
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DNFDQSMADYSLAVFGIGLKYLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
EGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIFSQLNGNVVDIAVIWRELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
ALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVP
HHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCC
VIVDIKLARSLFKTHRRYDLVSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH
EEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8404849; 11677609 [H]