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The map label for this gene is sprA

Identifier: 29143128

GI number: 29143128

Start: 2849967

End: 2850854

Strand: Reverse

Name: sprA

Synonym: t2769

Alternate gene names: 29143128

Gene position: 2850854-2849967 (Counterclockwise)

Preceding gene: 29143129

Following gene: 29143127

Centisome position: 59.49

GC content: 36.37

Gene sequence:

>888_bases
ATGGTATTGCCTTCAATGAATAAATCAGTTGAGGCCATTAGCAATAATCACCTTCAACAACCGAACAAATTTCCATTAAT
AAATGGATTAGCTGACGTAAGAGACTATTATGTCGCAAACTGCTTATTGTTTAAACTTAATAAAGGCAGTTTGCGAATTG
AAAACGAATTTGGGGAGTTCATCGAACAATCTGCGCTGTGTTTATTTTTATTGGAAAAAGATCAAACAATCACGCTTAGT
ATGAGCGAAATAGAAGGACATATTGATTTTTCTTCACTGGAAGTTTCCTATGACTTAATGCAAAAATTCTACAAAGTTTT
TTACAGTACAAGAAACTATAATGATCGAGAGCTATCATTAAAAACGAAACCAAAGTATTTTTTTCATGCGGACTTGTTGC
CAGGGATGAGTGATACTTTTGACTCTATTTTGCATGGTGTCGCATGTCCACGGGTTTGTAGTAATGTGAGTATTGATGAT
CATGATTATTCATATTTCTCATTGATGTATCTTATATCGGCATTTGTACGTAAGCCCGGTGGGTTTGATTTCCTTGAGCG
AGCAATAAAAATTACAACAAAAGAGAAAGTTTATAACATTATTATCAGCGATCTCACCCGCAAATGGTCACAGGCTGAGG
TGGCAGGAAAGCTGTTTATGAGCGTATCAAGTCTGAAGCGAAAACTGGCCGCTGAAGAGGTGAGTTTTAGCAAAATATAC
TTGGATGCTCGTATGAATCAGGCTATAAAATTATTACGTATGGGTGCTGGAAATATTTCACAAGTCGCGACGATGTGTGG
CTATGATACGCCTTCTTATTTTATCGCTATTTTTAAACGGCATTTTAAGATTACACCGCTTAGCTTTATGCGTACAATGA
ACCATTGA

Upstream 100 bases:

>100_bases
AATAATAAAAAAATCGGATTTAAATCATCTCTGTGAGTTTTAATTATTTGTTTATAAGAATATAACGATTTTGAGTTCCT
TATAGCACACAGGATAAAAT

Downstream 100 bases:

>100_bases
TTTGTGAAATTATTTTAAACGTTACTATCTGTTTGCGTTACAGATACCAAATATTTTTTTAATCGTAGTGTATATTTCAG
TATCGCTACACCAGTGATAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MVLPSMNKSVEAISNNHLQQPNKFPLINGLADVRDYYVANCLLFKLNKGSLRIENEFGEFIEQSALCLFLLEKDQTITLS
MSEIEGHIDFSSLEVSYDLMQKFYKVFYSTRNYNDRELSLKTKPKYFFHADLLPGMSDTFDSILHGVACPRVCSNVSIDD
HDYSYFSLMYLISAFVRKPGGFDFLERAIKITTKEKVYNIIISDLTRKWSQAEVAGKLFMSVSSLKRKLAAEEVSFSKIY
LDARMNQAIKLLRMGAGNISQVATMCGYDTPSYFIAIFKRHFKITPLSFMRTMNH

Sequences:

>Translated_295_residues
MVLPSMNKSVEAISNNHLQQPNKFPLINGLADVRDYYVANCLLFKLNKGSLRIENEFGEFIEQSALCLFLLEKDQTITLS
MSEIEGHIDFSSLEVSYDLMQKFYKVFYSTRNYNDRELSLKTKPKYFFHADLLPGMSDTFDSILHGVACPRVCSNVSIDD
HDYSYFSLMYLISAFVRKPGGFDFLERAIKITTKEKVYNIIISDLTRKWSQAEVAGKLFMSVSSLKRKLAAEEVSFSKIY
LDARMNQAIKLLRMGAGNISQVATMCGYDTPSYFIAIFKRHFKITPLSFMRTMNH
>Mature_295_residues
MVLPSMNKSVEAISNNHLQQPNKFPLINGLADVRDYYVANCLLFKLNKGSLRIENEFGEFIEQSALCLFLLEKDQTITLS
MSEIEGHIDFSSLEVSYDLMQKFYKVFYSTRNYNDRELSLKTKPKYFFHADLLPGMSDTFDSILHGVACPRVCSNVSIDD
HDYSYFSLMYLISAFVRKPGGFDFLERAIKITTKEKVYNIIISDLTRKWSQAEVAGKLFMSVSSLKRKLAAEEVSFSKIY
LDARMNQAIKLLRMGAGNISQVATMCGYDTPSYFIAIFKRHFKITPLSFMRTMNH

Specific function: Positive regulator of the expression of the invasion- associated type III secretion system encoded within SPI-1 (pathogenicity island 1)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790557, Length=123, Percent_Identity=36.5853658536585, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787776, Length=128, Percent_Identity=34.375, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789933, Length=119, Percent_Identity=31.9327731092437, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786778, Length=123, Percent_Identity=36.5853658536585, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786758, Length=91, Percent_Identity=40.6593406593407, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789932, Length=178, Percent_Identity=30.8988764044944, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786776, Length=91, Percent_Identity=37.3626373626374, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SIRC_SALTI (Q8Z4A6)

Other databases:

- EMBL:   AL627276
- EMBL:   AE014613
- RefSeq:   NP_457261.1
- RefSeq:   NP_806470.1
- ProteinModelPortal:   Q8Z4A6
- GeneID:   1068906
- GeneID:   1249296
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t2769
- KEGG:   sty:STY2988
- HOGENOM:   HBG673163
- OMA:   DFIHRIS
- ProtClustDB:   PRK15044
- BioCyc:   SENT209261:T2769-MONOMER
- BioCyc:   SENT220341:STY2988-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 33872; Mature: 33872

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPSMNKSVEAISNNHLQQPNKFPLINGLADVRDYYVANCLLFKLNKGSLRIENEFGEF
CCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEEHHHHHH
IEQSALCLFLLEKDQTITLSMSEIEGHIDFSSLEVSYDLMQKFYKVFYSTRNYNDRELSL
HHHCCEEEEEEECCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KTKPKYFFHADLLPGMSDTFDSILHGVACPRVCSNVSIDDHDYSYFSLMYLISAFVRKPG
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
GFDFLERAIKITTKEKVYNIIISDLTRKWSQAEVAGKLFMSVSSLKRKLAAEEVSFSKIY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDARMNQAIKLLRMGAGNISQVATMCGYDTPSYFIAIFKRHFKITPLSFMRTMNH
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MVLPSMNKSVEAISNNHLQQPNKFPLINGLADVRDYYVANCLLFKLNKGSLRIENEFGEF
CCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEEHHHHHH
IEQSALCLFLLEKDQTITLSMSEIEGHIDFSSLEVSYDLMQKFYKVFYSTRNYNDRELSL
HHHCCEEEEEEECCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KTKPKYFFHADLLPGMSDTFDSILHGVACPRVCSNVSIDDHDYSYFSLMYLISAFVRKPG
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
GFDFLERAIKITTKEKVYNIIISDLTRKWSQAEVAGKLFMSVSSLKRKLAAEEVSFSKIY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDARMNQAIKLLRMGAGNISQVATMCGYDTPSYFIAIFKRHFKITPLSFMRTMNH
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504