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The map label for this gene is fhlA [H]

Identifier: 29143120

GI number: 29143120

Start: 2842453

End: 2844531

Strand: Direct

Name: fhlA [H]

Synonym: t2761

Alternate gene names: 29143120

Gene position: 2842453-2844531 (Clockwise)

Preceding gene: 29143119

Following gene: 29143122

Centisome position: 59.32

GC content: 54.74

Gene sequence:

>2079_bases
ATGTCGTATACACCCATGAGCGATCTTGGACAGCAAGGCTTGTTCGATATCACTCGTACCTTATTGCAGCAGCCCGATTT
GGCGTCGCTCAGTGAAGCGCTTTCGCAACTGGTAAAGCGTTCAGCGCTCGCCGACAGCGCCGGCATTGTGCTATGGCAGG
CGCAGTCGCAACGCGCACAATATTATGCGACGCGAGAAAATGGCCGTCCTGTTGAATATGAAGATGAAACCGTACTGGCG
CACGGCCCGGTACGTCGCATCCTTTCTCGCCCCGATGCATTGCACTGTAATTTCCATGAGTTTACCGAAACCTGGCCGCA
GCTTGCCGCGAGCGGCCTCTACCCTGAATTTGGTCATTACTGTCTGCTTCCTTTAGCCGCGGAGGGCCGTATTTTCGGCG
GCTGCGAATTCATCCGTCAGGAAGATCGCCCCTGGAGTGAAAAGGAATATGACCGTCTGCATACGTTTACCCAAATTGTC
GGCGTGGTCGCCGAGCAAATTCAAAACCGGGTAAACAATAATGTCGATTATGATCTGTTGTGCCGCGAACGCGATAACTT
CCGTATTCTGGTTGCGATTACCAACGCCGTCCTGTCGCGTCTGGATATTAATGAGCTGGTAAGCGAAGTCGCAAAAGAGA
TCCACCACTATTTCAATATCGACGCGATCAGCATCGTGTTGCGCAGCCATCGCAAAAATAAGCTGAATATCTACTCCACT
CACTACCTTGATGAGCATCATCCGGCGCACGAACAGAGCGAAGTCGACGAAGCCGGCACGCTCACGGAGCGCGTCTTTAA
AAGTAAAGAGATGCTGCTTATCAACCTCAACGAACGCGATCCGCTTGCCCCCTACGAACGAATGCTGTTCGATACCTGGG
GCAATCAGATCCAGACGCTTTGCCTGCTGCCGTTGATGTCCGGCAAGACGATGCTTGGCGTGCTCAAACTGGCCCAGTGC
GAAGAAAAAGTGTTTACCACCGCCAACCTCAAGCTGCTACGTCAGATTGCCGAACGGGTAGCCATTGCCGTGGATAATGC
GCTGGCCTATCAGGAAATCCACCGTCTGAAGGAACGCCTGGTCGACGAAAATCTGGCGCTCACGGAGCAGCTTAATAACG
TCGACAGCGAGTTTGGCGAGATCATCGGGCGCAGCGAAGCGATGTACAACGTGCTAAAGCAGGTTGAGATGGTGGCGCAG
AGCGACAGTACCGTGCTGATTCTGGGCGAAACGGGAACCAGTAAAGAGTTGATTGCCCGCGCCATTCACAACCTGAGCGG
ACGTAGCGGGCGCAGAATGGTAAAGATGAACTGCGCCGCGATGCCGGCGGGGCTGCTGGAAAGCGATCTGTTTGGTCATG
AGCGCGGCGCGTTTACCGGCGCCAGCGCGCAGCGGATTGGCCGTTTCGAGCTGGCGGATAAAAGCTCGCTGTTTCTGGAT
GAAGTGGGCGACATGCCGCTGGAGCTACAGCCTAAGCTGCTGCGCGTCCTTCAGGAGCAAGAGTTTGAACGTCTCGGCAG
CAATAAACTGATCCAGACCGACGTGCGGCTGATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGATCGCGAAT
TCCATAACGATCTCTACTATCGGCTGAATGTTTTTCCGATTCAGCTTCCTCCGTTACGCGAACGTCCGGAAGATATTCCA
CTGCTGGTCAAAGCGTTTACCTTCAAAATCGCCCGCCGAATGGGGCGTAATATCGACAGCATTCCCGCCGAAACGCTGCG
CACGCTCAGCGGTATGGAATGGCCGGGGAACGTTCGCGAACTGGAGAATGTCGTCGAGCGCGCCGTGCTGCTGACGCGCG
GCAATGTGCTACAACTGTCGCTGCCGGATATGACTGCCGTAACGCCCGATACCTCGCCTGTTGCGACTGAAAGCGCCAAA
GAAGGCGAAGATGAATATCAGCTTATTATACGCGTGCTGAAAGAGACCAATGGCGTAGTGGCTGGACCAAAAGGCGCGGC
GCAGCGTCTGGGGCTTAAACGAACCCCCCTGCTGTCGCGTATGAAGCGATTGGGTATTGATAAAGACGCGTTAGCGTAA

Upstream 100 bases:

>100_bases
GCGAGTTTGCGGTCAGCCATCCGGCAATAACTTACGACTTATACCTATAATTCATTTACCGTTTTCGCACCGTTTTGCGG
TATTTTCCTGGAAAAGCAAC

Downstream 100 bases:

>100_bases
CGGGTTGCCGGATCTGATCCGGCCTGGAGATGATGCGCGCCGTAGGCCTGGCGCTTCCCCTTCACTTCTTGCGCTGATAT
TTTTCCGGCAATTCTGGAAC

Product: formate hydrogenlyase system transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQYYATRENGRPVEYEDETVLA
HGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHYCLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIV
GVVAEQIQNRVNNNVDYDLLCRERDNFRILVAITNAVLSRLDINELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTLCLLPLMSGKTMLGVLKLAQC
EEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQ
SDSTVLILGETGTSKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFHNDLYYRLNVFPIQLPPLRERPEDIP
LLVKAFTFKIARRMGRNIDSIPAETLRTLSGMEWPGNVRELENVVERAVLLTRGNVLQLSLPDMTAVTPDTSPVATESAK
EGEDEYQLIIRVLKETNGVVAGPKGAAQRLGLKRTPLLSRMKRLGIDKDALA

Sequences:

>Translated_692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQYYATRENGRPVEYEDETVLA
HGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHYCLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIV
GVVAEQIQNRVNNNVDYDLLCRERDNFRILVAITNAVLSRLDINELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTLCLLPLMSGKTMLGVLKLAQC
EEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQ
SDSTVLILGETGTSKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFHNDLYYRLNVFPIQLPPLRERPEDIP
LLVKAFTFKIARRMGRNIDSIPAETLRTLSGMEWPGNVRELENVVERAVLLTRGNVLQLSLPDMTAVTPDTSPVATESAK
EGEDEYQLIIRVLKETNGVVAGPKGAAQRLGLKRTPLLSRMKRLGIDKDALA
>Mature_691_residues
SYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQYYATRENGRPVEYEDETVLAH
GPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHYCLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIVG
VVAEQIQNRVNNNVDYDLLCRERDNFRILVAITNAVLSRLDINELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYSTH
YLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTLCLLPLMSGKTMLGVLKLAQCE
EKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQS
DSTVLILGETGTSKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFHNDLYYRLNVFPIQLPPLRERPEDIPL
LVKAFTFKIARRMGRNIDSIPAETLRTLSGMEWPGNVRELENVVERAVLLTRGNVLQLSLPDMTAVTPDTSPVATESAKE
GEDEYQLIIRVLKETNGVVAGPKGAAQRLGLKRTPLLSRMKRLGIDKDALA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=691, Percent_Identity=89.8697539797395, Blast_Score=1281, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=569, Percent_Identity=49.9121265377856, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=45.9375, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1790437, Length=316, Percent_Identity=46.5189873417722, Blast_Score=265, Evalue=7e-72,
Organism=Escherichia coli, GI1788905, Length=310, Percent_Identity=44.1935483870968, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87082152, Length=375, Percent_Identity=37.0666666666667, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1789233, Length=244, Percent_Identity=46.3114754098361, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1790299, Length=365, Percent_Identity=38.9041095890411, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=47.4137931034483, Blast_Score=202, Evalue=7e-53,
Organism=Escherichia coli, GI1786524, Length=325, Percent_Identity=39.0769230769231, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=34.9544072948328, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=31.5950920245399, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=35.8267716535433, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78248; Mature: 78116

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQ
CCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
YYATRENGRPVEYEDETVLAHGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHY
EEEECCCCCCCCCCCCEEECCCHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCE
CLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIVGVVAEQIQNRVNNNVDYDLL
EEEEECCCCCEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
CRERDNFRILVAITNAVLSRLDINELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEE
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGKTMLGVLKLAQCEEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQSDSTVLILGETGTSKELIAR
HCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
AIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFHNDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHCCEEE
RLNVFPIQLPPLRERPEDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLSGMEWPGNVRE
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHH
LENVVERAVLLTRGNVLQLSLPDMTAVTPDTSPVATESAKEGEDEYQLIIRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTPLLSRMKRLGIDKDALA
ECCCHHHHHCCCCCCHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQ
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
YYATRENGRPVEYEDETVLAHGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHY
EEEECCCCCCCCCCCCEEECCCHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCE
CLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIVGVVAEQIQNRVNNNVDYDLL
EEEEECCCCCEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
CRERDNFRILVAITNAVLSRLDINELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEE
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGKTMLGVLKLAQCEEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQSDSTVLILGETGTSKELIAR
HCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
AIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFHNDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHCCEEE
RLNVFPIQLPPLRERPEDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLSGMEWPGNVRE
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHH
LENVVERAVLLTRGNVLQLSLPDMTAVTPDTSPVATESAKEGEDEYQLIIRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTPLLSRMKRLGIDKDALA
ECCCHHHHHCCCCCCHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]