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The map label for this gene is hycB [H]

Identifier: 29143113

GI number: 29143113

Start: 2837120

End: 2837728

Strand: Reverse

Name: hycB [H]

Synonym: t2754

Alternate gene names: 29143113

Gene position: 2837728-2837120 (Counterclockwise)

Preceding gene: 29143114

Following gene: 29143112

Centisome position: 59.22

GC content: 58.29

Gene sequence:

>609_bases
GTGAATCGTTTTGTAATTGCTGACTCCACTCTCTGTATCGGCTGCCACACCTGTGAGGCCGCCTGTTCAGAGACGCATCG
CCAGCATGGCCTGCAGTCAATGCCGCGCCTGAAAGTGATGCTGAATGAAAAAGAATCTGCGCCGCAGCTCTGTCACCACT
GTGAAGATGCGCCATGCGCCACGGTATGCCCGGTTAACGCGATTAATCGCGTTGACGGCGCTGTCCAGCTTAATGAAAGC
CTGTGCGTGAGCTGCAAACTGTGCGGTATTGCCTGTCCGTTTGGCGCTATTGAGTTTTCCGGCAGCCGTCCGCTGCATAT
TCCGGCGAACGCCAATACGCCGAAAGCGCCGCCCGCGCCGCCAGCGCCTGCGCGCGTCAGTACGTTGCTCGACTGGGTGC
CGGGCGTGCGCGCTATCGCGGTGAAATGCGACCTGTGCAGCTTTGACGAGCAAGGCCCGGCCTGTGTGCGGATGTGCCCG
ACTAAAGCGCTACACCTGGTGGAGAACACGGATATCGCCCGCGCCAGCAAACGTAAACGTGAGCTGACGTTTAATACCGA
TTTTGGCGATCTCACCTTGTTTCAGCAGGCTCAGAGTGGGGATGCATAA

Upstream 100 bases:

>100_bases
TGTCATGAATGTTTTGGGGATGGGCGTGTCGATAAGTGTCGAAAATGACATTTCAACGGCATGTTTTCGTCAAAAATGAC
AATCACCTGAGGAATGCCTG

Downstream 100 bases:

>100_bases
TGAGTTCACTTTCACTGATTACTAGCGGCGTCGCGTGGTTTGCCGCCGCCGCCGTTCTGGCGTTTCTGTTTTCGTTTCAT
AAAGCGCTGAGCGGCTGGAT

Product: formate hydrogenlyase subunit 2

Products: Oxidized ferredoxin; CO2; H2 [C]

Alternate protein names: FHL subunit 2; Hydrogenase-3 component B [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLKVMLNEKESAPQLCHHCEDAPCATVCPVNAINRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLHIPANANTPKAPPAPPAPARVSTLLDWVPGVRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVENTDIARASKRKRELTFNTDFGDLTLFQQAQSGDA

Sequences:

>Translated_202_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLKVMLNEKESAPQLCHHCEDAPCATVCPVNAINRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLHIPANANTPKAPPAPPAPARVSTLLDWVPGVRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVENTDIARASKRKRELTFNTDFGDLTLFQQAQSGDA
>Mature_202_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLKVMLNEKESAPQLCHHCEDAPCATVCPVNAINRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLHIPANANTPKAPPAPPAPARVSTLLDWVPGVRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVENTDIARASKRKRELTFNTDFGDLTLFQQAQSGDA

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789079, Length=202, Percent_Identity=96.039603960396, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI87082114, Length=186, Percent_Identity=51.6129032258064, Blast_Score=189, Evalue=8e-50,
Organism=Escherichia coli, GI1788811, Length=185, Percent_Identity=39.4594594594595, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI2367245, Length=187, Percent_Identity=37.9679144385027, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=38.9473684210526, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87082180, Length=188, Percent_Identity=35.6382978723404, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI87082179, Length=184, Percent_Identity=35.8695652173913, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI2367345, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787122, Length=114, Percent_Identity=32.4561403508772, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787872, Length=114, Percent_Identity=31.5789473684211, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI226510944, Length=172, Percent_Identity=29.0697674418605, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 21712; Mature: 21712

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
9.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLKVMLNEKESAPQLCHHCEDAPCA
CCEEEEECCEEEEECCHHHHHHHHHHHHHCHHHCCCEEEEECCCCCHHHHHHHCCCCCCC
TVCPVNAINRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLHIPANANTPKAPPAP
EECCCHHHHHCCCEEEECCCCEEEEEECEEECCCCCEEECCCCCEEECCCCCCCCCCCCC
PAPARVSTLLDWVPGVRAIAVKCDLCSFDEQGPACVRMCPTKALHLVENTDIARASKRKR
CCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCHHHHHHHCCCCHHHHHHHCC
ELTFNTDFGDLTLFQQAQSGDA
EEEEECCCCCCCEEHHHCCCCC
>Mature Secondary Structure
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLKVMLNEKESAPQLCHHCEDAPCA
CCEEEEECCEEEEECCHHHHHHHHHHHHHCHHHCCCEEEEECCCCCHHHHHHHCCCCCCC
TVCPVNAINRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLHIPANANTPKAPPAP
EECCCHHHHHCCCEEEECCCCEEEEEECEEECCCCCEEECCCCCEEECCCCCCCCCCCCC
PAPARVSTLLDWVPGVRAIAVKCDLCSFDEQGPACVRMCPTKALHLVENTDIARASKRKR
CCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCHHHHHHHCCCCHHHHHHHCC
ELTFNTDFGDLTLFQQAQSGDA
EEEEECCCCCCCEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; H+; formate [C]

Specific reaction: 2 reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 formate = CO2 + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]