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The map label for this gene is hycI [H]

Identifier: 29143106

GI number: 29143106

Start: 2830452

End: 2830922

Strand: Reverse

Name: hycI [H]

Synonym: t2747

Alternate gene names: 29143106

Gene position: 2830922-2830452 (Counterclockwise)

Preceding gene: 29143107

Following gene: 29143105

Centisome position: 59.08

GC content: 56.26

Gene sequence:

>471_bases
GTGACTGACGTTTTACTCTGTGTCGGCAACAGCATGATGGGCGACGACGGCGCAGGCCCATTACTGGCGGAAATGTGCGC
GGCGCAGCCGAAGGGCAACTGGGTTGTCATCGATGGCGGCAGCGCGCCGGAAAACGACATTGTGGCGATTCGCGAACTGC
GCCCGCAGCGTCTGTTGATTGTCGACGCCACCGATATGGGACTTAATCCGGGCGAGATCCGCATCATCGATCCGGATGAT
ATCGCCGAAATGTTTATGATGACCACCCACAATATGCCGCTGAACTACCTTATCGATCTGCTGAAAGAGGATGTCGGCGA
GGTGATTTTTGTTGGTATCCAGCCGGATATCGTCGGGTTTTATTATCCGATGACTCAACCGATTAAGGACGCGGTAAACA
TCGTCTATCAGCGCCTGGAAGGCTGGCAGGGAAACGGCGGATTTGCCGCGCTGGACGCGCCGGAGGCGTAA

Upstream 100 bases:

>100_bases
GCGCAGACCCCGCAGCAGCAAGAATGGAGCAAACTGATGCTCAGTATGCTGCATGACATTCATCAGGAAAGCGCCATCTA
CCTGATGGTGAGGAGGCTTC

Downstream 100 bases:

>100_bases
AATGCGTACTCATTCTGGATGGATTTGTGTTACCCGGCATAGGCGGTAAGAAGAACGTGAAGATTATCGTATGGAGCGGA
CTGCTATTTCTCTGCCTCGC

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTDVLLCVGNSMMGDDGAGPLLAEMCAAQPKGNWVVIDGGSAPENDIVAIRELRPQRLLIVDATDMGLNPGEIRIIDPDD
IAEMFMMTTHNMPLNYLIDLLKEDVGEVIFVGIQPDIVGFYYPMTQPIKDAVNIVYQRLEGWQGNGGFAALDAPEA

Sequences:

>Translated_156_residues
MTDVLLCVGNSMMGDDGAGPLLAEMCAAQPKGNWVVIDGGSAPENDIVAIRELRPQRLLIVDATDMGLNPGEIRIIDPDD
IAEMFMMTTHNMPLNYLIDLLKEDVGEVIFVGIQPDIVGFYYPMTQPIKDAVNIVYQRLEGWQGNGGFAALDAPEA
>Mature_155_residues
TDVLLCVGNSMMGDDGAGPLLAEMCAAQPKGNWVVIDGGSAPENDIVAIRELRPQRLLIVDATDMGLNPGEIRIIDPDDI
AEMFMMTTHNMPLNYLIDLLKEDVGEVIFVGIQPDIVGFYYPMTQPIKDAVNIVYQRLEGWQGNGGFAALDAPEA

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=155, Percent_Identity=91.6129032258064, Blast_Score=290, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 16966; Mature: 16835

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLLCVGNSMMGDDGAGPLLAEMCAAQPKGNWVVIDGGSAPENDIVAIRELRPQRLLI
CCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEE
VDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDLLKEDVGEVIFVGIQPDIVGF
EECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEE
YYPMTQPIKDAVNIVYQRLEGWQGNGGFAALDAPEA
ECCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TDVLLCVGNSMMGDDGAGPLLAEMCAAQPKGNWVVIDGGSAPENDIVAIRELRPQRLLI
CCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEE
VDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDLLKEDVGEVIFVGIQPDIVGF
EECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEE
YYPMTQPIKDAVNIVYQRLEGWQGNGGFAALDAPEA
ECCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]