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The map label for this gene is aroF [H]

Identifier: 29142994

GI number: 29142994

Start: 2702579

End: 2703649

Strand: Reverse

Name: aroF [H]

Synonym: t2625

Alternate gene names: 29142994

Gene position: 2703649-2702579 (Counterclockwise)

Preceding gene: 29142997

Following gene: 29142993

Centisome position: 56.42

GC content: 53.13

Gene sequence:

>1071_bases
ATGCAAAAAGACGCGCTGAATAACGTACGTATCACCGATGAACAGGTATTAATGACGCCGGAGCAGCTTAAAGCGGCCTT
TCCGTTGAGCCTGGCGCAGGAAGCGCAGATAGCGCAGTCCCGGGGAATCATTTCTGACATTATTGCCGGGCGCGATCCGC
GTCTGTTGGTGGTATGCGGTCCTTGTTCTATTCACGATCCTGAAACCGCTCTGGAATATGCCCGTCGATTTAAAGCCCTT
GCCGCAGAGGTCAGCGATAGCCTCTATCTGGTAATGCGCGTCTATTTTGAAAAGCCGCGGACTACCGTCGGCTGGAAAGG
GCTGATTAACGATCCTCACATGGATGGCTCATTTGATGTGGAAGCCGGGTTGAAAATAGCGCGTCAGCTACTGGTGGAAC
TGGTGAATATGGGGTTGCCATTGGCGACCGAAGCGTTGGATCCGAACAGCCCGCAATACCTGGGCGATCTGTTTAGCTGG
TCGGCGATAGGCGCGCGCACAACCGAATCGCAAACCCACCGCGAAATGGCGTCTGGTCTTTCTATGCCGGTCGGCTTTAA
AAACGGCACGGATGGCAGCCTGGCGACAGCGATTAACGCCATGCGCGCCGCTGCGCAACCTCATCGTTTTGTTGGCATTA
ACCAGGCCGGTCAGGTTGCGTTATTGCAAACCCAGGGAAATCCGCATGGCCATGTGATTCTGCGTGGCGGCAAAGCGCCA
AACTATAGCCCGGCAGATGTCGCTCAGTGTGAAAAAGAGATGGAACAGGCGGGGCTACGTCCTTCGCTGATGGTAGATTG
CAGTCATGGTAACTCCAATAAAGATTATCGCCGCCAGCCAGCCGTTGCCGAATCTGTGGTTGCGCAGATTAAAGATGGCA
ATCGTTCAATCATTGGCTTAATGATTGAAAGTAATATTCATGAGGGTAATCAGTCTTCCGAACAGCCGCGCAGCGAAATG
AAGTATGGCGTTTCCGTCACCGATGCTTGTATTAGCTGGGAGATGACCGATGCCCTGTTACGTGAAATTCATAAAGATTT
GAGCGGCCAGCTGGCGGTGCGCGTCGCATAA

Upstream 100 bases:

>100_bases
TTTACGTAATATGGATTGAAATCTTTACTTTTTGTGGTATGGTTTAAACATCCTCGTGGAGGATCAACTATCGCAAACAA
GAAAAGACAGGATCGCCATC

Downstream 100 bases:

>100_bases
GAGGTTGTTATGGTTGCTGAATTGACCGCGTTACGCGATCAAATAGATGATGTCGATAAAGCGTTGTTGAATTTACTGGC
TAAGCGCCTGGAACTGGTTG

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQKDALNNVRITDEQVLMTPEQLKAAFPLSLAQEAQIAQSRGIISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLKIARQLLVELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPHGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHKDLSGQLAVRVA

Sequences:

>Translated_356_residues
MQKDALNNVRITDEQVLMTPEQLKAAFPLSLAQEAQIAQSRGIISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLKIARQLLVELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPHGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHKDLSGQLAVRVA
>Mature_356_residues
MQKDALNNVRITDEQVLMTPEQLKAAFPLSLAQEAQIAQSRGIISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLKIARQLLVELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPHGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHKDLSGQLAVRVA

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=356, Percent_Identity=96.3483146067416, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1786969, Length=337, Percent_Identity=54.3026706231454, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1787996, Length=344, Percent_Identity=47.6744186046512, Blast_Score=333, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6320240, Length=342, Percent_Identity=50.5847953216374, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319726, Length=345, Percent_Identity=51.304347826087, Blast_Score=354, Evalue=1e-98,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 38714; Mature: 38714

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDALNNVRITDEQVLMTPEQLKAAFPLSLAQEAQIAQSRGIISDIIAGRDPRLLVVCG
CCCCCCCCCEECCCCEEECHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEC
PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
EAGLKIARQLLVELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPHGHVILRGGKAP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEEECCCCC
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEE
MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHKDLSGQLAVRVA
EEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MQKDALNNVRITDEQVLMTPEQLKAAFPLSLAQEAQIAQSRGIISDIIAGRDPRLLVVCG
CCCCCCCCCEECCCCEEECHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEC
PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
EAGLKIARQLLVELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPHGHVILRGGKAP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEEECCCCC
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEE
MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHKDLSGQLAVRVA
EEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]