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The map label for this gene is yafJ [H]

Identifier: 29142921

GI number: 29142921

Start: 2614977

End: 2615744

Strand: Reverse

Name: yafJ [H]

Synonym: t2539

Alternate gene names: 29142921

Gene position: 2615744-2614977 (Counterclockwise)

Preceding gene: 29142922

Following gene: 29142919

Centisome position: 54.59

GC content: 51.17

Gene sequence:

>768_bases
ATGTGCGAACTGCTTGGGATGAGCGCCAATGTGCCGATGGATATCTGCTTTAGCTTCACCGGACTGGTGCAGCGTGGCGG
CGGAACCGGGCCGCATAAAGACGGCTGGGGAATTACCTTTTACGAAGGAAAGGGCTGTCGCACGTTTAAAGATCCGCAAC
CGAGCTACCATTCGCCGATTGCGAAACTGGTACAGAATTACCCGATCAAGTCCTGCTCGGTTATCGCTCATATCCGTCAG
GCTAATCGCGGTGAAGTAGCGCTGGAAAATACACACCCTTTTACCCGGGAATTATGGGGACGCAACTGGACTTATGCTCA
TAACGGTCAGCTCAACGGTTATAAGTCGCTGGAAACCGGTAATTTTCGTCCGGTGGGGGAAACCGACAGCGAAAAAGCGT
TTTGCTGGCTATTGCATAAATTAACGCAGCGTTATCCCCGCACGCCGGGCAACATGACAGCGGTATTTAAATATATTGCG
ACACTGGCGACGGTATTACGTGAAAAAGGCGTTTTTAACATGCTGCTGTCAGATGGGCGATATGTGATGGCGTTTTGCTC
AACCCATTTGCACTGGATCACTCGCCGGGCGCCGTTTGGCGTAGCCACGCTGGTGGATCAGGATATGGAAATTGATTTCA
GTTCACAGACCACGCCAAATGACGTGGTTACCGTGATTGCTACCCAGCCGCTAACGGGGAATGAAACCTGGCAAAAGATT
ATGCCAGGCGAATGGGCGTTATTTTGCCTCGGAGAGCGTATAATTTGA

Upstream 100 bases:

>100_bases
CGGGCATTGTGCCGGATGGCGACGCAAGCGTCTTATCCGGCCTACGAGCTGTGCTTTGCTGGTCTTATCAGGCAATTAAC
CGTTTTAAGGTAGGTGATGT

Downstream 100 bases:

>100_bases
GGCCAGCTGTGGCTGAACGACTTCGTGACTCAACGGTTTGCTCACGACGTAACGTCCATCAACGATAGAAACAGTTGGCG
GCTTATGCGTCTGTTCAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCELLGMSANVPMDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPIAKLVQNYPIKSCSVIAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIA
TLATVLREKGVFNMLLSDGRYVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWALFCLGERII

Sequences:

>Translated_255_residues
MCELLGMSANVPMDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPIAKLVQNYPIKSCSVIAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIA
TLATVLREKGVFNMLLSDGRYVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWALFCLGERII
>Mature_255_residues
MCELLGMSANVPMDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPIAKLVQNYPIKSCSVIAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIA
TLATVLREKGVFNMLLSDGRYVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWALFCLGERII

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=92.9411764705882, Blast_Score=509, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28700; Mature: 28700

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPMDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
AKLVQNYPIKSCSVIAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETG
HHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIATLATVLREKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
YVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHH
MPGEWALFCLGERII
CCCCEEEEECCCCCC
>Mature Secondary Structure
MCELLGMSANVPMDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
AKLVQNYPIKSCSVIAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETG
HHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIATLATVLREKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
YVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHH
MPGEWALFCLGERII
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]