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The map label for this gene is prpC [H]

Identifier: 29142881

GI number: 29142881

Start: 2569380

End: 2570549

Strand: Reverse

Name: prpC [H]

Synonym: t2495

Alternate gene names: 29142881

Gene position: 2570549-2569380 (Counterclockwise)

Preceding gene: 29142882

Following gene: 29142880

Centisome position: 53.64

GC content: 55.38

Gene sequence:

>1170_bases
ATGACAGACACGACGATCCTGCAAAACAACACGCATGTCATTAAGCCTAAAAAATCGGTCGCGCTTTCCGGCGTACCTGC
CGGAAATACCGCTCTGTGTACCGTGGGAAAAAGCGGTAACGATCTGCACTATCGCGGTTACGACATTCTCGATCTGGCGG
AGCACTGCGAATTTGAAGAAGTCGCGCATTTACTGATCCACGGAAAATTACCGACCCGCGACGAGCTGAACGCGTATAAA
AGCAAGTTAAAAGCGCTGCGCGGATTACCCGCCAATGTCCGTACCGTTCTGGAAGCGTTACCGGCGGCCTCGCACCCGAT
GGACGTGATGCGTACCGGCGTTTCCGCGCTGGGCTGCACCCTGCCGGAAAAAGAGGGACATACCGTCTCCGGCGCGCGCG
ATATTGCCGATAAGCTGTTGGCTTCGCTCAGCTCTATCCTTCTTTACTGGTATCACTACAGCCACAACGGCGAACGTATT
CAGCCGGAAACCGACGATGATTCCATCGGCGGCCATTTCCTGCATCTGCTGCACGGTGAAAAGCCAACCCAAAGCTGGGA
AAAGGCGATGCATATTTCGCTGGTGCTGTACGCCGAGCATGAGTTCAACGCCTCGACGTTTACCAGCCGGGTAATTGCCG
GGACCGGCTCGGATGTCTACTCCGCGATTATCGGCGCGATTGGCGCGCTGCGCGGCCCGAAACACGGCGGAGCGAATGAG
GTGTCGCTGGAAATTCAACAGCGTTATGAAACACCGGACGAGGCAGAGGCGGATATCCGTAAACGCGTGGAAAACAAAGA
GGTGGTGATTGGCTTTGGACATCCGGTTTACACCATCGCCGACCCGCGCCACCAGGTGATCAAACGGGTGGCGAAACAGC
TTTCAGAAGAAGGCGGCTCGCTGAAGATGTACCACATCGCCGACCGTCTGGAAACGGTGATGTGGGAGACCAAAAAGATG
TTCCCGAATCTCGACTGGTTTTCGGCGGTCTCCTACAACATGATGGGCGTCCCTACCGAAATGTTCACCCCGCTGTTTGT
CATCGCCCGCGTTACCGGCTGGGCGGCGCACATTATTGAACAGCGTCAGGACAACAAAATTATTCGTCCTTCCGCCAATT
ATACCGGCCCGGAAGATCGCGTATTTGTGCCGATCGAAAAGCGTTGCTAA

Upstream 100 bases:

>100_bases
GCCGCCATCAGGCAATGCCGGATGGCACTACGTTTATCCCGCCTACGGTACGGCCTACACGCCTCAATACCCTACACATT
ACAATAATGACGAGGACAAT

Downstream 100 bases:

>100_bases
TTAAAACCTCTAAATATAAAAACAGGAGTTGTACCCTATGTCCGCACATATTTCGAACGTTCGCCCAGACTTTGACCGTG
AAATCGTTGATATCGTTGAT

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTDTTILQNNTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEEVAHLLIHGKLPTRDELNAYK
SKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
QPETDDDSIGGHFLHLLHGEKPTQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDVYSAIIGAIGALRGPKHGGANE
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSEEGGSLKMYHIADRLETVMWETKKM
FPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYTGPEDRVFVPIEKRC

Sequences:

>Translated_389_residues
MTDTTILQNNTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEEVAHLLIHGKLPTRDELNAYK
SKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
QPETDDDSIGGHFLHLLHGEKPTQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDVYSAIIGAIGALRGPKHGGANE
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSEEGGSLKMYHIADRLETVMWETKKM
FPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYTGPEDRVFVPIEKRC
>Mature_388_residues
TDTTILQNNTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEEVAHLLIHGKLPTRDELNAYKS
KLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQ
PETDDDSIGGHFLHLLHGEKPTQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDVYSAIIGAIGALRGPKHGGANEV
SLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSEEGGSLKMYHIADRLETVMWETKKMF
PNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYTGPEDRVFVPIEKRC

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=388, Percent_Identity=96.1340206185567, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI1786939, Length=369, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=357, Percent_Identity=25.2100840336134, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=389, Percent_Identity=23.9074550128535, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=331, Percent_Identity=25.9818731117825, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640124, Length=330, Percent_Identity=25.4545454545455, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640126, Length=330, Percent_Identity=25.4545454545455, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 43213; Mature: 43082

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTILQNNTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEE
CCCCEEEECCCEEECCCCCEEECCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHCCHHH
VAHLLIHGKLPTRDELNAYKSKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCT
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
LPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQPETDDDSIGGHFLHLLHGE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHEEEECCCC
KPTQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDVYSAIIGAIGALRGPKHGGANE
CCHHHHHHHEEEEEEEEEECCCCCHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCCC
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSEEGGS
EEEEHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCC
LKMYHIADRLETVMWETKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIE
EEEEEHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QRQDNKIIRPSANYTGPEDRVFVPIEKRC
HHCCCCEECCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
TDTTILQNNTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEE
CCCEEEECCCEEECCCCCEEECCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHCCHHH
VAHLLIHGKLPTRDELNAYKSKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCT
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
LPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQPETDDDSIGGHFLHLLHGE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHEEEECCCC
KPTQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDVYSAIIGAIGALRGPKHGGANE
CCHHHHHHHEEEEEEEEEECCCCCHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCCC
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSEEGGS
EEEEHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCC
LKMYHIADRLETVMWETKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIE
EEEEEHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QRQDNKIIRPSANYTGPEDRVFVPIEKRC
HHCCCCEECCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]