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The map label for this gene is yajF [H]

Identifier: 29142858

GI number: 29142858

Start: 2544731

End: 2545639

Strand: Reverse

Name: yajF [H]

Synonym: t2471

Alternate gene names: 29142858

Gene position: 2545639-2544731 (Counterclockwise)

Preceding gene: 29142860

Following gene: 29142855

Centisome position: 53.12

GC content: 57.76

Gene sequence:

>909_bases
GTGCGTATTGGTATCGATTTAGGCGGCACAAAAACGGAAGTCATTGCGCTTGATGATGCAGGAGAGCAGCGGTTTCGCCA
TCGTCTGCCTACGCCGCGGGAGGATTATCAGCAAACGATAGAAACCATCGCTACGCTGGTCGACATGGCGGAACAGGCGA
CCGGCCAGACCGGTAGCGTTGGCATTGGCATTCCCGGTTCTCTCTCGCCTTACACCGGCGTGGTAAAAAACGCTAACTCT
ACGTGGCTTAACGGCCAGCCGTTCGATAGCGATGTGAGCCGTCGTCTGAAGCGTGAAGTGCGGTTAGCCAACGATGCCAA
TTGCCTGGCGGTATCAGAGGCGGTAGATGGCGCGGCGGCGGGGGCGCAAACGGTATTTGCGGTGATTATCGGTACCGGGT
GCGGCGCGGGCGTAGCGCTAAATGGCCGGGCGCACATCGGCGGCAACGGTACGGCAGGCGAATGGGGCCACAATCCGTTG
CCGTGGATGGATGACGATGAGTTGCGTTACCGCGAAGAAATTCCCTGTTATTGCGGTAAACAAGGCTGTATCGAAACCTT
TATTTCCGGTACGGGATTCGCCACGGATTACCAGCGTTTGAGCGGTAAAACGCTAAAAGGCGATGAGATTATTCGTCTGG
TCGACGCGCAGGACGCCGTGGCGGAACTGGCGATAAGCCGTTATGAGCTACGGCTGGCGAAGGCATTATCTCATGTCGTG
AATATTCTGGATCCGGATGTCATTGTGCTGGGCGGCGGTATGAGCAATGTTGAGCGCCTGTACAAAACGGTTCCGTCGCT
GATGAAATCGTTTGTTTTTGGCGGCGAGTGCGAGACGCCGGTACGTAAGGCGCGGCATGGCGATTCGAGCGGCGTGCGCG
GCGCGGCCTGGCTGTGGCCGCTGGCGTAA

Upstream 100 bases:

>100_bases
AATCCAGTCGGTATAGTAACGAATTGTCGGCAGGCTTGCATTGCCAATCAGAACGGTGAATTCTACCCTGTTGATATTCC
CAAAAAATGAGGAGCGGTAT

Downstream 100 bases:

>100_bases
CGTTTCTAGCCGGATGGCGTCATCCGGCAACCGGCGTGACCGATGGCTGCTTGTGTTTTAGACGTCCGTATACCAGCAAC
GACGACATGGCGGAAAACGA

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSVGIGIPGSLSPYTGVVKNANS
TWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
PWMDDDELRYREEIPCYCGKQGCIETFISGTGFATDYQRLSGKTLKGDEIIRLVDAQDAVAELAISRYELRLAKALSHVV
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWPLA

Sequences:

>Translated_302_residues
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSVGIGIPGSLSPYTGVVKNANS
TWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
PWMDDDELRYREEIPCYCGKQGCIETFISGTGFATDYQRLSGKTLKGDEIIRLVDAQDAVAELAISRYELRLAKALSHVV
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWPLA
>Mature_302_residues
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSVGIGIPGSLSPYTGVVKNANS
TWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
PWMDDDELRYREEIPCYCGKQGCIETFISGTGFATDYQRLSGKTLKGDEIIRLVDAQDAVAELAISRYELRLAKALSHVV
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWPLA

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=88, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1787363, Length=301, Percent_Identity=38.8704318936877, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1786891, Length=274, Percent_Identity=24.0875912408759, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790522, Length=206, Percent_Identity=29.126213592233, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787878, Length=216, Percent_Identity=26.3888888888889, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32290; Mature: 32290

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
GIGIPGSLSPYTGVVKNANSTWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAA
EECCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDDDELRYREEIPCYCGK
HHHHEEHHEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYQRLSGKTLKGDEIIRLVDAQDAVAELAISRYELRLAKALSHVV
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWP
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEECC
LA
CC
>Mature Secondary Structure
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
GIGIPGSLSPYTGVVKNANSTWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAA
EECCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDDDELRYREEIPCYCGK
HHHHEEHHEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYQRLSGKTLKGDEIIRLVDAQDAVAELAISRYELRLAKALSHVV
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWP
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEECC
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]