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The map label for this gene is ramA [H]

Identifier: 29142686

GI number: 29142686

Start: 2357626

End: 2357967

Strand: Reverse

Name: ramA [H]

Synonym: t2287

Alternate gene names: 29142686

Gene position: 2357967-2357626 (Counterclockwise)

Preceding gene: 29142690

Following gene: 29142681

Centisome position: 49.21

GC content: 50.29

Gene sequence:

>342_bases
ATGACCATTTCCGCTCAGGTTATCGACACGATTGTCGAGTGGATTGATGATAATTTGAATCAGCCGTTACGTATTGATGA
TATTGCCCGTCATGCGGGGTATTCCAAGTGGCACCTGCAGCGCCTTTTTATGCAGTACAAAGGGGAGAGTCTGAGGCGCT
ACGTGCGTGAACGGAAGCTAAAACTGGCGGCGCGCGACCTGCGCGACACCGACCAGAAGGTGTATGATATTTGTCTCAAG
TATGGTTTTGATTCGCAGCAAACCTTTACGCGCATTTTTACCCGCACGTTCAACCTGCCGCCAGGCGCTTATCGTAAAGA
AAAGCATGGCCGTACGCATTGA

Upstream 100 bases:

>100_bases
AGCCGTCTCACAATTTGTGTGGTTATTACTATGCTTATTGCTGTTGCCGTAAATGTGCGGTGCGGGAGCCGCTGACGAGT
TTGATAGAGGGGAGAGCACG

Downstream 100 bases:

>100_bases
AATGTTTAATCGGCCAGCGAAAACCAGCGCCGCCAGATAAAGCGAATAACATAATATTCCAGCGTGCCGAGCAACAGAAA
CCATAAACAAAACAACAAAG

Product: transcriptional activator RamA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 112

Protein sequence:

>113_residues
MTISAQVIDTIVEWIDDNLNQPLRIDDIARHAGYSKWHLQRLFMQYKGESLRRYVRERKLKLAARDLRDTDQKVYDICLK
YGFDSQQTFTRIFTRTFNLPPGAYRKEKHGRTH

Sequences:

>Translated_113_residues
MTISAQVIDTIVEWIDDNLNQPLRIDDIARHAGYSKWHLQRLFMQYKGESLRRYVRERKLKLAARDLRDTDQKVYDICLK
YGFDSQQTFTRIFTRTFNLPPGAYRKEKHGRTH
>Mature_112_residues
TISAQVIDTIVEWIDDNLNQPLRIDDIARHAGYSKWHLQRLFMQYKGESLRRYVRERKLKLAARDLRDTDQKVYDICLKY
GFDSQQTFTRIFTRTFNLPPGAYRKEKHGRTH

Specific function: Probable transcriptional activator [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=43.5643564356436, Blast_Score=106, Evalue=3e-25,
Organism=Escherichia coli, GI1790497, Length=100, Percent_Identity=45, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1790857, Length=105, Percent_Identity=44.7619047619048, Blast_Score=101, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 13576; Mature: 13444

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISAQVIDTIVEWIDDNLNQPLRIDDIARHAGYSKWHLQRLFMQYKGESLRRYVRERKL
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
KLAARDLRDTDQKVYDICLKYGFDSQQTFTRIFTRTFNLPPGAYRKEKHGRTH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TISAQVIDTIVEWIDDNLNQPLRIDDIARHAGYSKWHLQRLFMQYKGESLRRYVRERKL
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
KLAARDLRDTDQKVYDICLKYGFDSQQTFTRIFTRTFNLPPGAYRKEKHGRTH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2193928; 7551053 [H]