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The map label for this gene is entE [H]

Identifier: 29142672

GI number: 29142672

Start: 2337933

End: 2339546

Strand: Reverse

Name: entE [H]

Synonym: t2272

Alternate gene names: 29142672

Gene position: 2339546-2337933 (Counterclockwise)

Preceding gene: 161486793

Following gene: 29142671

Centisome position: 48.82

GC content: 57.87

Gene sequence:

>1614_bases
ATGATGCGTATACCTTTCACCCGTTGGCCGGATGAATTTGCCCGCCGTTATCGTGAAAAAGGCTACTGGCAAGACGTGCC
GTTGACCGATATTCTGACCCGCCACGCTGACAGCGATAAGACGGCGGTCATTGAAGGCGAGCGCGCATTCAGCTATCGCC
AGCTAAACCAGGCCGCGGATAATCTGGCCTGCAGTTTACGCCGTCAGGGCATCAAACCTGGCGAAACCGCTCTGGTACAA
CTGGGGAATGTACCGGAACTGTATATCACCTTTTTCGCCCTGTTGAAGCTTGGCGTAGCGCCCGTTCTGGCGCTGTTTAG
CCATCAACGTACCGAACTTAACGCCTATGCGATGCAGATCGCGCCGACGCTGGTGATTGCCGATCGCCAACATACGCTGT
TCGCCGGGGAGGACTTTCTCAACAGGTTTGTGGCTGAACACCGCTCTGCGCGGGTGGTGTTATTGCGCAACGACGACGGC
GATCACAGCCTGGACGCGGCGATGCGGCAGGCGGCGGAAGGTTTCACCGCCACGCCATCACCTGCTGACGAAGTGGCCTA
CTTTCAGCTTTCCGGCGGTACTACCGGCACGCCAAAGCTTATTCCCCGTACCCATAACGACTATTACTACAGCGTGCGCC
GCAGCAATGAGATTTGCGGTTTCAACGAGGAGACGCGTTTTCTGTGCGCGATTCCCGCCGCGCATAACTACGCCATGAGT
TCGCCGGGCGCGCTGGGCGTTTTTCTCGCCAAAGGAACGGTAGTGCTGGCGACCGATCCTGGCGCCACGCTCTGTTTCCC
GCTGATCGAAAAACACCAGATTAATGCCACGGCGCTGGTGCCGCCCGCGGTCAGTCTATGGCTACAGGCTATCCAGGAGT
GGGGCGGCAATGCGCCGCTGGCGTCATTAAGGTTATTGCAGGTTGGCGGCGCGCGGCTTTCTGCGACGCTGGCCGCCCGT
ATTCCGGCTGAAATTGGCTGTCAGTTGCAGCAGGTCTTCGGCATGGCGGAAGGGTTAGTGAACTATACCCGGCTGGACGA
TAGCCCGGAACGGATTATCAATACCCAGGGAAGACCCATGTGTCCGGACGACGAAGTGTGGGTGGCGGATGCCGACGGGA
ATCCACTGCCGCCGGGCGAGATTGGTCGTCTGATGACGCGCGGCCCCTATACTTTTCGCGGCTATTTCAACAGTCCGCAA
CACAATGTCAGCGCCTTTGACGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCCATTGATCAAGACGGCTACATCAC
CGTTCACGGGCGTGAAAAAGATCAGATCAACCGGGGCGGCGAGAAGATAGCCGCCGAAGAGATAGAAAACCTGTTACTGC
GCCACCCGGCGGTGATCCATGCGGCGCTGGTCAGCATGGAAGATGAACTGCTGGGGGAAAAAAGTTGCGCATATCTGGTG
GTAAAAGAGCCGCTGCGAGCGGTACAGGTACGCCGTTTCCTGCGAGAGCAGGGCGTGGCGGAATTTAAATTACCGGATCG
CGTGGAGTGCGTTGCGTCACTGCCGCTGACGCCGGTTGGTAAAGTCGATAAAAAACAATTACGCCAGCGGTTGGCGTCAC
GTTCACCGCTCTGA

Upstream 100 bases:

>100_bases
CGCCGGGATTGTTCCTGCCTCTTCGCCAGTCGGCGAGTGGCGGGAAACCGGCGTCAAGCTCTCTACGATGTTGAACGTTT
TTGGACTGCATTAAGGAACG

Downstream 100 bases:

>100_bases
AGGAGAAAGAGAGATGGCAATCCCGAAACTACAGTCTTACGCGCTGCCCACCGCACTGGATATCCCGACCAACAAAGTGA
ACTGGGCATTTGAGCCGGAG

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MMRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQ
LGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNRFVAEHRSARVVLLRNDDG
DHSLDAAMRQAAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMS
SPGALGVFLAKGTVVLATDPGATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQ
HNVSAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLV
VKEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

Sequences:

>Translated_537_residues
MMRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQ
LGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNRFVAEHRSARVVLLRNDDG
DHSLDAAMRQAAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMS
SPGALGVFLAKGTVVLATDPGATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQ
HNVSAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLV
VKEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
>Mature_537_residues
MMRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQ
LGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNRFVAEHRSARVVLLRNDDG
DHSLDAAMRQAAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMS
SPGALGVFLAKGTVVLATDPGATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQ
HNVSAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLV
VKEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=24.7524752475248, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI122937307, Length=511, Percent_Identity=24.26614481409, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=23.5887096774194, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI58082049, Length=561, Percent_Identity=23.3511586452763, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI157311624, Length=563, Percent_Identity=22.7353463587922, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311622, Length=563, Percent_Identity=22.7353463587922, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI156151445, Length=192, Percent_Identity=31.25, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=22.8070175438596, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=23.4732824427481, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI187761345, Length=445, Percent_Identity=23.5955056179775, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI187761343, Length=445, Percent_Identity=23.5955056179775, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI8923896, Length=572, Percent_Identity=20.8041958041958, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI253970489, Length=413, Percent_Identity=22.2760290556901, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=85.9550561797753, Blast_Score=953, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=31.9702602230483, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=26.0245901639344, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788107, Length=570, Percent_Identity=22.9824561403509, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786529, Length=535, Percent_Identity=23.5514018691589, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790505, Length=545, Percent_Identity=23.4862385321101, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789201, Length=367, Percent_Identity=23.433242506812, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786801, Length=526, Percent_Identity=22.8136882129278, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=544, Percent_Identity=26.2867647058824, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=26.6666666666667, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=25, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=25, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=25, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=392, Percent_Identity=26.7857142857143, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=528, Percent_Identity=24.4318181818182, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=363, Percent_Identity=24.5179063360882, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.4126984126984, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=23.2741617357002, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=351, Percent_Identity=27.0655270655271, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=532, Percent_Identity=23.3082706766917, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=538, Percent_Identity=22.3048327137546, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=538, Percent_Identity=22.3048327137546, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=506, Percent_Identity=23.5177865612648, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=26.5193370165746, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=563, Percent_Identity=22.3801065719361, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=24.812030075188, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=22.920892494929, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859661, Length=375, Percent_Identity=24.5333333333333, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=524, Percent_Identity=22.9007633587786, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24656500, Length=501, Percent_Identity=21.7564870259481, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=436, Percent_Identity=24.0825688073394, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667955, Length=540, Percent_Identity=21.1111111111111, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=21.1111111111111, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=494, Percent_Identity=21.8623481781377, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358303, Length=342, Percent_Identity=23.0994152046784, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=328, Percent_Identity=25.609756097561, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=510, Percent_Identity=23.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648253, Length=475, Percent_Identity=22.1052631578947, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648255, Length=469, Percent_Identity=22.1748400852878, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24667959, Length=402, Percent_Identity=21.3930348258706, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 59355; Mature: 59355

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAAD
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
NLACSLRRQGIKPGETALVQLGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQI
HHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEE
APTLVIADRQHTLFAGEDFLNRFVAEHRSARVVLLRNDDGDHSLDAAMRQAAEGFTATPS
CCEEEEECCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
PADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMS
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCC
SPGALGVFLAKGTVVLATDPGATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPL
CCCCEEEEEECCEEEEEECCCCEEHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCH
ASLRLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPM
HHEEEEHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
CPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQHNVSAFDANGFYCSGDLISI
CCCCCEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCEEECCCEEEE
DQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLV
CCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEE
VKEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
EHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MMRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAAD
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
NLACSLRRQGIKPGETALVQLGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQI
HHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEE
APTLVIADRQHTLFAGEDFLNRFVAEHRSARVVLLRNDDGDHSLDAAMRQAAEGFTATPS
CCEEEEECCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
PADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMS
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCC
SPGALGVFLAKGTVVLATDPGATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPL
CCCCEEEEEECCEEEEEECCCCEEHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCH
ASLRLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPM
HHEEEEHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
CPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQHNVSAFDANGFYCSGDLISI
CCCCCEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCEEECCCEEEE
DQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLV
CCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEE
VKEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
EHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]