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The map label for this gene is ubiF [H]

Identifier: 29142604

GI number: 29142604

Start: 2267097

End: 2268272

Strand: Reverse

Name: ubiF [H]

Synonym: t2202

Alternate gene names: 29142604

Gene position: 2268272-2267097 (Counterclockwise)

Preceding gene: 29142615

Following gene: 29142598

Centisome position: 47.33

GC content: 60.2

Gene sequence:

>1176_bases
ATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGGGGAATGGTCGGCGGCGCGCTGGCGCTGGGTCTGGCGCAGCA
AGGGTTTACGGTGATGGTAATAGAACATGCCGCGCCTGCGCCGTTTGTGGCTGACAGCCAGCCTGACGTGCGGATTTCGG
CCATTAGCGCGGCATCCGTGGCGCTGCTCAAGGGGCTGGGCGTTTGGGAGGCGGTGCAGGGGATGCGTAGCCATCCTTAT
CGGCGACTGGAGACCTGGGAGTGGGAAAACGCCCATGTGGTGTTCGACGCCGCTGAACTCAAGCTGCCGCTGTTAGGCTA
TATGGTAGAGAACAACGTCCTGCAACAGGCGCTGTGGCAGGCGCTGGAAGCGCACCCCGGCGTGACGCTGCGCGTTCCGG
CTTCACTGGCGGCATTGCACCGCCGCCACGATGGTTATGCGCTGGAACTGGCCGACGGCGAATGGGTAACGCCGAAGTTA
GTGATTGGCGCCGACGGCGCGAATTCGCAGGTTCGGCAAATGGCGGGAATTGGCATTCATGCCTGGCAATATGCGCAGTC
GTGTATGCTTATCACCGTCAAGTGTGAAAATGCGCCAGGCGACAGCACCTGGCAGCAGTTTACGCCAACGGGGCCGCGCG
CCTTTTTGCCGCTGTTCGACGATTGGGCGTCGCTGGTGTGGTATGACGCTCCGGCACGTATTCGCCAGCTACAGAGTTTA
TCGATGACGCAATTGCAGGTGGAAATTAACCAGCACTTTCCGGCGCGTCTGGGGGCGGTGATGCCTGTCGCGGCGGGCGC
GTTTCCGCTAACCCGTCGTCACGCGTTGCAGTATGCGCAACCGGGGCTGGCGCTGGTAGGCGACGCGGCGCATACCATTC
ATCCGCTGGCCGGGCAGGGCGTCAACCTGGGGTATCGGGATGTTGATGCGTTGATTGATGTGCTGGCGAGTGCCCGTAGC
TATGGCGAGTCCTGGGCCAGCCATTCGGTGTTGAAACGCTATCAGACGCGGCGCATGGCAGATAATTTTATGATGCAAAG
TGGAATGGATCTGTTTTATGCCGGGTTCAGCAATGAACTGCCGCCACTGCGTATTTTACGCAATATGGGGCTAATGGCGG
CGCAGCGCGCAGGCGTGCTAAAACGTCAGGCGCTGAAGTATGCGTTGGGGTTGTAG

Upstream 100 bases:

>100_bases
GTGTAATGCTTTGGCGCGGTTGTGACCAGTATGACTGACGTCAGCCCTAATGGGTAAAAAATCCTGTAAACTTGTCTAAA
ACGTAACAGGATGAATGACC

Downstream 100 bases:

>100_bases
TTTGTGTTATGCCGGGTGGCGGCTATACCTTACCCGGCCTGTATTCGCCCGTAGGCCTGATAAGCGTAGCGCCATCAGGC
TGTCCGAATTGCCAGAAAAG

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVADSQPDVRISAISAASVALLKGLGVWEAVQGMRSHPY
RRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQALEAHPGVTLRVPASLAALHRRHDGYALELADGEWVTPKL
VIGADGANSQVRQMAGIGIHAWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYAQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLASARS
YGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNELPPLRILRNMGLMAAQRAGVLKRQALKYALGL

Sequences:

>Translated_391_residues
MTNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVADSQPDVRISAISAASVALLKGLGVWEAVQGMRSHPY
RRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQALEAHPGVTLRVPASLAALHRRHDGYALELADGEWVTPKL
VIGADGANSQVRQMAGIGIHAWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYAQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLASARS
YGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNELPPLRILRNMGLMAAQRAGVLKRQALKYALGL
>Mature_390_residues
TNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVADSQPDVRISAISAASVALLKGLGVWEAVQGMRSHPYR
RLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQALEAHPGVTLRVPASLAALHRRHDGYALELADGEWVTPKLV
IGADGANSQVRQMAGIGIHAWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSLS
MTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYAQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLASARSY
GESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNELPPLRILRNMGLMAAQRAGVLKRQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=33.6787564766839, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI32967307, Length=347, Percent_Identity=33.4293948126801, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=85.9335038363171, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=39.7959183673469, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1789274, Length=372, Percent_Identity=32.7956989247312, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=27.8422273781903, Blast_Score=168, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6321694, Length=288, Percent_Identity=28.4722222222222, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920760, Length=419, Percent_Identity=28.4009546539379, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42606; Mature: 42474

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVADSQPDVRISAISAASV
CCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEHHHHHH
ALLKGLGVWEAVQGMRSHPYRRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQ
HHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
ALEAHPGVTLRVPASLAALHRRHDGYALELADGEWVTPKLVIGADGANSQVRQMAGIGIH
HHHCCCCEEEECCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
HHHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCEECCHHHHHHHHEEECCHHHHHHHHHC
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYAQPGLALVGDAAHTIHPLAGQG
CCEEEEEEECCCCCHHHCCHHHHHCCCCCHHHHHHHHHCCCCCEEECCCHHHHCCCCCCC
VNLGYRDVDALIDVLASARSYGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRILRNMGLMAAQRAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVADSQPDVRISAISAASV
CCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEHHHHHH
ALLKGLGVWEAVQGMRSHPYRRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQ
HHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
ALEAHPGVTLRVPASLAALHRRHDGYALELADGEWVTPKLVIGADGANSQVRQMAGIGIH
HHHCCCCEEEECCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
HHHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCEECCHHHHHHHHEEECCHHHHHHHHHC
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYAQPGLALVGDAAHTIHPLAGQG
CCEEEEEEECCCCCHHHCCHHHHHCCCCCHHHHHHHHHCCCCCEEECCCHHHHCCCCCCC
VNLGYRDVDALIDVLASARSYGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRILRNMGLMAAQRAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]