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The map label for this gene is ybfF [H]

Identifier: 29142587

GI number: 29142587

Start: 2246079

End: 2246849

Strand: Direct

Name: ybfF [H]

Synonym: t2179

Alternate gene names: 29142587

Gene position: 2246079-2246849 (Clockwise)

Preceding gene: 29142584

Following gene: 29142588

Centisome position: 46.87

GC content: 56.42

Gene sequence:

>771_bases
ATGAAATTAAACATCCGAGCGCAATCTGCACAAAACCTGCACAATAATTCCCCCATCGTTCTCGTTCATGGCCTGTTTGG
CAGCCTGGACAACCTCGGCGTACTGGCGCGCGATCTGGTGACCGATCACGACATTATTCAGGTCGATATGCGCAATCATG
GTTTATCGCCGCGCGATCCGGTCATGGATTATCCGGCAATGGCCCAGGATCTTCTGGATACGCTTGACGCGCAGCAGATT
GAAAAAGCGACGTTTATTGGTCATTCCATGGGTGGAAAAGCGGTAATGGCGCTGACGGCGCTGGCGCCCGATCGCATCGA
TCGTCTGGTCGCGATTGATATCGCCCCCGTGGATTATCACGTTCGCCGCCACGATCGCATTTTCGCGGCCATTAACGCCG
TCAGCGAATCCGACGCGACATCCCGCCAACAGGCGGCAGGGATTATGCGCCAGCACCTCAACAAAGAAGGCGTGATCCAG
TTTCTGTTAAAATCCTGGGCTGAAGGCGAATGGCGTTTTAATGTTCCGGTATTATGGGAGCAGTATCCGCATATCGTCGG
CTGGGAAACCATTCCGCCCTGGGAACATCCGGCGCTGTTTATTCCAGGCGGTAACTCGCCGTACGTGACCGAAGCGTACC
GCGACGCGCTGCTGGCGCAATTCCCTTTGGCGCGCGCGCATGTGATCGCCGGCGCGGGCCACTGGGTACATGCCGAAAAA
CCAGAGGCGGTTCTGCGCGCCATCCGCCGTTATCTGCACGATAAACGCTAA

Upstream 100 bases:

>100_bases
CTGACAAAAAAACTCGGAGTAAACCTTAGAGGTTGCAGCCCAGTGGCAGAATGGTCAACATAATACCCTAACCCGACAGA
GCGAAAAAAAGAACTTTGCC

Downstream 100 bases:

>100_bases
CTGATTAAAAACTCATCGCCTGTGCGCGATTACGCGCGCGAGCGTTGGCGTCGCTTCCTCGCTGATGTATGATGGCGCGC
TATCCATCCGGGCATAAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDPVMDYPAMAQDLLDTLDAQQI
EKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYHVRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNKEGVIQ
FLLKSWAEGEWRFNVPVLWEQYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
PEAVLRAIRRYLHDKR

Sequences:

>Translated_256_residues
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDPVMDYPAMAQDLLDTLDAQQI
EKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYHVRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNKEGVIQ
FLLKSWAEGEWRFNVPVLWEQYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
PEAVLRAIRRYLHDKR
>Mature_256_residues
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDPVMDYPAMAQDLLDTLDAQQI
EKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYHVRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNKEGVIQ
FLLKSWAEGEWRFNVPVLWEQYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
PEAVLRAIRRYLHDKR

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=246, Percent_Identity=34.5528455284553, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI23200008, Length=246, Percent_Identity=34.5528455284553, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI223941839, Length=237, Percent_Identity=30.379746835443, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786902, Length=254, Percent_Identity=89.3700787401575, Blast_Score=470, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17508535, Length=264, Percent_Identity=32.9545454545455, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17507063, Length=252, Percent_Identity=26.984126984127, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321468, Length=259, Percent_Identity=30.1158301158301, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24640348, Length=270, Percent_Identity=28.1481481481481, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646055, Length=274, Percent_Identity=26.6423357664234, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28706; Mature: 28706

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDP
CEEEEECHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
VMDYPAMAQDLLDTLDAQQIEKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCEEEEEEEECCCHH
VRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNKEGVIQFLLKSWAEGEWRFNVPVLWE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCHHH
QYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
CCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHEEECCCCEEECCC
PEAVLRAIRRYLHDKR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLNIRAQSAQNLHNNSPIVLVHGLFGSLDNLGVLARDLVTDHDIIQVDMRNHGLSPRDP
CEEEEECHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
VMDYPAMAQDLLDTLDAQQIEKATFIGHSMGGKAVMALTALAPDRIDRLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCEEEEEEEECCCHH
VRRHDRIFAAINAVSESDATSRQQAAGIMRQHLNKEGVIQFLLKSWAEGEWRFNVPVLWE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCHHH
QYPHIVGWETIPPWEHPALFIPGGNSPYVTEAYRDALLAQFPLARAHVIAGAGHWVHAEK
CCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHEEECCCCEEECCC
PEAVLRAIRRYLHDKR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]