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The map label for this gene is sucB [H]

Identifier: 29142551

GI number: 29142551

Start: 2200538

End: 2201746

Strand: Reverse

Name: sucB [H]

Synonym: t2139

Alternate gene names: 29142551

Gene position: 2201746-2200538 (Counterclockwise)

Preceding gene: 29142552

Following gene: 29142550

Centisome position: 45.95

GC content: 56.41

Gene sequence:

>1209_bases
ATGAGTAGCGTAGATATTCTTGTTCCCGACCTGCCTGAGTCCGTAGCGGACGCGACCGTCGCCACCTGGCATAAAAAACC
GGGCGATGCGGTTGTTCGTGACGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCGG
ACGGCATTCTGGATGCGGTTCTGGAAGAAGAGGGGACGACTGTCACCTCTCGCCAGATCCTGGGTCGCCTGCGTGAAGGC
AACAGCGCGGGTAAAGAGACCAGCGCGAAATCTGAAGAGAAAGCGTCTACGCCGGCACAGCGCCAGCAGGCGTCGCTGGA
AGAACAAAACAACGATGCGCTCAGCCCGGCGATCCGTCGCCTGCTGGCTGAACACAATCTGGAAGCCAGCGCCATTAAAG
GCACTGGTGTGGGTGGGCGTCTGACCCGTGAAGATGTGGAAAAACATCTGGCGAAAGGCGAGTCTAAAGCACCCGCTGTT
GAACCTGCAGCACAACCCGCTCTGGGCGCGCGCGGCGAAAAACGCGTGCCAATGACCCGCCTGCGTAAGCGCGTGGCCGA
GCGTCTGCTGGAAGCGAAAAATTCCACCGCCATGCTGACGACCTTCAATGAAGTCAACATGAAGCCGATTATGGATTTGC
GTAAGCAGTATGGCGAAGTGTTTGAAAAACGTCATGGTATCCGCCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTAGTG
GAAGCGCTGAAGCGTTATCCGGAAGTGAACGCCTCTATCGACGGCGATGACGTGGTGTATCACAACTACTTTGATGTGAG
CATGGCGGTGTCTACGCCGCGCGGCCTGGTCACGCCGGTCCTGCGTGACGTGGATACGCTGGGCATGGCTGACATCGAGA
AAAAAATTAAAGAACTGGCTGTCAAAGGCCGCGACGGCAAGCTGACCGTAGAAGACCTGACCGGCGGTAACTTTACCATT
ACCAACGGCGGCGTATTCGGTTCGCTGATGTCTACCCCGATCATCAACCCGCCGCAGAGCGCGATTCTGGGAATGCATGC
TATTAAAGACAGGCCGATGGCGGTGGATGGCAAGGTGGAAATCCTGCCGATGATGTACCTGGCGCTCTCTTACGATCATC
GCCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTCACCATCAAAGAGTTGCTGGAAGATCCGACCCGTCTGCTGTTG
GACGTGTAG

Upstream 100 bases:

>100_bases
CCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTC
GATTAAATAAAGGATAAATA

Downstream 100 bases:

>100_bases
TCAGCAAGAGCATCACCTGCTACCTGTAGGCCGGATAAGGCGTTCACGCCGCCATCCGGCGATGGTGTAATGCCTGATGG
CGCGCAGCTTTCAGGCCTAC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEEEGTTVTSRQILGRLREG
NSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLEASAIKGTGVGGRLTREDVEKHLAKGESKAPAV
EPAAQPALGARGEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEVFEKRHGIRLGFMSFYVKAVV
EALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTI
TNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLL
DV

Sequences:

>Translated_402_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEEEGTTVTSRQILGRLREG
NSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLEASAIKGTGVGGRLTREDVEKHLAKGESKAPAV
EPAAQPALGARGEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEVFEKRHGIRLGFMSFYVKAVV
EALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTI
TNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLL
DV
>Mature_401_residues
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEEEGTTVTSRQILGRLREGN
SAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLEASAIKGTGVGGRLTREDVEKHLAKGESKAPAVE
PAAQPALGARGEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEVFEKRHGIRLGFMSFYVKAVVE
ALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTIT
NGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLD
V

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=57.3122529644269, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=28.476821192053, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=28.6975717439294, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=438, Percent_Identity=27.8538812785388, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=27.3148148148148, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=34.5238095238095, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=94.8148148148148, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=29.8029556650246, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=407, Percent_Identity=43.980343980344, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=31.2925170068027, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25.952380952381, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=317, Percent_Identity=31.8611987381703, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320352, Length=403, Percent_Identity=42.9280397022332, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=26.4444444444444, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24645909, Length=243, Percent_Identity=57.6131687242798, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=27.906976744186, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43858; Mature: 43727

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEEEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR
HHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLEASAIKGTGVGGRLTREDVEKHLAKGESKAPAVEPAAQPALGARGEKRVPMTR
HHHHCCCCHHEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEVFEKRHGIRLGFMSFYVKAVV
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
EALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELA
HHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
VKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGKVE
HCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCH
ILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV
HHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEEEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR
HHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLEASAIKGTGVGGRLTREDVEKHLAKGESKAPAVEPAAQPALGARGEKRVPMTR
HHHHCCCCHHEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEVFEKRHGIRLGFMSFYVKAVV
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
EALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELA
HHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
VKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGKVE
HCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCH
ILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV
HHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]