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The map label for this gene is tolA [H]

Identifier: 29142541

GI number: 29142541

Start: 2190555

End: 2191685

Strand: Reverse

Name: tolA [H]

Synonym: t2129

Alternate gene names: 29142541

Gene position: 2191685-2190555 (Counterclockwise)

Preceding gene: 29142542

Following gene: 29142540

Centisome position: 45.74

GC content: 58.09

Gene sequence:

>1131_bases
GTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAACGGGCGATAATTATTTCAGCCGTGCTGCATATCATCTTATTTGC
AGTGCTGATCTGGAGTTCGTTTGATGAGCATATAGAGGCTTCTGCCGGCGGCGGCGGCGGTTCCGCTATCGACGCGGTGA
TGGTCGATCCTGGCGCCGTTGTGCAGCAGTACAACCGTCAGCAGGATCAACAGGCCAGTGCCAGAGGCGCGGAAGAAGAG
CGTAAAAAGCTGCAACAGCAGCAAGCAGAGGAGCTGCAGCAGAAGCAGGCTGCTGAACAGGAGCGGTTGAAACAACTTGA
GAAAGAACGTTTAGCGGCTCAGGAGCAGCAAAAGCAGGCTGAAGAAGCGGCAAAACTGGCGCAACAGCAGCAACAGGCCG
AAGAAGCGGCGAAAGCGGCGGCGGACGCGAAGAAGAAAGCGGAGGCCGAGGCGGCGAAAGCGGCGGCGGACGCGAAGAAG
AAAGCAGAGGCCGAGGCGGCGAAAGCGGCGGCGGACGCGAAGAAGAAAGCAGAGGCCGAAGCGGCGAAAGCAGCGGCGGA
GGCGAAGAAGAAAGCGGAGGCCGAAGCGGCGAAGGCAGCGGCGGACGCTAAGAAGAAAGCGGATGCCGAGGCGGCGAAAG
CGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGCGGCGGCGAAAGCAGCGGCGGACGCTAAGAAGAAAGCGGCTGCCGAA
AAAGCGGCCGCCGCAGAAGGCGTCGACGATCTGCTTGGCGATCTCAGCTCGGGTAAGAATGCGCCGAAAACCGGCGGCGG
CGCGAAAGGGAATGGTCAGCCATCGAAAGATAGCGGTACATCGGGCGCTAACGGTGGGACGACAGGCGCTGATATCAGCG
CCTACGCGAAACAGATTCAGGTCGCCATTCAGAGCCGTCTGTATGATGCGAGCCTGTATCAGGGCAAACAATGTGTCTTG
CATATTAGCCTGGCGCCGGATGGCTCATTAAAAAGCATTACGTCTGAGGGCGGCGATCCGGCGCTTTGTCAGGCGGCGTT
AATGGCGACAAAAACCGCGAAAATTCCTAAACCGCCAAGCCAGGCTGTTTATGAGAAAATAAAGGATGCCAAACTAGACT
TTAAACTGTAG

Upstream 100 bases:

>100_bases
GTAAAATCGGTTGGCTTAATGACGCAGCCAATCTGAAGTCTGCGTCCCGTTGGCTTGAAAGACGGCGAGTAACAGGCGAA
CAGTTTTTTGGGAACCGAGA

Downstream 100 bases:

>100_bases
ATACTTTTCTTTATGGAAGTTGGAGCGGTTGAGTTGTTACTGGTCCTGGTGGTTTTGTGTATTTGAGTTTGTTAACATTC
TGCTAAATTATCGTGGGTCG

Product: cell envelope integrity inner membrane protein TolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQQASARGAEEE
RKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQAEEAAKAAADAKKKAEAEAAKAAADAKK
KAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAADAKKKADAEAAKAAAEAKKKADAAAAKAAADAKKKAAAE
KAAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGTTGADISAYAKQIQVAIQSRLYDASLYQGKQCVL
HISLAPDGSLKSITSEGGDPALCQAALMATKTAKIPKPPSQAVYEKIKDAKLDFKL

Sequences:

>Translated_376_residues
MSKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQQASARGAEEE
RKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQAEEAAKAAADAKKKAEAEAAKAAADAKK
KAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAADAKKKADAEAAKAAAEAKKKADAAAAKAAADAKKKAAAE
KAAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGTTGADISAYAKQIQVAIQSRLYDASLYQGKQCVL
HISLAPDGSLKSITSEGGDPALCQAALMATKTAKIPKPPSQAVYEKIKDAKLDFKL
>Mature_375_residues
SKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQQASARGAEEER
KKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQAEEAAKAAADAKKKAEAEAAKAAADAKKK
AEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAADAKKKADAEAAKAAAEAKKKADAAAAKAAADAKKKAAAEK
AAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGTTGADISAYAKQIQVAIQSRLYDASLYQGKQCVLH
ISLAPDGSLKSITSEGGDPALCQAALMATKTAKIPKPPSQAVYEKIKDAKLDFKL

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=128, Percent_Identity=80.46875, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 38805; Mature: 38673

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCHHH
VQQYNRQQDQQASARGAEEERKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAAKLAQQQQQAEEAAKAAADAKKKAEAEAAKAAADAKKKAEAEAAKAAADAKKKAEAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAAAEAKKKAEAEAAKAAADAKKKADAEAAKAAAEAKKKADAAAAKAAADAKKKAAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGTTGADISAYAKQIQ
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VAIQSRLYDASLYQGKQCVLHISLAPDGSLKSITSEGGDPALCQAALMATKTAKIPKPPS
HHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCH
QAVYEKIKDAKLDFKL
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]