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The map label for this gene is bioD

Identifier: 29142505

GI number: 29142505

Start: 2152699

End: 2153385

Strand: Reverse

Name: bioD

Synonym: t2090

Alternate gene names: 29142505

Gene position: 2153385-2152699 (Counterclockwise)

Preceding gene: 29142506

Following gene: 29142504

Centisome position: 44.94

GC content: 59.1

Gene sequence:

>687_bases
GTGACTAAACGCTATTTTGTGACCGGTACTGATACTGAAGTCGGTAAAACGGTCGCCAGTTGCGCCTTATTGCAGGCCGC
AACGCAGTTAGGTTATCAAACCGTAGGCTATAAGCCCGTCGCTTCCGGCAGCGAAATGACAACCGACGGGCTACGCAATA
GCGATGCCCTGGCCTTGCAGCGTAACAGCAGCCTGCCGCAACCTTATTCCGCTATCAATCCTTATACTTTTGCCGAGCCT
ACTTCTCCCCACATCGCTAGCGCCGACGAAGGCCGGGCGATAGACGCGGCGGTGTTATCACGCGGATTACGAACGCTGGA
GGCGCAGGCAGACTGGGTGTTGACAGAAGGGGCGGGCGGCTGGTTTACACCGCTCTCTGCCACGCTGACGTTTGCCGACT
GGGTACAGACGGAGCAGCTGCCGGTGATTCTGGTTGTTGGCGTCAAGCTGGGCTGTATTAATCACGCCATGCTGACGGCG
CTGGCGGTAGAACAGGCCGGATTACCGCTTGTCGGATGGATAGCCAACGACATGCAGCCGCCAGGCGCGCGTCATGGTGA
ATATCTCGCGACGCTTCGCCGCGTCATACCGGCCCCGCTGTTAGGGGAAATCCCGTGGCTGGGCGTTTCTCCATCGCAGG
CGGCGACAGGACAGTATCTGGACCTTAGCCCCTTGGAACGCGCCTGA

Upstream 100 bases:

>100_bases
GTATTAACCCGCGCCCGGCTTAGACAGATTCAACTGGCATGGCCGCAGCGGCAGGGGAAATATCTGCTGACGTACCACCT
TTTTATGGGAGTGATTGAAC

Downstream 100 bases:

>100_bases
CCGCGCTTAAAAACCGCCTTCTTGCAGGATCAGACGATCCTGCTGTTCTTTCGCCAGACCCTCCTGACGTCGCCATGCTA
AAAATGTGCAAAATGTGAGC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQRNSSLPQPYSAINPYTFAEP
TSPHIASADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGGWFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTA
LAVEQAGLPLVGWIANDMQPPGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA

Sequences:

>Translated_228_residues
MTKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQRNSSLPQPYSAINPYTFAEP
TSPHIASADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGGWFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTA
LAVEQAGLPLVGWIANDMQPPGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA
>Mature_227_residues
TKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQRNSSLPQPYSAINPYTFAEPT
SPHIASADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGGWFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTAL
AVEQAGLPLVGWIANDMQPPGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=75.1111111111111, Blast_Score=352, Evalue=1e-98,
Organism=Escherichia coli, GI87081944, Length=225, Percent_Identity=47.1111111111111, Blast_Score=204, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_SALTI (Q8Z890)

Other databases:

- EMBL:   AL627268
- EMBL:   AE014613
- RefSeq:   NP_455339.1
- RefSeq:   NP_805847.1
- ProteinModelPortal:   Q8Z890
- SMR:   Q8Z890
- GeneID:   1069208
- GeneID:   1247277
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t2090
- KEGG:   sty:STY0830
- HOGENOM:   HBG650065
- OMA:   PQMERIE
- ProtClustDB:   PRK00090
- BioCyc:   SENT209261:T2090-MONOMER
- BioCyc:   SENT220341:STY0830-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 24190; Mature: 24059

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
RNSSLPQPYSAINPYTFAEPTSPHIASADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGG
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
WFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTALAVEQAGLPLVGWIANDMQP
EECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCC
PGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA
CCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
TKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
RNSSLPQPYSAINPYTFAEPTSPHIASADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGG
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
WFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTALAVEQAGLPLVGWIANDMQP
EECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCC
PGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA
CCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504