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The map label for this gene is moaA [H]

Identifier: 29142501

GI number: 29142501

Start: 2144884

End: 2145873

Strand: Reverse

Name: moaA [H]

Synonym: t2085

Alternate gene names: 29142501

Gene position: 2145873-2144884 (Counterclockwise)

Preceding gene: 29142503

Following gene: 29142500

Centisome position: 44.78

GC content: 53.74

Gene sequence:

>990_bases
ATGGCTTCACAACTTACCGATGCTTTCGCGCGTAAGTTTTACTACTTGCGTTTGTCGATTACCGATGTGTGTAACTTTCG
TTGCACCTACTGTCTGCCGGATGGCTACAAACCTGGCGGCGTCACTAACAACGGTTTCCTTACCGTCGATGAAATTCGTC
GCGTTACGCGCGCTTTTGCCAGTCTTGGCACGGAAAAGGTGCGTCTTACCGGCGGCGAGCCTTCATTACGCCGCGATTTT
ACCGACATCATTGCCGCCGTGGGTGAAAATGATGCTATTCGCCAGATCGCGGTGACCACTAACGGTTATCGACTGGCGCG
CGATGCCGCGAACTGGCGCGAAGCGGGGCTGACGGGCGTCAATGTTAGCGTGGATAGTCTGGATGCGCGTCAGTTCCATG
CGATTACCGGTCAGGACAAATTCCGCCAGGTGATGGCGGGCATCGATGCCGCCTTTGACGCCGGTTTCGAAAAGGTGAAA
GTCAACACCGTGCTGATGCGCGACGTGAATCACCATCAGTTGGATACCTTCCTTGCCTGGATTCAGCCTCGCCCCATTCA
GCTACGCTTCATTGAACTTATGGAGACGGGCGAGGGGAGCGATCTCTTCCGCAAACATCATATTTCGGGTCAGGTATTAC
GTGACGAGCTGATTAAGCGCGGTTGGATCCACCAGCTCCGCCAGCGTAGCGATGGTCCGGCGCAGGTTTTTTGTCACCCC
GATTACGCCGGTGAGATCGGTCTCATTATGCCGTACGAAAAAGATTTCTGCGCCACCTGTAACCGCCTGCGTGTTTCGTC
CGTCGGTAAGCTGCATTTATGTCTGTTTGGCGACGGCGGCGTGAGTTTACGCGACCTGTTACAGGATGATGCGCAGCAAT
ACGCGCTTGAGGAACGTATTTCCGACGCGTTGCGGGAGAAAAAGCAGACCCATTTCCTTCACCAGAGCAATACTGGTATT
ACGCAAAATTTATCTTACATTGGCGGTTAA

Upstream 100 bases:

>100_bases
GGTCATTTTTGATATGGTGCTTTGGCCGTGACAACGTTTAACGTTTGCCACCAGGGCGCAGGAAGAAATGACTCCGCCTC
CCGTATTTGGAAAGGTGTAC

Downstream 100 bases:

>100_bases
TCCTTAATAAGGAGATTTCAGATGAGTCAGGTCAGCGCTGAATTTATCCCGACCCGCATCGCTATTCTTACTGTTTCCAG
CCGACGCGGCGAAGAAGACG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPGGVTNNGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVGENDAIRQIAVTTNGYRLARDAANWREAGLTGVNVSVDSLDARQFHAITGQDKFRQVMAGIDAAFDAGFEKVK
VNTVLMRDVNHHQLDTFLAWIQPRPIQLRFIELMETGEGSDLFRKHHISGQVLRDELIKRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSVGKLHLCLFGDGGVSLRDLLQDDAQQYALEERISDALREKKQTHFLHQSNTGI
TQNLSYIGG

Sequences:

>Translated_329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPGGVTNNGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVGENDAIRQIAVTTNGYRLARDAANWREAGLTGVNVSVDSLDARQFHAITGQDKFRQVMAGIDAAFDAGFEKVK
VNTVLMRDVNHHQLDTFLAWIQPRPIQLRFIELMETGEGSDLFRKHHISGQVLRDELIKRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSVGKLHLCLFGDGGVSLRDLLQDDAQQYALEERISDALREKKQTHFLHQSNTGI
TQNLSYIGG
>Mature_328_residues
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPGGVTNNGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFT
DIIAAVGENDAIRQIAVTTNGYRLARDAANWREAGLTGVNVSVDSLDARQFHAITGQDKFRQVMAGIDAAFDAGFEKVKV
NTVLMRDVNHHQLDTFLAWIQPRPIQLRFIELMETGEGSDLFRKHHISGQVLRDELIKRGWIHQLRQRSDGPAQVFCHPD
YAGEIGLIMPYEKDFCATCNRLRVSSVGKLHLCLFGDGGVSLRDLLQDDAQQYALEERISDALREKKQTHFLHQSNTGIT
QNLSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=309, Percent_Identity=38.8349514563107, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI22027536, Length=309, Percent_Identity=38.8349514563107, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=92.4012158054711, Blast_Score=637, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32566331, Length=308, Percent_Identity=35.3896103896104, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32566329, Length=308, Percent_Identity=35.3896103896104, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17561210, Length=307, Percent_Identity=34.2019543973941, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI28574490, Length=315, Percent_Identity=37.7777777777778, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28574488, Length=315, Percent_Identity=37.7777777777778, Blast_Score=208, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37002; Mature: 36871

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPGGVTNNGFLTVDEIRRVTRAFA
CCCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVGENDAIRQIAVTTNGYRLARDAANWREAGLTGV
HCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHCCHHHCCCCEE
NVSVDSLDARQFHAITGQDKFRQVMAGIDAAFDAGFEKVKVNTVLMRDVNHHQLDTFLAW
EEECCCCCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHH
IQPRPIQLRFIELMETGEGSDLFRKHHISGQVLRDELIKRGWIHQLRQRSDGPAQVFCHP
CCCCCEEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSVGKLHLCLFGDGGVSLRDLLQDDAQQYALEERI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
SDALREKKQTHFLHQSNTGITQNLSYIGG
HHHHHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPGGVTNNGFLTVDEIRRVTRAFA
CCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVGENDAIRQIAVTTNGYRLARDAANWREAGLTGV
HCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHCCHHHCCCCEE
NVSVDSLDARQFHAITGQDKFRQVMAGIDAAFDAGFEKVKVNTVLMRDVNHHQLDTFLAW
EEECCCCCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHH
IQPRPIQLRFIELMETGEGSDLFRKHHISGQVLRDELIKRGWIHQLRQRSDGPAQVFCHP
CCCCCEEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSVGKLHLCLFGDGGVSLRDLLQDDAQQYALEERI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
SDALREKKQTHFLHQSNTGITQNLSYIGG
HHHHHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA