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The map label for this gene is potH [H]

Identifier: 29142438

GI number: 29142438

Start: 2074983

End: 2075936

Strand: Reverse

Name: potH [H]

Synonym: t2017

Alternate gene names: 29142438

Gene position: 2075936-2074983 (Counterclockwise)

Preceding gene: 29142439

Following gene: 29142437

Centisome position: 43.32

GC content: 53.04

Gene sequence:

>954_bases
ATGAGCACACTTGAACCTCCGGCACGCATCAGTAAACCTGGCGGCGCGGCGCTATGGTTGGCGCGAATGCAAATGAAACA
TGGGCGTAAGCTGGTTATTGCTTTGCCTTATATCTGGCTCATTCTGCTGTTTTTACTGCCGTTTCTTATCGTCTTTAAGA
TAAGCCTGGCGGAAATGGCGCGGGCGATCCCGCCGTATACCGATCTGCTGGAATGGGCTGACGGTCAGTTGTCGATTACG
CTCAACCTCGGCAATTTTTTGCAACTGACCGACGATCCGCTCTATTTCGAAGCCTGTCTGCAATCGTTACAAATTGCCGG
TATTTCCACAATCTGCTGCCTGCTGCTGGGCTACCCGCTGGCGTGGGCGGTGGCGCACAGCAAGCCCTCCACGCGCAATA
TCCTGCTGCTGTTGGTGATTCTGCCCTCGTGGACGTCTTTTTTAATACGCGTCTATGCCTGGATGGGCATTTTGAAAAAC
AACGGTGTGCTGAACAATGTGTTGCTGTGGCTAGGGGTTATCGATCAGCCGCTGACGATCCTGCACACTAACCTGGCCGT
CTATATCGGCATTGTTTATGCGTATCTGCTGTTTATGGTTCTGCCCATTTATACCGCGTTGACGCGCATTGATTATTCGC
TGGTGGAAGCGTCGCTGGATCTCGGCGCCCGACCGATGAAAACATTTTTTAGCATCATTGTCCCGCTCACCAAAGGCGGA
ATCATCGCCGGCTCGATGCTGGTTTTTATCCCGGCGGTGGGCGAGTTCGTGATCCCGGAACTGCTTGGCGGGCCGGACAG
TATCATGATTGGTCGCGTCCTGTGGCAGGAGTTTTTCAATAACCGCGACTGGCCGGTGGCTTCTGCGGTCGCCATTATTA
TGTTGCTGCTGCTGATAGTCCCGATCATGTGGTTCCATAAACATCAGCAAAAAAGTGTGGGGGAGCACGGATGA

Upstream 100 bases:

>100_bases
TGCGCACCGTTACCGGAAAGGGTTACCGACCTGGGGCGATGAAGTGCGTTTGTGCTGGGAAGCGGATAGCTGCGTGGTCC
TGACGGTATAAGGGGGAAAC

Downstream 100 bases:

>100_bases
ATAACTTACCGGTCGTTCGCTCGCCCTGGCGCATTCTGATTCTGGTGCTGGGGTTTACTTTTCTCTATGCGCCGATGTTA
ATGTTGGTGATTTACTCGTT

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSTLEPPARISKPGGAALWLARMQMKHGRKLVIALPYIWLILLFLLPFLIVFKISLAEMARAIPPYTDLLEWADGQLSIT
LNLGNFLQLTDDPLYFEACLQSLQIAGISTICCLLLGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
NGVLNNVLLWLGVIDQPLTILHTNLAVYIGIVYAYLLFMVLPIYTALTRIDYSLVEASLDLGARPMKTFFSIIVPLTKGG
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG

Sequences:

>Translated_317_residues
MSTLEPPARISKPGGAALWLARMQMKHGRKLVIALPYIWLILLFLLPFLIVFKISLAEMARAIPPYTDLLEWADGQLSIT
LNLGNFLQLTDDPLYFEACLQSLQIAGISTICCLLLGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
NGVLNNVLLWLGVIDQPLTILHTNLAVYIGIVYAYLLFMVLPIYTALTRIDYSLVEASLDLGARPMKTFFSIIVPLTKGG
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG
>Mature_316_residues
STLEPPARISKPGGAALWLARMQMKHGRKLVIALPYIWLILLFLLPFLIVFKISLAEMARAIPPYTDLLEWADGQLSITL
NLGNFLQLTDDPLYFEACLQSLQIAGISTICCLLLGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNN
GVLNNVLLWLGVIDQPLTILHTNLAVYIGIVYAYLLFMVLPIYTALTRIDYSLVEASLDLGARPMKTFFSIIVPLTKGGI
IAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=317, Percent_Identity=91.7981072555205, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1787369, Length=254, Percent_Identity=43.7007874015748, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787713, Length=241, Percent_Identity=31.9502074688797, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35349; Mature: 35218

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLEPPARISKPGGAALWLARMQMKHGRKLVIALPYIWLILLFLLPFLIVFKISLAEMA
CCCCCCCHHCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIPPYTDLLEWADGQLSITLNLGNFLQLTDDPLYFEACLQSLQIAGISTICCLLLGYPL
HHCCCHHHHHHCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
AWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGVLNNVLLWLGVIDQPLTI
HHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
LHTNLAVYIGIVYAYLLFMVLPIYTALTRIDYSLVEASLDLGARPMKTFFSIIVPLTKGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIIMLLLLIV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
PIMWFHKHQQKSVGEHG
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STLEPPARISKPGGAALWLARMQMKHGRKLVIALPYIWLILLFLLPFLIVFKISLAEMA
CCCCCCHHCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIPPYTDLLEWADGQLSITLNLGNFLQLTDDPLYFEACLQSLQIAGISTICCLLLGYPL
HHCCCHHHHHHCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
AWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGVLNNVLLWLGVIDQPLTI
HHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
LHTNLAVYIGIVYAYLLFMVLPIYTALTRIDYSLVEASLDLGARPMKTFFSIIVPLTKGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIIMLLLLIV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
PIMWFHKHQQKSVGEHG
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]