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The map label for this gene is pflA [H]

Identifier: 29142386

GI number: 29142386

Start: 2017077

End: 2017874

Strand: Direct

Name: pflA [H]

Synonym: t1964

Alternate gene names: 29142386

Gene position: 2017077-2017874 (Clockwise)

Preceding gene: 29142385

Following gene: 29142398

Centisome position: 42.09

GC content: 48.62

Gene sequence:

>798_bases
ATGAGTAACTTAACCGATTGTATTACAAATGAAAGTGTTGCAGTAACTGCTGATAAAAAGCCGGTTATTGGTCGCATTCA
CTCCTTTGAGTCCTGTGGCACCGTTGATGGCCCAGGCATCCGCTTTATTACCTTTTTCCAGGGCTGCCTGATGCGCTGCC
TGTATTGCCATAACCGCGACACATGGGATACGCATGGCGGTAAAGAAATCACCGTTGAAGACTTAATGAAAGAGGTGGTG
ACCTATCGGCATTTTATGAATGCTTCTGGCGGCGGCGTGACGGCATCCGGCGGCGAGGCCATTTTGCAGGCAGAATTTGT
ACGCGACTGGTTTCGCGCCTGTAAAAAAGAAGGTATCCACACCTGTCTCGACACCAACGGCTTTGTGCGCCGTTATGACC
CGGTGATTGATGAACTGTTGGACGTTACCGATCTGGTGATGCTCGATCTCAAGCAGATGAATGATGAGATCCACCAGAAT
CTGGTCGGTGTATCCAATCACCGGACGCTGGAATTCGCGCAGTATTTATCAAAGAAAAATGTGAAGGTCTGGATCCGTTA
CGTTGTCGTTCCCGGCTGGTCTGACGACGATGATTCCGCGCATCGCCTGGGTGAGTTTACCCGCGATATGGGTAATGTCG
AAAAAATCGAACTGCTGCCCTATCATGAGCTGGGCAAACATAAATGGGTGGCAATGGGCGAAGAATATAAACTGGATGGC
GTGAAGCCGCCTAAAAAAGAGACCATGGAACGTGTAAAGGGTATTCTTGAGCAGTATGGTCATAAAGTGATGTATTAA

Upstream 100 bases:

>100_bases
AGTACCGCTTTTTTACAGGATGCAACCCCCTTACTAGGGTGCTCAACATGCCCGGAATGTGTCCATAAACCCCATGTTAT
TTAGAGCTAGCCGAAGCGAT

Downstream 100 bases:

>100_bases
TCGCCTGCTATCTTATCCGCTTTTAAACGGCTACCCATGACGGTAGCCGTTTTGCTGTCTGGGTAACGCTCTTACCGCTA
AAATACCCGACCCCGCCGTC

Product: pyruvate formate-lyase activating protein 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSNLTDCITNESVAVTADKKPVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEDLMKEVV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDEIHQN
LVGVSNHRTLEFAQYLSKKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
VKPPKKETMERVKGILEQYGHKVMY

Sequences:

>Translated_265_residues
MSNLTDCITNESVAVTADKKPVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEDLMKEVV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDEIHQN
LVGVSNHRTLEFAQYLSKKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
VKPPKKETMERVKGILEQYGHKVMY
>Mature_264_residues
SNLTDCITNESVAVTADKKPVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEDLMKEVVT
YRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDEIHQNL
VGVSNHRTLEFAQYLSKKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGV
KPPKKETMERVKGILEQYGHKVMY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=244, Percent_Identity=97.5409836065574, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1790389, Length=285, Percent_Identity=25.9649122807018, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790839, Length=286, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI226510931, Length=171, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30252; Mature: 30121

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLTDCITNESVAVTADKKPVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRD
CCCHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TWDTHGGKEITVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIH
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCC
TCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLSKKN
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
VKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
EEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCC
VKPPKKETMERVKGILEQYGHKVMY
CCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNLTDCITNESVAVTADKKPVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRD
CCHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TWDTHGGKEITVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIH
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCC
TCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLSKKN
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
VKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
EEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCC
VKPPKKETMERVKGILEQYGHKVMY
CCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]