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The map label for this gene is focA [H]

Identifier: 29142383

GI number: 29142383

Start: 2012271

End: 2013128

Strand: Direct

Name: focA [H]

Synonym: t1960

Alternate gene names: 29142383

Gene position: 2012271-2013128 (Clockwise)

Preceding gene: 29142382

Following gene: 29142384

Centisome position: 41.99

GC content: 48.25

Gene sequence:

>858_bases
GTGAAAGCTGACAACCCTTTTGACCTTTTACTCCCTGCAGCAATGGCCAAAGTGGCTGAAGAAGCAGGCGTCTATAAAGC
AACGAAACATCCGCTTAAGACTTTCTATCTGGCGATTACCGCCGGCGTATTCATTTCAATTGCCTTTGTTTTTTATATTA
CTGCGACAACCGGTACTGGCGCGATGCCTTATGGCATGGCGAAACTTATCGGGGGTATCTGTTTCTCTTTGGGACTGATT
CTCTGTGTTATTTGCGGCGCTGATCTTTTCACCTCTACTGTTCTGATCGTTGTAGCTAAAGCTAGCGGTCGCATCACCTG
GGGACAACTGGCTAAAAACTGGTTAAACGTCTATTTTGGTAACCTGATTGGCGCTCTGCTTTTCGTGTTACTGATGTGGC
TTTCCGGCGAGTATATGACTGCCAACGGTCAATGGGGACTTAACGTCCTGCAAACCGCCGACCACAAAATGCACCACACT
TTTATTGAAGCTGTCTGCCTCGGTATTCTGGCGAACCTGATGGTTTGTCTGGCCGTGTGGATGAGCTACTCAGGTCGTAG
CCTGATGGACAAAGCGTTCATCATGGTACTGCCCGTCGCCATGTTTGTCGCCAGCGGTTTTGAGCACAGTATCGCAAACA
TGTTTATGATTCCTATGGGTATTGTAATACGCGATTTCGCTACACCGGAGTTTTGGACCGCGGTTGGTTCGTCTCCGGAA
AGTTTTTCTCACCTGACCGTGATGAGCTTCATCACTGATAACCTGATTCCGGTCACGATCGGTAATATTATCGGCGGCGG
TTTGTTGGTCGGGTTGACATACTGGGTCATTTACCTGCGTGGTAACGAGCATCATTAA

Upstream 100 bases:

>100_bases
TTACGGGCCTATAAGCCAGGCGAGATATGATCTATATCAAATTCTCATCTATAATGCTTTGTTAGTATCTCGTCGCCGAC
TTAATAAAGAGAGAGTTAGT

Downstream 100 bases:

>100_bases
TCTGTTGATGTCCATTACACGCAGTAAATAAAAAATCCACTTAAGAAGGTAGGTGTTACATGTCCGAGCTTAATGAAAAG
TTAGCCACAGCCTGGGAAGG

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGICFSLGLI
LCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPE
SFSHLTVMSFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH

Sequences:

>Translated_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGICFSLGLI
LCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPE
SFSHLTVMSFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH
>Mature_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGICFSLGLI
LCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPE
SFSHLTVMSFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=95.7894736842105, Blast_Score=562, Evalue=1e-162,
Organism=Escherichia coli, GI1788837, Length=280, Percent_Identity=50.3571428571429, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI87082249, Length=268, Percent_Identity=27.6119402985075, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=207, Percent_Identity=28.5024154589372, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30969; Mature: 30969

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMPYGMAKLIGGICFSLGLILCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
NLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPE
HCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
SFSHLTVMSFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMPYGMAKLIGGICFSLGLILCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
NLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPE
HCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
SFSHLTVMSFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]