Click here to switch to the map view.

The map label for this gene is dam [H]

Identifier: 29142351

GI number: 29142351

Start: 1969578

End: 1970459

Strand: Direct

Name: dam [H]

Synonym: t1926

Alternate gene names: 29142351

Gene position: 1969578-1970459 (Clockwise)

Preceding gene: 29142350

Following gene: 29142352

Centisome position: 41.1

GC content: 53.06

Gene sequence:

>882_bases
ATGAGATACGGCTCTGTTTGCAGTGGAACTGCGGCGATGCCCATAGAGGAACCGGCGCTGCGCACCTGGCGCCGCCCGTT
CCTGAAATGGGCAGGCGGTAAATATTCGCTGCTGCCGGAACTGGATCGTCTTATCCCGGCAGGTAAACGACTGATAGAGC
CATTTGTGGGCGGCGGATCGGTGTTTCTTAACTCAGACAAGCACGAACGCTTCCTTCTGGCTGACGTCAACGCTGACCTG
ATTAACCTGTACCAGATGCTGGCCGTAGTGCCTGATTCGGTAATCGCAGAGGCTATGAAAGCTTTCAGACATCTGAATGA
TGCCGAAAACTACACAGTAATTCGTGAAGCATTCAACGCCCAGAAACTGAATGCGACAGAACGAGCGGCCGCATTCCTTT
ACCTCAACAGGCACTGCTTTAACGGTCTGATGCGTTACAACCTTGACGGTTTTTTCAATGTTGGATGGGGAAAGTATAAA
GCCCCATATTTCCCGGAAGAAGAGATCAGGGCATTCAGGCAGAAGTCTCACGCGTGCGTATTTATGACTGCGGGTTTCGA
ACGTACTCTCAGGCTGGCGGGTGATGGTGATGTCGTTTACTGCGATCCGCCATACGAGCCAATGCCCGGCACCGCTGGCT
TCACTAACTACGCCTCCGGTGGGTTCTCATGGGATAGCCAGGTAGCGCTTGCTGAAAGCTGTGTGGCAGCCCATCAGCGT
GGCGCAAAGGTGTTTATCAGTAATTCTACCGCACCACGCGTTATTGAACTTTACGAGAGGCACGGCTTCACTCTGCACCG
GGTCAATGCCCGCAGATCAATATCGAGTAAAGGCAGTACCCGAGAAACAGCGAACGATATCGTCGCCTCACTGGGGATTT
AG

Upstream 100 bases:

>100_bases
AAAAAGATAGAGCGACAGTTATTGCCAGCGTTGCTTCACAAAAAGCTGCTTCGGTGAGACTAATCCAAGCTATTACGACA
AAGAGCTTTGAGGTCGCAGC

Downstream 100 bases:

>100_bases
TGATGATGAAACTTATTAACAGAAGTAAGCAATCACCAGTCGGTCGTCGCGCATGTGATATTGCACTGGCTGCGCATCAT
GAGAAGTTCGGCGATTACGG

Product: DNA methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRYGSVCSGTAAMPIEEPALRTWRRPFLKWAGGKYSLLPELDRLIPAGKRLIEPFVGGGSVFLNSDKHERFLLADVNADL
INLYQMLAVVPDSVIAEAMKAFRHLNDAENYTVIREAFNAQKLNATERAAAFLYLNRHCFNGLMRYNLDGFFNVGWGKYK
APYFPEEEIRAFRQKSHACVFMTAGFERTLRLAGDGDVVYCDPPYEPMPGTAGFTNYASGGFSWDSQVALAESCVAAHQR
GAKVFISNSTAPRVIELYERHGFTLHRVNARRSISSKGSTRETANDIVASLGI

Sequences:

>Translated_293_residues
MRYGSVCSGTAAMPIEEPALRTWRRPFLKWAGGKYSLLPELDRLIPAGKRLIEPFVGGGSVFLNSDKHERFLLADVNADL
INLYQMLAVVPDSVIAEAMKAFRHLNDAENYTVIREAFNAQKLNATERAAAFLYLNRHCFNGLMRYNLDGFFNVGWGKYK
APYFPEEEIRAFRQKSHACVFMTAGFERTLRLAGDGDVVYCDPPYEPMPGTAGFTNYASGGFSWDSQVALAESCVAAHQR
GAKVFISNSTAPRVIELYERHGFTLHRVNARRSISSKGSTRETANDIVASLGI
>Mature_293_residues
MRYGSVCSGTAAMPIEEPALRTWRRPFLKWAGGKYSLLPELDRLIPAGKRLIEPFVGGGSVFLNSDKHERFLLADVNADL
INLYQMLAVVPDSVIAEAMKAFRHLNDAENYTVIREAFNAQKLNATERAAAFLYLNRHCFNGLMRYNLDGFFNVGWGKYK
APYFPEEEIRAFRQKSHACVFMTAGFERTLRLAGDGDVVYCDPPYEPMPGTAGFTNYASGGFSWDSQVALAESCVAAHQR
GAKVFISNSTAPRVIELYERHGFTLHRVNARRSISSKGSTRETANDIVASLGI

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=266, Percent_Identity=45.8646616541353, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32665; Mature: 32665

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYGSVCSGTAAMPIEEPALRTWRRPFLKWAGGKYSLLPELDRLIPAGKRLIEPFVGGGS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
VFLNSDKHERFLLADVNADLINLYQMLAVVPDSVIAEAMKAFRHLNDAENYTVIREAFNA
EEEECCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
QKLNATERAAAFLYLNRHCFNGLMRYNLDGFFNVGWGKYKAPYFPEEEIRAFRQKSHACV
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEE
FMTAGFERTLRLAGDGDVVYCDPPYEPMPGTAGFTNYASGGFSWDSQVALAESCVAAHQR
EEECCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GAKVFISNSTAPRVIELYERHGFTLHRVNARRSISSKGSTRETANDIVASLGI
CCEEEEECCCCCHHHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRYGSVCSGTAAMPIEEPALRTWRRPFLKWAGGKYSLLPELDRLIPAGKRLIEPFVGGGS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
VFLNSDKHERFLLADVNADLINLYQMLAVVPDSVIAEAMKAFRHLNDAENYTVIREAFNA
EEEECCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
QKLNATERAAAFLYLNRHCFNGLMRYNLDGFFNVGWGKYKAPYFPEEEIRAFRQKSHACV
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEE
FMTAGFERTLRLAGDGDVVYCDPPYEPMPGTAGFTNYASGGFSWDSQVALAESCVAAHQR
EEECCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GAKVFISNSTAPRVIELYERHGFTLHRVNARRSISSKGSTRETANDIVASLGI
CCEEEEECCCCCHHHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940 [H]