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The map label for this gene is rlmL [H]

Identifier: 29142286

GI number: 29142286

Start: 1919781

End: 1921889

Strand: Reverse

Name: rlmL [H]

Synonym: t1859

Alternate gene names: 29142286

Gene position: 1921889-1919781 (Counterclockwise)

Preceding gene: 29142288

Following gene: 29142285

Centisome position: 40.11

GC content: 54.81

Gene sequence:

>2109_bases
ATGAATTCTCTGTTTGCCAGTACGGCCCGCGGGCTGGAAGAGCTGTTAAAAATTGAACTGGAAAAACTGGGCGCCGTAGG
GTGTCAGGTTGTTCAGGGTGGGGTCCATTTTCAGGGCGACACGCGGCTTATTTACCAGAGCCTGATGTGGAGCCGCCTGG
CCTCGCGTATTATCCTGCCGATGGGTGAGTGCAAGGTCTACAGCGATCTCGATCTTTACTTCGGCGTTCAGGCGATAAAC
TGGACAGAGATTTTTAATCCTGGCGCGACGTTTGCGGTGCATTTCAGCGGCCTGAACGACACCATCCGTAATAGTCAGTA
TGGCGCGATGAAAGTTAAAGACGCGATTGTCGATGCGTTTACGCGGAAAAATCTTCCGCGTCCAAATGTGGACCGCGAAT
CGCCGGACCTCCGTATTAACGTCTGGCTGAATAAAGAAACGGCCAGCATTGCGCTGGATCTCAGTGGTGATGGCCTGCAC
CTGCGCGGTTATCGCGATCGCACCGGGCTGGCGCCAATTAAAGAGACGCTGGCGGCAGCGATTGTTATGCGTTCCGGCTG
GCAACCAGGTACGCCGCTGCTCGACCCTATGTGTGGTTCGGGGACACTGTTGATTGAGGCCGCGATGTGGGCGACCGATC
GCGCGCCGGGCCTGCATCGTGGTCACTGGGGATTTAGCGGCTGGGCGCAGCATGACGAGGCTATCTGGCAGGAAGTGAAA
GCCGAAGCGCAGACCCGGGCGCGTAAGGGGCTGGCGGAGTATTCCTCTCATTTCTACGGTTCCGACAGCGATGCGCGCGT
CATTGAGCGGGCGCGCAGCAACGCCCGTCGCGCCGGTATCGGGGAGCTTATCACTTTTGAAGTGAAAGATGTGGCGCAAC
TGAGTAATCCATTGCCGAAAGGCCCGTATGGTACGGTGATCAGTAACCCGCCTTACGGCGAACGTCTGGACAGCGATCCG
GCGCTGATTGCGTTGCACAGCCTGTTGGGCCGTACCATGAAAAATCAGTTTGGCGGCTGGAACCTGTCGCTGTTCAGCGC
TTCGCCCGATTTGCTGGGCAGCCTGCAATTACGCGCCGACAAACAGTTTAAAGCCAAGAATGGCCCGCTGGACTGTGTGC
AGAAAAACTATCATATTGCGGAAACCACGGCGGACAGTAAACCGGCGACGGTGGCGGAAGATTACGCTAATCGCCTGCGT
AAAAATCTTAAAAAGCTGGAGAAATGGGCGCGCCAGGAAGGGATTGAATGCTATCGCTTGTATGATGCCGACCTGCCGGA
ATATAACGTGGCGGTAGACCGTTACGGCGACTGGGCGGTGATTCAGGAATATGCGCCGCCGAAAACGGTGGATGCGCAAA
AAGCGCGGCAACGTCTGTTCGATATTATTGCGGCAACCTTGTCAGTGCTCGGTATTCCGCCGAATAAACTGGTGTTAAAA
ACCCGCGAACGGCAAAAAGGAAAAAACCAGTATCAGAAAATGAGCGAGAAGGGCGAGTTCCTTGAGGTCAGCGAATATAA
TGCGCGCTTATGGGTAAATCTGACGGATTATCTCGACACTGGTCTGTTCCTCGATCACCGTATTGCCCGCAGAATGCTGG
GAGAAATGAGCAAAGGCAAAGATTTCCTGAATCTCTTTTCTTATACCGGCAGCGCCAGCGTACATGCGGGTCTGGGCGGC
GCGCGCAACACCACAACGGTGGATATGTCCCGCACCTATCTGGAGTGGGCCGAACGTAATTTGCGTCTGAACGGACTGAG
CGGCCGCGCGCATCGCCTGATTCAGGCCGACTGCCTGGGCTGGCTGCGCGAGGCGAATGAACAGTTCGATTTGATCTTTA
TCGATCCGCCGACGTTCTCTAACTCAAAACGTATGGAAGAGTCGTTTGATGTTCAGCGCGATCATGTCGCGTTAATGAAA
GATTTAAAACGCCTGCTGCGTAAAGGCGGCACCATCATGTTCTCAAATAATAAGCGCGGATTCCGTATGGATCTGGAAGG
GCTGGCTGAGCTGGGGCTTACCGCACAAGAAATTACGCAAAAAACGCTTTCCCCGGATTTTGCCCGTAACCGTCAGATTC
ATAATTGCTGGCTGATCCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
ACCGTACAGTCCCTCAAATGAAAATAAGCCTTCAACTTTATGACATGGGCGTGAGATTAGCTATAATGCGCAACAATTTT
CTTAGCGTAAAAGTGACAAT

Downstream 100 bases:

>100_bases
AAGGAAAAGTAAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGA
GTTGCATATCGAAGATAACG

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MNSLFASTARGLEELLKIELEKLGAVGCQVVQGGVHFQGDTRLIYQSLMWSRLASRIILPMGECKVYSDLDLYFGVQAIN
WTEIFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRESPDLRINVWLNKETASIALDLSGDGLH
LRGYRDRTGLAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMWATDRAPGLHRGHWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIERARSNARRAGIGELITFEVKDVAQLSNPLPKGPYGTVISNPPYGERLDSDP
ALIALHSLLGRTMKNQFGGWNLSLFSASPDLLGSLQLRADKQFKAKNGPLDCVQKNYHIAETTADSKPATVAEDYANRLR
KNLKKLEKWARQEGIECYRLYDADLPEYNVAVDRYGDWAVIQEYAPPKTVDAQKARQRLFDIIAATLSVLGIPPNKLVLK
TRERQKGKNQYQKMSEKGEFLEVSEYNARLWVNLTDYLDTGLFLDHRIARRMLGEMSKGKDFLNLFSYTGSASVHAGLGG
ARNTTTVDMSRTYLEWAERNLRLNGLSGRAHRLIQADCLGWLREANEQFDLIFIDPPTFSNSKRMEESFDVQRDHVALMK
DLKRLLRKGGTIMFSNNKRGFRMDLEGLAELGLTAQEITQKTLSPDFARNRQIHNCWLIRAA

Sequences:

>Translated_702_residues
MNSLFASTARGLEELLKIELEKLGAVGCQVVQGGVHFQGDTRLIYQSLMWSRLASRIILPMGECKVYSDLDLYFGVQAIN
WTEIFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRESPDLRINVWLNKETASIALDLSGDGLH
LRGYRDRTGLAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMWATDRAPGLHRGHWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIERARSNARRAGIGELITFEVKDVAQLSNPLPKGPYGTVISNPPYGERLDSDP
ALIALHSLLGRTMKNQFGGWNLSLFSASPDLLGSLQLRADKQFKAKNGPLDCVQKNYHIAETTADSKPATVAEDYANRLR
KNLKKLEKWARQEGIECYRLYDADLPEYNVAVDRYGDWAVIQEYAPPKTVDAQKARQRLFDIIAATLSVLGIPPNKLVLK
TRERQKGKNQYQKMSEKGEFLEVSEYNARLWVNLTDYLDTGLFLDHRIARRMLGEMSKGKDFLNLFSYTGSASVHAGLGG
ARNTTTVDMSRTYLEWAERNLRLNGLSGRAHRLIQADCLGWLREANEQFDLIFIDPPTFSNSKRMEESFDVQRDHVALMK
DLKRLLRKGGTIMFSNNKRGFRMDLEGLAELGLTAQEITQKTLSPDFARNRQIHNCWLIRAA
>Mature_702_residues
MNSLFASTARGLEELLKIELEKLGAVGCQVVQGGVHFQGDTRLIYQSLMWSRLASRIILPMGECKVYSDLDLYFGVQAIN
WTEIFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRESPDLRINVWLNKETASIALDLSGDGLH
LRGYRDRTGLAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMWATDRAPGLHRGHWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIERARSNARRAGIGELITFEVKDVAQLSNPLPKGPYGTVISNPPYGERLDSDP
ALIALHSLLGRTMKNQFGGWNLSLFSASPDLLGSLQLRADKQFKAKNGPLDCVQKNYHIAETTADSKPATVAEDYANRLR
KNLKKLEKWARQEGIECYRLYDADLPEYNVAVDRYGDWAVIQEYAPPKTVDAQKARQRLFDIIAATLSVLGIPPNKLVLK
TRERQKGKNQYQKMSEKGEFLEVSEYNARLWVNLTDYLDTGLFLDHRIARRMLGEMSKGKDFLNLFSYTGSASVHAGLGG
ARNTTTVDMSRTYLEWAERNLRLNGLSGRAHRLIQADCLGWLREANEQFDLIFIDPPTFSNSKRMEESFDVQRDHVALMK
DLKRLLRKGGTIMFSNNKRGFRMDLEGLAELGLTAQEITQKTLSPDFARNRQIHNCWLIRAA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=91.5954415954416, Blast_Score=1335, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=241, Percent_Identity=29.045643153527, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 78879; Mature: 78879

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKIELEKLGAVGCQVVQGGVHFQGDTRLIYQSLMWSRLASRIILP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
MGECKVYSDLDLYFGVQAINWTEIFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEEECCHHHCCEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRESPDLRINVWLNKETASIALDLSGDGLHLRGYRDRTGLAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHH
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMWATDRAPGLHRGHWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIERARSNARRAGIGELITFEVKDVAQLSNPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCC
GPYGTVISNPPYGERLDSDPALIALHSLLGRTMKNQFGGWNLSLFSASPDLLGSLQLRAD
CCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEECCC
KQFKAKNGPLDCVQKNYHIAETTADSKPATVAEDYANRLRKNLKKLEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYGDWAVIQEYAPPKTVDAQKARQRLFDIIAATLSVLGIPPNKLVLK
ECCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERQKGKNQYQKMSEKGEFLEVSEYNARLWVNLTDYLDTGLFLDHRIARRMLGEMSKGK
CHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSASVHAGLGGARNTTTVDMSRTYLEWAERNLRLNGLSGRAHRLIQADCLG
HHHHHHHCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEESFDVQRDHVALMKDLKRLLRKGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
FRMDLEGLAELGLTAQEITQKTLSPDFARNRQIHNCWLIRAA
EEECHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MNSLFASTARGLEELLKIELEKLGAVGCQVVQGGVHFQGDTRLIYQSLMWSRLASRIILP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
MGECKVYSDLDLYFGVQAINWTEIFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEEECCHHHCCEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRESPDLRINVWLNKETASIALDLSGDGLHLRGYRDRTGLAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHH
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMWATDRAPGLHRGHWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIERARSNARRAGIGELITFEVKDVAQLSNPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCC
GPYGTVISNPPYGERLDSDPALIALHSLLGRTMKNQFGGWNLSLFSASPDLLGSLQLRAD
CCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEECCC
KQFKAKNGPLDCVQKNYHIAETTADSKPATVAEDYANRLRKNLKKLEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYGDWAVIQEYAPPKTVDAQKARQRLFDIIAATLSVLGIPPNKLVLK
ECCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERQKGKNQYQKMSEKGEFLEVSEYNARLWVNLTDYLDTGLFLDHRIARRMLGEMSKGK
CHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSASVHAGLGGARNTTTVDMSRTYLEWAERNLRLNGLSGRAHRLIQADCLG
HHHHHHHCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEESFDVQRDHVALMKDLKRLLRKGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
FRMDLEGLAELGLTAQEITQKTLSPDFARNRQIHNCWLIRAA
EEECHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA