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The map label for this gene is hpaB [H]

Identifier: 29142250

GI number: 29142250

Start: 1883324

End: 1884631

Strand: Direct

Name: hpaB [H]

Synonym: t1820

Alternate gene names: 29142250

Gene position: 1883324-1884631 (Clockwise)

Preceding gene: 29142249

Following gene: 29142251

Centisome position: 39.3

GC content: 53.44

Gene sequence:

>1308_bases
GTGGCGAAAAGCGCAGACGATCTGCGCCAACAGCGTGATGCTATCGCCGAGTGGTCACGCCTGAGTTACGGCTGGATGGG
ACGCACACCGGATTACAAAGCCGCCTTTGGCTGCGCTCTGGGCGCTAACCCAGCCTTCTACGGCCAGTTTGAGCAGAACG
CCCGTAACTGGTACACCCGTATTCAGGAGACCGGCCTGTACTTTAACCATGCAATCGTCAACCCGCCCATTGACCGCCAC
AAACCTGCCGACGAAGTGAAAGACGTCTATATCAAGCTGGAGAAAGAGACGGACGCCGGGATTATTGTCAGCGGGGCGAA
AGTTGTCGCCACTAACTCCGCCCTGACTCACTACAACATGATTGGTTTCGGCTCAGCCCAGGTGATGGGCGAAAACCCGG
ATTTTGCTCTGATGTTTGTCGCGCCAATGGATGCCGAAGGCGTAAAACTTATTTCGCGCGCCTCGTATGAAATGGTCGCG
GGCGCGACGGGCTCGCCGTTTGATTATCCCCTCTCCAGCCGTTTTGATGAAAACGATGCCATTCTGGTGATGGACAAGGT
GCTGATCCCGTGGGAAAACGTATTAATTTACCGTGATTTCGATCGTTGTCGTCGCTGGACGATGGAAGGCGGCTTTGCCC
GTATGTATCCACTGCAAGCCTGTGTTCGTCTGGCGGTAAAACTTGATTTCATTACCGCGCTGCTGAAAAAATCGCTCGAA
TGTACGGGTACCGTAGAGTTCCGGGGCGTGCAGGCCGATCTCGGCGAAGTCGTGGCCTGGCGCAATATGTTCTGGGCATT
GAGCGATTCTATGTGTTCTGAAGCAACCCCGTGGGTAAACGGCGCCTGGCTACCGGACCACGCCGCGCTGCAAACCTATC
GTGTGATGGCCCCAATGGCCTACGCGAAAATTAAAAATATTATTGAACGTAACGTTACCAGCGGCCTGATTTACCTGCCT
TCCAGCGCCCGCGATCTGAATAATCCGCAAATCGACCAGTACCTGGCGAAATACGTACGCGGCTCTAACGGAATGGACCA
TGTTGAACGTATCAAAATTCTTAAATTGATGTGGGATGCCATCGGCAGCGAGTTTGGCGGTCGCCATGAGCTGTACGAGA
TTAACTACTCGGGCAGCCAGGATGAAATTCGTCTGCAGTGTCTGCGTCAGGCCCAGAGCTCCGGCAATATGGATAAGATG
ATGGCAATGGTCGATCGCTGCCTCTCCGAATACGATCAGAATGGCTGGACGGTTTCGCATTTGCACAATAACGACGACAT
CAATCAACTGGATAAGCTGCTGAAATAA

Upstream 100 bases:

>100_bases
TTACCTTCCCACGCCGCCGACACGGGAATCCCCTCCCGCTTACACCCTGTGCTGGAATACCGATACCGGCAGCGGCGGTT
ATACGCATAAATTTTTCCGC

Downstream 100 bases:

>100_bases
CGCGGCAGGAGGTTCTCATGCAAGTAGATGAACAACGTCTGCGTTTTCGCGATGCGATGGCAAGTCTGGCGGCAGCGGTC
AACATCGTAACCACGGCGGG

Product: 4-hydroxyphenylacetate 3-monooxygenase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPAFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRH
KPADEVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDAEGVKLISRASYEMVA
GATGSPFDYPLSSRFDENDAILVMDKVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLE
CTGTVEFRGVQADLGEVVAWRNMFWALSDSMCSEATPWVNGAWLPDHAALQTYRVMAPMAYAKIKNIIERNVTSGLIYLP
SSARDLNNPQIDQYLAKYVRGSNGMDHVERIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKM
MAMVDRCLSEYDQNGWTVSHLHNNDDINQLDKLLK

Sequences:

>Translated_435_residues
MAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPAFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRH
KPADEVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDAEGVKLISRASYEMVA
GATGSPFDYPLSSRFDENDAILVMDKVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLE
CTGTVEFRGVQADLGEVVAWRNMFWALSDSMCSEATPWVNGAWLPDHAALQTYRVMAPMAYAKIKNIIERNVTSGLIYLP
SSARDLNNPQIDQYLAKYVRGSNGMDHVERIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKM
MAMVDRCLSEYDQNGWTVSHLHNNDDINQLDKLLK
>Mature_434_residues
AKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPAFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRHK
PADEVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDAEGVKLISRASYEMVAG
ATGSPFDYPLSSRFDENDAILVMDKVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLEC
TGTVEFRGVQADLGEVVAWRNMFWALSDSMCSEATPWVNGAWLPDHAALQTYRVMAPMAYAKIKNIIERNVTSGLIYLPS
SARDLNNPQIDQYLAKYVRGSNGMDHVERIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMM
AMVDRCLSEYDQNGWTVSHLHNNDDINQLDKLLK

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 49195; Mature: 49063

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPAFYGQFEQNARNWYTR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHH
IQETGLYFNHAIVNPPIDRHKPADEVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNM
HHHCCCEEEEEECCCCCCCCCCHHHHCEEEEEEECCCCCCEEEECCEEEEECCCHHEEEE
IGFGSAQVMGENPDFALMFVAPMDAEGVKLISRASYEMVAGATGSPFDYPLSSRFDENDA
EECCCCEEECCCCCEEEEEEECCCCCHHHHHHHCCHHHEECCCCCCCCCCCCCCCCCCCE
ILVMDKVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLE
EEEEEEEECCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
CTGTVEFRGVQADLGEVVAWRNMFWALSDSMCSEATPWVNGAWLPDHAALQTYRVMAPMA
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
YAKIKNIIERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVERIKILKLMWDA
HHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVSH
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
LHNNDDINQLDKLLK
ECCCCCHHHHHHHHC
>Mature Secondary Structure 
AKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPAFYGQFEQNARNWYTR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHH
IQETGLYFNHAIVNPPIDRHKPADEVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNM
HHHCCCEEEEEECCCCCCCCCCHHHHCEEEEEEECCCCCCEEEECCEEEEECCCHHEEEE
IGFGSAQVMGENPDFALMFVAPMDAEGVKLISRASYEMVAGATGSPFDYPLSSRFDENDA
EECCCCEEECCCCCEEEEEEECCCCCHHHHHHHCCHHHEECCCCCCCCCCCCCCCCCCCE
ILVMDKVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLE
EEEEEEEECCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
CTGTVEFRGVQADLGEVVAWRNMFWALSDSMCSEATPWVNGAWLPDHAALQTYRVMAPMA
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
YAKIKNIIERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVERIKILKLMWDA
HHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVSH
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
LHNNDDINQLDKLLK
ECCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]