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The map label for this gene is sifA [H]

Identifier: 29142130

GI number: 29142130

Start: 1760844

End: 1761854

Strand: Direct

Name: sifA [H]

Synonym: t1696

Alternate gene names: 29142130

Gene position: 1760844-1761854 (Clockwise)

Preceding gene: 29142129

Following gene: 29142131

Centisome position: 36.75

GC content: 40.55

Gene sequence:

>1011_bases
ATGCCGATTACTATAGGGAATGGTTTTTTAAAAAGTGAAATCCTTACCAACTCCCCAAGGAATACGAAAGAAGCATGGTG
GAAAGTTTTATGGGAAAAAATTAAAGACTTCTTTTTTTCTACTGGCAAAGCAAAAGCGGACCGTTGTCTACATGAGATGT
TGTTTGCCGATCGCACCCCCACACGAGAGCGATTTACAGAGATTTTTTTTGAGTTAAAAGAGTTAGCCTGCGCATCGCAA
AGAGATAGATTTCAGGTTCATAATCCTCATGAAAATGATGACACGATTATTCTTCGCATCATGGATCAAAACGAAGAAAA
CGAATTGTTACGTATCACTCAAAATACCGATACCTTTAGCTGTGAAGTCATGGGGAATGCTTATTTTTTAATAAAAGATC
GCCCCGATATTTTAAAACCGCATCCACAAATGACGGCCATGATTAATAGAAGATATAGCGAAATCGTAGATTACCCCCTC
CCTTCGACATTATGTCTCAACCCTGCTGGCGCGCCGACATTATCGGTTCCATTAGACAACATAGAGGGGTATTTATATTC
TGAATGGAGAAAAGGAAATTTAGATGAGTGGAAAACGCAAGAAAAGGCAACCTACCTGGCAGCGAAAATTCAGTCTGGGA
TTGAAAAGACAACGCGCATTTTACAGCATGCGAATATATCCGAAAGTACTCAGCAAAACGCATTTTTAGAAACAATGACG
ATGTGTGGCTTAAAACAGCTTGAAATACCACCACCACATACCCACATACCTATTGAAAAAATGGTAGAAGAGGTTTTACT
GGCGGATAAGACGTTTCAGGCGTTCCTTGTAACGGATCCCAGCGCCAGCCTAAGCATGTTAGCTGAGATAGTCGAAGCCA
TCTCTGATCAGGTTTTTCACGCCATTTTTAGAATAGCCCCCCAGTCTATACAAAAAATGGCGGAAGAACAGTTAACCACG
CTACACGTTCGCTCAGAACAACAAAGCGGCTATTTATGTTGTTTTTTATAA

Upstream 100 bases:

>100_bases
TTAAAAATTATATTACATCCGATGCGCCCGCAGTTGAGATAAAAAGAGTTGATTTAATCAATTATGTAGTCATTTTTACT
CCAGTATAAGTGAGATTAAT

Downstream 100 bases:

>100_bases
AATCAGACGACGCTTTCTCAGACGTCACGTTCAGGGTTTACTCACGTACTTCATGTTTGCCTACAGAGTAACCTCTTGCC
TGGCGCAATAATGCCGCATA

Product: secreted effector protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFADRTPTRERFTEIFFELKELACASQ
RDRFQVHNPHENDDTIILRIMDQNEENELLRITQNTDTFSCEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPL
PSTLCLNPAGAPTLSVPLDNIEGYLYSEWRKGNLDEWKTQEKATYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
MCGLKQLEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIVEAISDQVFHAIFRIAPQSIQKMAEEQLTT
LHVRSEQQSGYLCCFL

Sequences:

>Translated_336_residues
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFADRTPTRERFTEIFFELKELACASQ
RDRFQVHNPHENDDTIILRIMDQNEENELLRITQNTDTFSCEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPL
PSTLCLNPAGAPTLSVPLDNIEGYLYSEWRKGNLDEWKTQEKATYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
MCGLKQLEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIVEAISDQVFHAIFRIAPQSIQKMAEEQLTT
LHVRSEQQSGYLCCFL
>Mature_335_residues
PITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFADRTPTRERFTEIFFELKELACASQR
DRFQVHNPHENDDTIILRIMDQNEENELLRITQNTDTFSCEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPLP
STLCLNPAGAPTLSVPLDNIEGYLYSEWRKGNLDEWKTQEKATYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMTM
CGLKQLEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIVEAISDQVFHAIFRIAPQSIQKMAEEQLTTL
HVRSEQQSGYLCCFL

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella- induced filaments (Sifs), which are filamentous structures containing l

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cytoplasm. Host cell membrane. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Sif family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010637 [H]

Pfam domain/function: PF06767 Sif [H]

EC number: NA

Molecular weight: Translated: 38664; Mature: 38533

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFADRTP
CCEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
TRERFTEIFFELKELACASQRDRFQVHNPHENDDTIILRIMDQNEENELLRITQNTDTFS
CHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEE
CEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPLPSTLCLNPAGAPTLSVPLDN
EEEECCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCHHH
IEGYLYSEWRKGNLDEWKTQEKATYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MCGLKQLEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIVEAISDQVFH
HCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
AIFRIAPQSIQKMAEEQLTTLHVRSEQQSGYLCCFL
HHHHHCHHHHHHHHHHHHHEEEEECCCCCCCEEEEC
>Mature Secondary Structure 
PITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFADRTP
CEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
TRERFTEIFFELKELACASQRDRFQVHNPHENDDTIILRIMDQNEENELLRITQNTDTFS
CHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEE
CEVMGNAYFLIKDRPDILKPHPQMTAMINRRYSEIVDYPLPSTLCLNPAGAPTLSVPLDN
EEEECCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCHHH
IEGYLYSEWRKGNLDEWKTQEKATYLAAKIQSGIEKTTRILQHANISESTQQNAFLETMT
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MCGLKQLEIPPPHTHIPIEKMVEEVLLADKTFQAFLVTDPSASLSMLAEIVEAISDQVFH
HCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
AIFRIAPQSIQKMAEEQLTTLHVRSEQQSGYLCCFL
HHHHHCHHHHHHHHHHHHHEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8861213; 11677609; 10861017 [H]