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The map label for this gene is rluB

Identifier: 29142117

GI number: 29142117

Start: 1747485

End: 1748150

Strand: Direct

Name: rluB

Synonym: t1683

Alternate gene names: 29142117

Gene position: 1747485-1748150 (Clockwise)

Preceding gene: 29142114

Following gene: 29142118

Centisome position: 36.47

GC content: 52.85

Gene sequence:

>666_bases
ATGCGGCAATTAATAAGTTCTGAAAACACCATGCAAAAAACTTCTTTTAGAAATCACTACGTTAAGCGATTCAGCTCACG
ACAAGCATCTAAATCACGGAAAGAAAACCAGCCAAAACGCGTGGTTTTGTTCAATAAACCTTACGATGTGTTGCCGCAGT
TCACGGATGAAGCAGGTCGCCGCACGTTAAAAGATTTTATTCCTGTGCAAGGCGTTTATGCCGCAGGCCGCCTGGACCGC
GACAGCGAAGGGCTTTTGGTGCTGACTAATGACGGCGCGCTGCAGGCGCGCCTCACGCAACCGGGCAAACGCACCGGCAA
GATTTACTATGTTCAGGTAGAAGGCATCCCGGATAATGCAGCGCTGCAGGCGCTTCGTACTGGCGTCACGCTCAACGATG
GCCCTACCCTGCCCGCCGGTATTGAGATTGTCGCGGAGCCTGACTGGCTGTGGCCGCGCACCCCGCCGATTCGCGAACGG
AAGAATATTCCCACCAGTTGGCTAAAAGTCACCTTATATGAAGGCCGCAACCACCAGGTAAGACGCATGACAGCGCATGT
CGGTCATCCCACTCTACGATTAATTCGTTACAGCATGGGCGACTACACGCTCAACGGCCTCGATAATGGACAGTGGTGCG
AGATCGCACAGGAGAAAGACAGATGA

Upstream 100 bases:

>100_bases
ACGACTGCGTTAGAGCAATTTTTTGTTAATGTTTTGTAATGAGCATGTCAATACTACCCTATTGTTTGCCGCCATGAAAG
ACACTCATTATTGCGCTATA

Downstream 100 bases:

>100_bases
AACGGCTTCTCATTGCTTTTGCTTTATTAACACCGTTATCCGTTAATGCGGCCAGCTTCGACTGCCAGAAAGCGCAGGCT
GCGGATGAAAAGGCTATCTG

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTDEAGRRTLKDFIPVQGVYAAGRLDR
DSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNAALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPPIRER
KNIPTSWLKVTLYEGRNHQVRRMTAHVGHPTLRLIRYSMGDYTLNGLDNGQWCEIAQEKDR

Sequences:

>Translated_221_residues
MRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTDEAGRRTLKDFIPVQGVYAAGRLDR
DSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNAALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPPIRER
KNIPTSWLKVTLYEGRNHQVRRMTAHVGHPTLRLIRYSMGDYTLNGLDNGQWCEIAQEKDR
>Mature_221_residues
MRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTDEAGRRTLKDFIPVQGVYAAGRLDR
DSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNAALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPPIRER
KNIPTSWLKVTLYEGRNHQVRRMTAHVGHPTLRLIRYSMGDYTLNGLDNGQWCEIAQEKDR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family

Homologues:

Organism=Escherichia coli, GI87081838, Length=215, Percent_Identity=82.3255813953488, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787524, Length=183, Percent_Identity=37.1584699453552, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1788510, Length=187, Percent_Identity=32.620320855615, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790453, Length=187, Percent_Identity=26.2032085561497, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUE_SALTI (Q8Z7G6)

Other databases:

- EMBL:   AL627269
- EMBL:   AE014613
- RefSeq:   NP_455729.1
- RefSeq:   NP_805459.1
- ProteinModelPortal:   Q8Z7G6
- SMR:   Q8Z7G6
- GeneID:   1071300
- GeneID:   1247680
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t1683
- KEGG:   sty:STY1277
- HOGENOM:   HBG754478
- OMA:   CQFSPHE
- ProtClustDB:   CLSK894081
- BioCyc:   SENT209261:T1683-MONOMER
- BioCyc:   SENT220341:STY1277-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 25258; Mature: 25258

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS01149 PSI_RSU

Important sites: ACT_SITE 79-79

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTDEAGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCHHHHHHH
RTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNA
HHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCCHH
ALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPPIRERKNIPTSWLKVTLYEGRNHQV
HHHHHHHCCEECCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCEE
RRMTAHVGHPTLRLIRYSMGDYTLNGLDNGQWCEIAQEKDR
EEEEHHCCCCCEEHEEECCCCEEECCCCCCCEEECCCCCCC
>Mature Secondary Structure
MRQLISSENTMQKTSFRNHYVKRFSSRQASKSRKENQPKRVVLFNKPYDVLPQFTDEAGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCHHHHHHH
RTLKDFIPVQGVYAAGRLDRDSEGLLVLTNDGALQARLTQPGKRTGKIYYVQVEGIPDNA
HHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCCHH
ALQALRTGVTLNDGPTLPAGIEIVAEPDWLWPRTPPIRERKNIPTSWLKVTLYEGRNHQV
HHHHHHHCCEECCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCEE
RRMTAHVGHPTLRLIRYSMGDYTLNGLDNGQWCEIAQEKDR
EEEEHHCCCCCEEHEEECCCCEEECCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504