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The map label for this gene is sapA [H]

Identifier: 29142037

GI number: 29142037

Start: 1655071

End: 1656720

Strand: Direct

Name: sapA [H]

Synonym: t1597

Alternate gene names: 29142037

Gene position: 1655071-1656720 (Clockwise)

Preceding gene: 29142036

Following gene: 29142038

Centisome position: 34.54

GC content: 53.82

Gene sequence:

>1650_bases
ATGCGCCTGGTTTTATCATCTCTGATCGTGATAGCGGGTCTACTGAGTAGTCAGGCTACGGCTGCGACTGCGCCCGAACA
AACTGCGAGTGCAGATATTCGCGATAGCGGCTTTGTGTATTGTGTCAGCGGGCAGGTCAACACCTTTAATCCGCAAAAAG
CGAGTAGCGGCCTCATCGTCGATACCCTGGCCGCCCAGTTATATGACCGCCTGTTGGATGTCGATCCCTATACTTATCGT
TTAGTCCCAGAGCTGGCAGAAAGCTGGGAAGTGCTGGATAACGGGGCAACGTACCATTTTCACCTGCGCCGCGACGTTTC
CTTTCAAAAAACCGCCTGGTTTACGCCGACCCGAAAACTCAATGCTGATGATGTCGTCTTTACCTTTCAGCGGATTTTCG
ATCGTCGACATCCGTGGCATAACATCAACGGCAGTAGCTTCCCCTACTTTGATAGCCTACAGTTCGCCGACAATGTAAAA
AGCGTGCGTAAGCTGGACAATAACACCGTTGAGTTTCGCCTGACGCAGCCAGACGCCTCCTTTTTATGGCATCTGGCCAC
GCACTACGCTTCCGTCATGTCTGCTGAGTACGCCGCGCAGCTTAGCCGAAAAGATCGTCAGGAACTGCTTGATCGGCAAC
CGGTCGGCACCGGGCCTTTCCAGCTTTCGGAGTACCGTGCCGGGCAGTTTATTCGTCTCCAGCGCCACGATGGGTTTTGG
CGCGGCAAACCGCTAATGCCGCAAGTGGTGGTGGATTTAGGCTCCGGGGGTATTGGGCGTTTATCGAAATTACTGACCGG
TGAATGCGATGTTCTGGCCTGGCCCGCCGCCAGCCAGCTAACTATTTTACGCGACGATCCGCGTTTGCGTCTGACGTTGC
GCCCGGGGATGAATATCGCCTATCTGGCCTTTAACACCGACAAGCCGCCGTTGAATAATCCCGCAGTGCGCCATGCGCTG
GCCTTATCGATCAACAACCAGCGCCTGATGCAGTCGATTTATTACGGCACGGCGGAAACCGCAGCCTCCATTTTACCGAG
AGCCTCATGGGCTTACGATAACGATGCCAAAATTACGGAGTACAATCCGCAAAAATCGCGCGAACAACTAAAAGCGCTGG
GCATTGAGAATCTTACGCTGCATCTCTGGGTGCCGACCAGTTCTCAGGCCTGGAACCCAAGCCCGCTAAAAACGGCGGAG
CTTATTCAGGCGGATATGGCGCAAGTTGGCGTAAAAGTGGTCATTGTGCCGGTTGAAGGTCGTTTTCAGGAGGCGCGCCT
GATGGATATGAATCACGATCTGACCTTATCCGGCTGGGCCACGGACAGCAACGATCCGGATAGCTTTTTCAGACCGCTGT
TAAGCTGTGCGGCCATCAATTCGCAAACCAATTTCGCCCACTGGTGTAACCCTGAATTTGACAGCGTGCTGCGTAAGGCA
CTGTCGTCGCAGCAGTTGGCTTCGCGCATAGAAGCGTATGACGAAGCGCAGAATATCCTGGAGAAAGAGCTGCCGATACT
GCCGCTGGCATCATCACTACGCTTACAGGCTTACCGCTACGATATTAAAGGACTGGTGTTAAGCCCGTTCGGCAATGCGT
CTTTTGCCGGCGTCTCCCGCGAAAAACACGAAGAGGTGAAAAAACCATGA

Upstream 100 bases:

>100_bases
GCTAATTGACGACATTTACGCCAGTTATCCACCGACATTTTTACGTGGCGGGCCGAAGTGCGATACACTTTGCAAATTGA
ACTTCAAAAACTTAACTATT

Downstream 100 bases:

>100_bases
TTATCTTCACCCTGCGTCGGTTATTGCTGTTGCTGGTGACGCTATTCTTCCTGACCTTTATCGGCTTTAGCCTGAGCTAT
TTTACGCCGCACGCGCCGCT

Product: peptide transport periplasmic protein SapA

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MRLVLSSLIVIAGLLSSQATAATAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYR
LVPELAESWEVLDNGATYHFHLRRDVSFQKTAWFTPTRKLNADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVK
SVRKLDNNTVEFRLTQPDASFLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
RGKPLMPQVVVDLGSGGIGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHAL
ALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITEYNPQKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAE
LIQADMAQVGVKVVIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
LSSQQLASRIEAYDEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSREKHEEVKKP

Sequences:

>Translated_549_residues
MRLVLSSLIVIAGLLSSQATAATAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYR
LVPELAESWEVLDNGATYHFHLRRDVSFQKTAWFTPTRKLNADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVK
SVRKLDNNTVEFRLTQPDASFLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
RGKPLMPQVVVDLGSGGIGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHAL
ALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITEYNPQKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAE
LIQADMAQVGVKVVIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
LSSQQLASRIEAYDEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSREKHEEVKKP
>Mature_549_residues
MRLVLSSLIVIAGLLSSQATAATAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYR
LVPELAESWEVLDNGATYHFHLRRDVSFQKTAWFTPTRKLNADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVK
SVRKLDNNTVEFRLTQPDASFLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
RGKPLMPQVVVDLGSGGIGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHAL
ALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITEYNPQKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAE
LIQADMAQVGVKVVIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
LSSQQLASRIEAYDEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSREKHEEVKKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=549, Percent_Identity=89.9817850637523, Blast_Score=1002, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=515, Percent_Identity=36.504854368932, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787052, Length=547, Percent_Identity=27.0566727605119, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1787762, Length=490, Percent_Identity=23.8775510204082, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1787495, Length=549, Percent_Identity=24.2258652094718, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=23.5887096774194, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789397, Length=534, Percent_Identity=23.9700374531835, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87081878, Length=534, Percent_Identity=24.5318352059925, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61630; Mature: 61630

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVLSSLIVIAGLLSSQATAATAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIV
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
DTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYHFHLRRDVSFQKTAWFTPTRKL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
NADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVKSVRKLDNNTVEFRLTQPDAS
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
FLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEECCCCCC
RGKPLMPQVVVDLGSGGIGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIA
CCCCCCHHHHEECCCCCHHHHHHHHCCCCCEEECCCCCCEEEEECCCEEEEEECCCCCEE
YLAFNTDKPPLNNPAVRHALALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITE
EEEEECCCCCCCCHHHHEEEEEEECHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEE
YNPQKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCC
RFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
CCCHHHEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LSSQQLASRIEAYDEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCCCCCH
EKHEEVKKP
HHHHHHCCC
>Mature Secondary Structure
MRLVLSSLIVIAGLLSSQATAATAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIV
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
DTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYHFHLRRDVSFQKTAWFTPTRKL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
NADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVKSVRKLDNNTVEFRLTQPDAS
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
FLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEECCCCCC
RGKPLMPQVVVDLGSGGIGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIA
CCCCCCHHHHEECCCCCHHHHHHHHCCCCCEEECCCCCCEEEEECCCEEEEEECCCCCEE
YLAFNTDKPPLNNPAVRHALALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITE
EEEEECCCCCCCCHHHHEEEEEEECHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEE
YNPQKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCC
RFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
CCCHHHEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LSSQQLASRIEAYDEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCCCCCH
EKHEEVKKP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]