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The map label for this gene is ogt

Identifier: 29142003

GI number: 29142003

Start: 1624332

End: 1624847

Strand: Direct

Name: ogt

Synonym: t1563

Alternate gene names: 29142003

Gene position: 1624332-1624847 (Clockwise)

Preceding gene: 29141999

Following gene: 161486799

Centisome position: 33.9

GC content: 55.23

Gene sequence:

>516_bases
ATGCTGAGATTACTTGAAGAGAAGATAGCCACGCCATTAGGTCCGTTATGGGTGGTTTGCGATGAGCAGTTTCGACTGCG
GGCCATTGAGTGGGAACAGTACCGCGATCGTATGGAGCAACTGCTAAATATCCACTACCGTCACGAAGGCTATGAACGCG
TTTCTGCGACTAACCCCGGTGGACTCAGCGATAAGCTTGCAGATTATTTTGCAGGCAATCTCGCCGTAATTGATACCTTG
GAAACCGCCACGGGGGGCACACCTTTTCAACGGGAAGTATGGCAGGCGTTGCGCGCTATCCCCTGCGGGCAAGTGATGCA
CTATGGCCAACTGGCGGCGCAACTGGGACGACCGGGCGCCGCACGCGCAGTGGGTGCTGCGAATGGCGCTAACCCCATCA
GTATTGTTGTTCCCTGCCATCGCGTCATCGGGCGTAACGGCACTCTGACCGGATACGCAGGCGGCGTGCAGCGAAAAGAG
TGGCTATTACGCCATGAAGGCTATCTTTTATTATGA

Upstream 100 bases:

>100_bases
AATGTGTTATCCCTGACTATCTTTTAAGGAGTATGGTTGCGGGTATTCCTGGCATGATATCTTGTCTCTTACGTTAGATT
AAGACGATGTGAGAGAACGG

Downstream 100 bases:

>100_bases
ATATACAGGCAAAAAGTGCCTTATCGGTCACACTTTTATGTAAAGTAGCAACAAATAAATTACGTATTTTCAAATAGATA
TAAGTATTCATCAAACTTAT

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERVSATNPGGLSDKLADYFAGNLAVIDTL
ETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGAARAVGAANGANPISIVVPCHRVIGRNGTLTGYAGGVQRKE
WLLRHEGYLLL

Sequences:

>Translated_171_residues
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERVSATNPGGLSDKLADYFAGNLAVIDTL
ETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGAARAVGAANGANPISIVVPCHRVIGRNGTLTGYAGGVQRKE
WLLRHEGYLLL
>Mature_171_residues
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERVSATNPGGLSDKLADYFAGNLAVIDTL
ETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGAARAVGAANGANPISIVVPCHRVIGRNGTLTGYAGGVQRKE
WLLRHEGYLLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=83, Percent_Identity=50.6024096385542, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=171, Percent_Identity=88.3040935672515, Blast_Score=322, Evalue=7e-90,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=53.9325842696629, Blast_Score=103, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=48.1481481481481, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_SALTI (P0A2U1)

Other databases:

- EMBL:   AL627270
- EMBL:   AE014613
- RefSeq:   NP_455845.1
- RefSeq:   NP_805345.1
- ProteinModelPortal:   P0A2U1
- SMR:   P0A2U1
- PRIDE:   P0A2U1
- GeneID:   1071042
- GeneID:   1247800
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t1563
- KEGG:   sty:STY1405
- HOGENOM:   HBG752398
- OMA:   NLRAVEW
- ProtClustDB:   PRK10286
- BioCyc:   SENT209261:T1563-MONOMER
- BioCyc:   SENT220341:STY1405-MONOMER
- BRENDA:   2.1.1.63
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 19008; Mature: 19008

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERVSATNPG
CCCHHHHHHCCCCCCEEEEECCCHHEEEECHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
GLSDKLADYFAGNLAVIDTLETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGA
CCHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGANPISIVVPCHRVIGRNGTLTGYAGGVQRKEWLLRHEGYLLL
HHECCCCCCCCCEEEEEEHHHHHCCCCCEECCCCCCHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERVSATNPG
CCCHHHHHHCCCCCCEEEEECCCHHEEEECHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
GLSDKLADYFAGNLAVIDTLETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGA
CCHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGANPISIVVPCHRVIGRNGTLTGYAGGVQRKEWLLRHEGYLLL
HHECCCCCCCCCEEEEEEHHHHHCCCCCEECCCCCCHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504