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The map label for this gene is ldhA [H]

Identifier: 29141991

GI number: 29141991

Start: 1607545

End: 1608534

Strand: Direct

Name: ldhA [H]

Synonym: t1550

Alternate gene names: 29141991

Gene position: 1607545-1608534 (Clockwise)

Preceding gene: 29141987

Following gene: 29141992

Centisome position: 33.55

GC content: 51.01

Gene sequence:

>990_bases
ATGAAACTCGCCGTCTATAGTACCAAACAGTATGACAAGAAATATCTACAGCAAGTGAACGAAGCGTTCGGTTTTGAACT
GGAGTTTTTTGATTTTCTGCTAACCGAGAAAACGGCGAAAACCGCCAATGGATGTGAAGCAGTTTGTATCTTCGTGAATG
ACGACGGGAGCCGACCGGTACTGGAAGAGCTGAAAAAACACGGCGTGAAATACATCGCGCTGCGTTGCGCCGGATTTAAT
AATGTCGACCTGGATGCCGCTAAAGAACTCGGCTTGCAGGTGGTGCGCGTACCGGCTTATTCTCCGGAAGCCGTCGCTGA
ACATGCGATTGGCATGATGATGACATTGAACCGCCGTATTCACCGCGCTTATCAACGCACCCGCGACGCTAACTTTTCCC
TTGAGGGACTGACCGGCTTTACCATGCACGGAAAAACCGCTGGCGTCATTGGTACCGGTAAAATCGGCGTCGCCGCGCTG
CGTATCCTGAAAGGCTTTGGAATGCGTTTACTGGCGTTTGATCCCTATCCCAGCACTGCCGCGCTTGACCTCGGCGTCGA
GTATGTGGATCTGCAAACGTTGTTTGCCGAATCGGATGTCATTTCCCTGCACTGCCCGCTCACGCCGGAAAACTACCATT
TACTGAACCATGCGGCATTCGATCAAATGAAAAATGGCGTTATGATCATTAATACCAGTCGTGGCGCGCTGATTGATTCC
CAGGCGGCTATCGAAGCTCTGAAGAATCAGAAAATCGGCTCGCTGGGGATGGATGTGTATGAAAACGAACGCGACCTGTT
CTTTGAGGACAAGTCAAACGATGTTATTCAGGATGATGTTTTCCGCCGTCTGTCGGCCTGCCATAACGTACTGTTTACGG
GGCATCAGGCATTCCTGACCGCAGAAGCGCTGATCAGCATCTCTGAAACCACGTTACAAAACTTAAGCCAGCTTGAGAAG
GGCGAAGCCTGCCCGAACGCGCTGTTCTAA

Upstream 100 bases:

>100_bases
AAAATTATTCATCATATGTATGACTATCAATTATTTTTGATTTTTTGTAAGATAATTTTAAGCATATTTGTGTGATTCTA
ACATCACTGGAGAAGGTCAT

Downstream 100 bases:

>100_bases
TCCTGTCGCTCCCCTGCGCTCAGAGGAGCATTTTCGGATAACCCCTGAAGATCTATCCGGTCATATCATTACTATAGTCA
GTGTTAACTATCAGATTACA

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKLAVYSTKQYDKKYLQQVNEAFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLQVVRVPAYSPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMHGKTAGVIGTGKIGVAAL
RILKGFGMRLLAFDPYPSTAALDLGVEYVDLQTLFAESDVISLHCPLTPENYHLLNHAAFDQMKNGVMIINTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALISISETTLQNLSQLEK
GEACPNALF

Sequences:

>Translated_329_residues
MKLAVYSTKQYDKKYLQQVNEAFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLQVVRVPAYSPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMHGKTAGVIGTGKIGVAAL
RILKGFGMRLLAFDPYPSTAALDLGVEYVDLQTLFAESDVISLHCPLTPENYHLLNHAAFDQMKNGVMIINTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALISISETTLQNLSQLEK
GEACPNALF
>Mature_329_residues
MKLAVYSTKQYDKKYLQQVNEAFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLQVVRVPAYSPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMHGKTAGVIGTGKIGVAAL
RILKGFGMRLLAFDPYPSTAALDLGVEYVDLQTLFAESDVISLHCPLTPENYHLLNHAAFDQMKNGVMIINTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALISISETTLQNLSQLEK
GEACPNALF

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=277, Percent_Identity=31.7689530685921, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4557497, Length=277, Percent_Identity=31.7689530685921, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI23308577, Length=200, Percent_Identity=33.5, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI145580578, Length=258, Percent_Identity=32.1705426356589, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4557499, Length=258, Percent_Identity=32.1705426356589, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI145580575, Length=254, Percent_Identity=32.6771653543307, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI6912396, Length=201, Percent_Identity=31.8407960199005, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=94.8170731707317, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=216, Percent_Identity=30.5555555555556, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25147481, Length=277, Percent_Identity=28.5198555956679, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17532191, Length=254, Percent_Identity=24.8031496062992, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=193, Percent_Identity=33.160621761658, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=29.5138888888889, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=31.4655172413793, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=32.9457364341085, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=32.9457364341085, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=32.9457364341085, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=32.9457364341085, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=32.9457364341085, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=26.9709543568465, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574286, Length=261, Percent_Identity=26.8199233716475, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45552429, Length=201, Percent_Identity=29.3532338308458, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585514, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574282, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574284, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551003, Length=201, Percent_Identity=29.3532338308458, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571528, Length=208, Percent_Identity=28.3653846153846, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36431; Mature: 36431

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVYSTKQYDKKYLQQVNEAFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLQVVRVPAYSPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMHGKTAGVIGTGKIGVAALRILKGFGMRLLAFDPYPSTA
HHHHHHHCCCCCEECCCEEEEECCCCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCCE
ALDLGVEYVDLQTLFAESDVISLHCPLTPENYHLLNHAAFDQMKNGVMIINTSRGALIDS
EEECCCEEEEHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALISISETTLQNLSQLEKGEACPNALF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLAVYSTKQYDKKYLQQVNEAFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLQVVRVPAYSPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMHGKTAGVIGTGKIGVAALRILKGFGMRLLAFDPYPSTA
HHHHHHHCCCCCEECCCEEEEECCCCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCCE
ALDLGVEYVDLQTLFAESDVISLHCPLTPENYHLLNHAAFDQMKNGVMIINTSRGALIDS
EEECCCEEEEHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALISISETTLQNLSQLEKGEACPNALF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]