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The map label for this gene is ydhE [H]

Identifier: 29141752

GI number: 29141752

Start: 1356368

End: 1357741

Strand: Reverse

Name: ydhE [H]

Synonym: t1293

Alternate gene names: 29141752

Gene position: 1357741-1356368 (Counterclockwise)

Preceding gene: 29141754

Following gene: 29141721

Centisome position: 28.33

GC content: 53.71

Gene sequence:

>1374_bases
GTGCAGAAGTATACCAGTGAAGCGCGTCAGTTATTAGCGTTAGCGATACCGGTGATCCTTGCGCAAGTGGCACAGACCGC
AATGGGATTCGTCGATACCGTCATGGCGGGAGGCTATAGCGCTACGGACATGGCTGCCGTCGCTATCGGCACCTCTATCT
GGCTTCCCGCCATTTTGTTTGGTCACGGCTTACTGCTGGCCTTGACGCCTGTGATTGCGCAACTCAATGGCTCCGGCCGT
CGTGAGCGTATCGCGCATCAGGTTCGACAAGGGTTCTGGCTGGCGGGCTTTGTCTCCGTGCTGGTGATGATCGTCCTGTG
GAATGCCGGATACATTATCCGCTCTATGCATAATATTGATCCGGCGCTGGCCGATAAAGCCGTGGGTTATCTTCGCGCGC
TGCTGTGGGGCGCGCCAGGATACCTTTTCTTTCAGGTAGCGCGAAACCAGTGCGAAGGTCTGGCCAAAACCAAACCGGGT
ATGGTGATGGGATTTTTGGGCCTGCTGGTGAATATTCCGGTGAACTATATTTTTATCTATGGCCATTTTGGTATGCCGGA
ACTCGGCGGTATCGGCTGCGGCGTTGCGACTGCGGCGGTCTATTGGGTGATGTTTATCGCCATGCTTTCTTACATTAAAC
ACGCGCGTTCAATGCGTGATATTCGTAATGAAAAAGGCTTTGGCAAACCTGATAGCGTCGTCATGAAGCGCCTGATTCAA
CTGGGGTTGCCGATTGCGCTGGCGCTGTTCTTTGAAGTGACGTTGTTTGCGGTCGTTGCGCTGCTGGTTTCGCCATTAGG
TATTGTGGATGTCGCCGGTCATCAGATTGCGCTTAACTTTAGCTCGCTGATGTTCGTACTGCCGATGTCGCTGGCTGCGG
CGGTAACGATTCGAGTGGGTTATCGTCTGGGCCAGGGCTCAACGCTGGATGCGCAAACCGCAGCGCGTACCGGTTTAGGC
GTCGGCATTTGTATGGCTGTGGTTACCGCTATTTTTACCGTTACTCTGCGTAAGCATATTGCTTTGCTCTACAATGACAA
CCCTGAGGTCGTGGCGCTTGCCGCGCAATTAATGCTGCTCGCTGCGGTTTACCAGATTTCCGACTCTATACAGGTTATCG
GCAGCGGTATTCTGCGCGGTTATAAAGATACGCGTTCCATCTTTTTTATCACCTTTACCGCCTACTGGGTGTTAGGTTTA
CCCAGCGGTTATATCCTGGCGTTAACGGATTTGGTGGTGGATCGTATGGGACCGGCAGGCTTCTGGATGGGGTTCATCAT
CGGCCTGACCTCGGCGGCGGTGTTAATGATGCTACGGATGCGCTATCTACAACGTCAGCCGTCCGCCATCATTTTGCAGC
GCGCTGCGCGATAA

Upstream 100 bases:

>100_bases
CCCCGCTACAATAGACTGAAATTTCCCCTGCTATTTCTTTTGGCTGCCACTACGGCGGCTTTTTAGTCTTTCATATAACA
ACAAAATAAAAGGTGAATAC

Downstream 100 bases:

>100_bases
TACGACGATAGCCGTCGCATGGCGGCTATTTTTTCGACATGTTGCATATTTCCACCGCAATCAGGTGATTTCAGGAAGAA
AATTGCGATTTCCCTCTTGC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFLGLLVNIPVNYIFIYGHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNEKGFGKPDSVVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLG
VGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVDRMGPAGFWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR

Sequences:

>Translated_457_residues
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFLGLLVNIPVNYIFIYGHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNEKGFGKPDSVVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLG
VGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVDRMGPAGFWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR
>Mature_457_residues
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFLGLLVNIPVNYIFIYGHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNEKGFGKPDSVVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLG
VGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVDRMGPAGFWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=451, Percent_Identity=21.7294900221729, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI153792564, Length=426, Percent_Identity=21.1267605633803, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38261960, Length=462, Percent_Identity=20.7792207792208, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=92.7789934354486, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=459, Percent_Identity=24.400871459695, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320545, Length=441, Percent_Identity=21.7687074829932, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321821, Length=393, Percent_Identity=20.3562340966921, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49427; Mature: 49427

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFLGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNEKGFGKPDSVVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLGVGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLL
EEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVDRMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFLGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNEKGFGKPDSVVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLGVGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLL
EEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVDRMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA