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The map label for this gene is puuP [H]

Identifier: 29141710

GI number: 29141710

Start: 1316421

End: 1317764

Strand: Reverse

Name: puuP [H]

Synonym: t1247

Alternate gene names: 29141710

Gene position: 1317764-1316421 (Counterclockwise)

Preceding gene: 29141711

Following gene: 29141709

Centisome position: 27.5

GC content: 50.22

Gene sequence:

>1344_bases
ATGAAAAAAGTCAAAAAATTGTCTCTTACCGATTTAGTGCTTTATGGTCTGGTGTTTATGGTGCCTATCGCACCAGTCTC
TCTTTATGGCGTCGTTTATAATCTTTCGCACGGTATGGTTGCGCTGGTTTATATTATTGGCGCTATCGCGATGTTTTTTA
CCGCGTACAGCTACTCTACCTTATCGCAGCATATCTCCTCGTCGGGTTCGGCCTATGCGTATGCTGGCGTATGCATTAAT
CCCGCCGTAGGGTTTCTGACCGGATGGATATTACTGCTGGATTATCTTTTGTTCCCTACGCTCGTAGCGGTGCTGGGCGG
TGTCGCGGTCCACGCTATTTTACCGCAAATCCCGGTCTGGGTCTGGCCGTTGATTTATGTGGCTATTGGCACCGGAGTCA
ACTATCTCGGGATACAGCAAACGGCGAAATTCGATAAGTTACTGGTCTTTATTCAACTCGGTATTCTGGCGATTTTCGTG
CTACTCATTATCCGTCTGATGATGCAGGACAGTAGTCAGATAACGCTTTCCTTCCGGCCTTTTTTCGATTCACAGTGGTT
CACCCCTGGGCTCATCGCAACGGCGATTTCGGTGGCTGCGCTGAATTTTCTCGGCTTTGACGCTATCAGTACATTAAGTG
AAGAAAGCGAAGGCGGGGGACGCGCCGTCAGTAAAGCAACACTGTTGGCGTTAGTTTTGGCGACGGTACTGTTTATTATC
GTTGTGGCGTTTGCGGCGTTCGCCACCGGTAACGTCGACCGCTTCGCGGAAGGTAATGCGACGAATGAAGCATTTTTCAC
CATTGCCGGCAACGTTGGCGGTATCTGGCTCAAAGTGGCGTTTTCCATCATTGTGGCGTTTGTGTGCGCCGTCGGCAACA
TTATTACCGCGCAAACGGCGGTGTCCAGAGTGTTATTTTCAATGGGGCGCGACCGAATGTTGCCCGCCTTCCTCGCTCAC
GTCCATACCACGCGTAAAACGCCGGATTATGCCATTCTGTTTACCGGCGGCGTCACCCTGCTGCTCAGCTACCTGTTTTC
CGGCAAGATTGAATCTATCTCCACGCTGGTGAACTTTGGCGCGCTTTTCGCTTTTTTTGTCGTGAACCTGTGTGTGTTTA
TCCTGTTCAATTTCCGGATGAAAGCCCAGCGGCGCATTTTCGCCCATGTCATTTCGCCGATCATGGGGATGATCGTCATC
GGCTACGTCTGTTTAAACATGAACATTCACGCTCTGATACTCGGAATTAGCTGGGCGGCGATCGGGATAGCGATACTGTG
CTACCGAAAAGCATACAACCAAAACATCGCCATCGACCTGGAAGGCAAAAAGTTGCTCGATTGA

Upstream 100 bases:

>100_bases
CAGCAGCCATAACCCGCATGAAGAAAATATCGCGCCCTGCCTCGCAGAGGTAAGCGCGTTTTTACGCGATCTGGCATAAA
AAGCCATTAAGATATTTATC

Downstream 100 bases:

>100_bases
CATTATGCGCAACAACACCAGCTGTTGCGCAACGCCGCCCCTGTTCGGGCGGCGTCATCGCCAGCGACATCGTAAACAGC
GTAATAACAAACCGTTGTGG

Product: amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKVKKLSLTDLVLYGLVFMVPIAPVSLYGVVYNLSHGMVALVYIIGAIAMFFTAYSYSTLSQHISSSGSAYAYAGVCIN
PAVGFLTGWILLLDYLLFPTLVAVLGGVAVHAILPQIPVWVWPLIYVAIGTGVNYLGIQQTAKFDKLLVFIQLGILAIFV
LLIIRLMMQDSSQITLSFRPFFDSQWFTPGLIATAISVAALNFLGFDAISTLSEESEGGGRAVSKATLLALVLATVLFII
VVAFAAFATGNVDRFAEGNATNEAFFTIAGNVGGIWLKVAFSIIVAFVCAVGNIITAQTAVSRVLFSMGRDRMLPAFLAH
VHTTRKTPDYAILFTGGVTLLLSYLFSGKIESISTLVNFGALFAFFVVNLCVFILFNFRMKAQRRIFAHVISPIMGMIVI
GYVCLNMNIHALILGISWAAIGIAILCYRKAYNQNIAIDLEGKKLLD

Sequences:

>Translated_447_residues
MKKVKKLSLTDLVLYGLVFMVPIAPVSLYGVVYNLSHGMVALVYIIGAIAMFFTAYSYSTLSQHISSSGSAYAYAGVCIN
PAVGFLTGWILLLDYLLFPTLVAVLGGVAVHAILPQIPVWVWPLIYVAIGTGVNYLGIQQTAKFDKLLVFIQLGILAIFV
LLIIRLMMQDSSQITLSFRPFFDSQWFTPGLIATAISVAALNFLGFDAISTLSEESEGGGRAVSKATLLALVLATVLFII
VVAFAAFATGNVDRFAEGNATNEAFFTIAGNVGGIWLKVAFSIIVAFVCAVGNIITAQTAVSRVLFSMGRDRMLPAFLAH
VHTTRKTPDYAILFTGGVTLLLSYLFSGKIESISTLVNFGALFAFFVVNLCVFILFNFRMKAQRRIFAHVISPIMGMIVI
GYVCLNMNIHALILGISWAAIGIAILCYRKAYNQNIAIDLEGKKLLD
>Mature_447_residues
MKKVKKLSLTDLVLYGLVFMVPIAPVSLYGVVYNLSHGMVALVYIIGAIAMFFTAYSYSTLSQHISSSGSAYAYAGVCIN
PAVGFLTGWILLLDYLLFPTLVAVLGGVAVHAILPQIPVWVWPLIYVAIGTGVNYLGIQQTAKFDKLLVFIQLGILAIFV
LLIIRLMMQDSSQITLSFRPFFDSQWFTPGLIATAISVAALNFLGFDAISTLSEESEGGGRAVSKATLLALVLATVLFII
VVAFAAFATGNVDRFAEGNATNEAFFTIAGNVGGIWLKVAFSIIVAFVCAVGNIITAQTAVSRVLFSMGRDRMLPAFLAH
VHTTRKTPDYAILFTGGVTLLLSYLFSGKIESISTLVNFGALFAFFVVNLCVFILFNFRMKAQRRIFAHVISPIMGMIVI
GYVCLNMNIHALILGISWAAIGIAILCYRKAYNQNIAIDLEGKKLLD

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI115648063, Length=371, Percent_Identity=25.0673854447439, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI115648022, Length=371, Percent_Identity=25.0673854447439, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI7657683, Length=367, Percent_Identity=23.7057220708447, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI186910308, Length=373, Percent_Identity=24.6648793565684, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI186910306, Length=373, Percent_Identity=24.6648793565684, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI186910304, Length=373, Percent_Identity=24.6648793565684, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4507047, Length=378, Percent_Identity=24.6031746031746, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081869, Length=430, Percent_Identity=34.1860465116279, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082023, Length=439, Percent_Identity=30.9794988610478, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081708, Length=414, Percent_Identity=22.9468599033816, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32566699, Length=347, Percent_Identity=23.9193083573487, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532491, Length=415, Percent_Identity=24.578313253012, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17533459, Length=401, Percent_Identity=23.4413965087282, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221512776, Length=414, Percent_Identity=23.4299516908213, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24666159, Length=414, Percent_Identity=23.4299516908213, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24667468, Length=418, Percent_Identity=23.9234449760766, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21357367, Length=372, Percent_Identity=22.5806451612903, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 48472; Mature: 48472

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKKLSLTDLVLYGLVFMVPIAPVSLYGVVYNLSHGMVALVYIIGAIAMFFTAYSYST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSQHISSSGSAYAYAGVCINPAVGFLTGWILLLDYLLFPTLVAVLGGVAVHAILPQIPVW
HHHHHHCCCCEEEEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VWPLIYVAIGTGVNYLGIQQTAKFDKLLVFIQLGILAIFVLLIIRLMMQDSSQITLSFRP
HHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
FFDSQWFTPGLIATAISVAALNFLGFDAISTLSEESEGGGRAVSKATLLALVLATVLFII
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VVAFAAFATGNVDRFAEGNATNEAFFTIAGNVGGIWLKVAFSIIVAFVCAVGNIITAQTA
HHHHHHHHCCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
VSRVLFSMGRDRMLPAFLAHVHTTRKTPDYAILFTGGVTLLLSYLFSGKIESISTLVNFG
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHH
ALFAFFVVNLCVFILFNFRMKAQRRIFAHVISPIMGMIVIGYVCLNMNIHALILGISWAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IGIAILCYRKAYNQNIAIDLEGKKLLD
HHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKKVKKLSLTDLVLYGLVFMVPIAPVSLYGVVYNLSHGMVALVYIIGAIAMFFTAYSYST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSQHISSSGSAYAYAGVCINPAVGFLTGWILLLDYLLFPTLVAVLGGVAVHAILPQIPVW
HHHHHHCCCCEEEEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VWPLIYVAIGTGVNYLGIQQTAKFDKLLVFIQLGILAIFVLLIIRLMMQDSSQITLSFRP
HHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
FFDSQWFTPGLIATAISVAALNFLGFDAISTLSEESEGGGRAVSKATLLALVLATVLFII
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VVAFAAFATGNVDRFAEGNATNEAFFTIAGNVGGIWLKVAFSIIVAFVCAVGNIITAQTA
HHHHHHHHCCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
VSRVLFSMGRDRMLPAFLAHVHTTRKTPDYAILFTGGVTLLLSYLFSGKIESISTLVNFG
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHH
ALFAFFVVNLCVFILFNFRMKAQRRIFAHVISPIMGMIVIGYVCLNMNIHALILGISWAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IGIAILCYRKAYNQNIAIDLEGKKLLD
HHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]