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The map label for this gene is celF [H]

Identifier: 29141660

GI number: 29141660

Start: 1262820

End: 1264175

Strand: Direct

Name: celF [H]

Synonym: t1195

Alternate gene names: 29141660

Gene position: 1262820-1264175 (Clockwise)

Preceding gene: 29141659

Following gene: 29141661

Centisome position: 26.35

GC content: 47.94

Gene sequence:

>1356_bases
ATGAGCCAGAAATTAAAAGTCGTCACTATTGGTGGCGGGAGCAGCTACACCCCTGAATTACTTGAAGGCTTTATTAAGCG
CTACCATGAATTACCTGTCACCGAATTATGGCTGGTTGACGTTGAAGACGGGAAAAAGAAGCTGGGCATTATTTATGATC
TCTGCCAGCGAATGATTGATAAAGCAGGCGTTCCGCTAAAATTGTATAAAACGCTGGATCGCCGGGAAGCGCTGAAAGGC
GCTAATTTTGTCACTACCCAGCTACGCGTTGGTCAACTCAAAGCCCGTGAGCTGGACGAGCGTATCCCGCTTAGCCACGG
CTATCTGGGGCAAGAAACCAACGGCGCTGGCGGTTTATTTAAAGGGTTGCGTACCATTCCGGTTATTTTTGACATCATTA
AGGATGTTGAAGAATTATGTCCGAATGCGTGGGTCATTAACTTTACTAACCCGGCGGGGATGGTGACGGAAGCGGTTTAT
CGTCATACCAACTTTAAAAAATTCATCGGCGTATGTAATATTCCTGTCGACATGAAAATGTTTATTCATGACGTGCTGGC
GCTGAATGAGAATGACGATCTTTCCATTGACCTGTTTGGTCTAAACCATATGGTCTTTATTAAAGATGTGCTGGTGAATG
GCACCTCACGGTTCGCAGAATTACTGGATGGCGTGGCGTCCGGTCAGTTGAAAGCGTCAACCGTAAAAAATATCTTTGAT
CTGCCGTTTAGTGAAGGATTGATTCGCTCGCTGAACATGCTGCCGTGCTCTTATTTGTTGTATTACTTCAAGCAAAAAGA
GATGCTGGCGATTGAAATGGGCGAATATTACAAAGGCGGCGCGCGCGCTCAGGTCGTACAAAAAGTGGAGAAACAACTCT
TCGACTTGTACAAAAATCCTGAGCTAAACGTGAAGCCGAAAGAGCTTGAGCAACGCGGCGGCGCTTATTATTCCGATGCC
GCTTGTGAAGTCATTAACGCTATTTATAATGACAAGCAGACTGAGCATTATGTTAATATTCCACATCATGGGCATGTCGA
GAATATCCCGGCGGACTGGGCGGTGGAAATGACCTGCATTCTGGGACGCAATGGCGCGACGCCGCACCCGCGTATCACCC
GTTTTGACGAAAAAGTGCTGGGGCTTATCCACACTATTAAAGGATTTGAGGTCGCGGCCAGCAATGCGGCGCTGAGCGGA
AACTTTAATGATGTGCTGCTGGCGCTTAACCTGAGTCCGCTGGTGCATTCCGACCGAGACGCAGAAGTCCTGGCGCGTGA
GCTCATTCTGGCGCATGAAAAATGGCTGCCTAATTTTGCCGCTTGCATCGAAGCGCTTAAAGGTAAGCACCACTGA

Upstream 100 bases:

>100_bases
CATTTCACGCTTGCCCCAATAGCGCGGGGAACGTCACGCTATTTCTTACGAGCGGGCATAGCGTAATACATATCAGTGTC
ATACACTGAAGGGAGATATT

Downstream 100 bases:

>100_bases
CAGAGGTAGGCTATGGAACGCGTGTTGATTGTGAATGCCGATGATTTTGGCCTGAGTAAAGGGCAAAATTACGGCATTGT
TGAAGCATATCGCAACGGCG

Product: phospho-beta-glucosidase B

Products: NA

Alternate protein names: Cellobiose-6-phosphate hydrolase; Phospho-chitobiase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKKKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKG
ANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVY
RHTNFKKFIGVCNIPVDMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDA
ACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSG
NFNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH

Sequences:

>Translated_451_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKKKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKG
ANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVY
RHTNFKKFIGVCNIPVDMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDA
ACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSG
NFNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH
>Mature_450_residues
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKKKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKGA
NFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYR
HTNFKKFIGVCNIPVDMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFDL
PFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDAA
CEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSGN
FNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH

Specific function: Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl- beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=450, Percent_Identity=90.2222222222222, Blast_Score=851, Evalue=0.0,
Organism=Escherichia coli, GI1790560, Length=468, Percent_Identity=25.4273504273504, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50596; Mature: 50465

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKKKLGIIYDLCQRMID
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
KAGVPLKLYKTLDRREALKGANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLF
HCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYRHTNFKKFIGVCNIPVDMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHEECCCCHHHHH
FIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHC
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCI
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEE
LGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSGNFNDVLLALNLSPLVHSDRD
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEEECCHHHCCCCC
AEVLARELILAHEKWLPNFAACIEALKGKHH
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKKKLGIIYDLCQRMID
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
KAGVPLKLYKTLDRREALKGANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLF
HCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYRHTNFKKFIGVCNIPVDMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHEECCCCHHHHH
FIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHC
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCI
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEE
LGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSGNFNDVLLALNLSPLVHSDRD
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEEECCHHHCCCCC
AEVLARELILAHEKWLPNFAACIEALKGKHH
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 8121401; 10572139; 9405618; 10913117 [H]