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The map label for this gene is nadE [H]

Identifier: 29141654

GI number: 29141654

Start: 1257996

End: 1258823

Strand: Reverse

Name: nadE [H]

Synonym: t1189

Alternate gene names: 29141654

Gene position: 1258823-1257996 (Counterclockwise)

Preceding gene: 29141663

Following gene: 29141653

Centisome position: 26.27

GC content: 53.02

Gene sequence:

>828_bases
ATGACTCTGCAGCAAGAGATAATCCAGGCGCTTGGCGCGAAACCGCATATCAACCCTGAAGAAGAAATTCGCCGCAGCGT
GGATTTTCTTAAAGCGTACCTGAAAACCTATCCCTTTTTGAAATCGCTGGTGTTAGGCATCAGCGGCGGGCAGGATTCGA
CGCTGGCCGGGAAACTCAGCCAGATGGCTATCACCGAATTGCGGGAAGAGACCGGTGATAATGCGCTGCAGTTTATCGCC
GTTCGTCTGCCTTATGGCGTGCAGGCCGATGAACAGGATTGCCAGGACGCCATTGCTTTTATTCAGCCGGACAGAGTACT
CACCGTCAATATTAAAGGCGCCGTCCTCGCCAGCGAACAGGCGCTGCGCGAAGCGGGAATTGAGCTGAGCGATTTTGTTC
GCGGCAATGAAAAAGCGCGTGAGCGAATGAAAGCGCAATATAGCATCGCCGGTATGACTCACGGCGTCGTGGTGGGCACC
GACCATGCGGCGGAAGCGATAACCGGGTTCTTCACCAAATATGGCGATGGCGGTACCGATATTAACCCGCTTCATCGCCT
TAACAAACGCCAGGGCAAACAGCTTCTCGCAGCGCTGGGCTGTCCTGAGCATCTGTATAAAAAAGTCCCCACCGCCGATT
TGGAAGACGACCGCCCTTCACTGCCTGATGAAGCCGCGCTGGGCGTAACGTATGACAATATTGACGATTACCTGGAAGGC
AAAACGCTGGACTCCGCCATCGCTAAAACTATCGAAGGCTGGTACGTAAAAACTGAACATAAGCGCCGTTTACCGATTAC
GGTATTCGACGACTTTTGGAAAAAGTAA

Upstream 100 bases:

>100_bases
GTTTTAAACACCGCTTAGCCCGCCAAACCTTGTCGTTTCAGCGGCGAAGGGTTAGCTTTAACAGGGTAAAGATTCATTTT
TTAATGTCGAAGGGGGTTAA

Downstream 100 bases:

>100_bases
GCGTACTTCTACTGTTGTGCCGGATATCGCTCGTCTATCCGGCCTCCTCTCTTCCCAGCCTCTTTTTTAATCACCCATCC
GCTGTTATACTGGATGAATA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTLQQEIIQALGAKPHINPEEEIRRSVDFLKAYLKTYPFLKSLVLGISGGQDSTLAGKLSQMAITELREETGDNALQFIA
VRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTHGVVVGT
DHAAEAITGFFTKYGDGGTDINPLHRLNKRQGKQLLAALGCPEHLYKKVPTADLEDDRPSLPDEAALGVTYDNIDDYLEG
KTLDSAIAKTIEGWYVKTEHKRRLPITVFDDFWKK

Sequences:

>Translated_275_residues
MTLQQEIIQALGAKPHINPEEEIRRSVDFLKAYLKTYPFLKSLVLGISGGQDSTLAGKLSQMAITELREETGDNALQFIA
VRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTHGVVVGT
DHAAEAITGFFTKYGDGGTDINPLHRLNKRQGKQLLAALGCPEHLYKKVPTADLEDDRPSLPDEAALGVTYDNIDDYLEG
KTLDSAIAKTIEGWYVKTEHKRRLPITVFDDFWKK
>Mature_274_residues
TLQQEIIQALGAKPHINPEEEIRRSVDFLKAYLKTYPFLKSLVLGISGGQDSTLAGKLSQMAITELREETGDNALQFIAV
RLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTHGVVVGTD
HAAEAITGFFTKYGDGGTDINPLHRLNKRQGKQLLAALGCPEHLYKKVPTADLEDDRPSLPDEAALGVTYDNIDDYLEGK
TLDSAIAKTIEGWYVKTEHKRRLPITVFDDFWKK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=275, Percent_Identity=89.8181818181818, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30476; Mature: 30345

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQQEIIQALGAKPHINPEEEIRRSVDFLKAYLKTYPFLKSLVLGISGGQDSTLAGKLS
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
QMAITELREETGDNALQFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQ
HHHHHHHHHHCCCHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
ALREAGIELSDFVRGNEKARERMKAQYSIAGMTHGVVVGTDHAAEAITGFFTKYGDGGTD
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
INPLHRLNKRQGKQLLAALGCPEHLYKKVPTADLEDDRPSLPDEAALGVTYDNIDDYLEG
CHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHCC
KTLDSAIAKTIEGWYVKTEHKRRLPITVFDDFWKK
CHHHHHHHHHHHCCEEECCCCCCCCEEEHHHHHCC
>Mature Secondary Structure 
TLQQEIIQALGAKPHINPEEEIRRSVDFLKAYLKTYPFLKSLVLGISGGQDSTLAGKLS
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
QMAITELREETGDNALQFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQ
HHHHHHHHHHCCCHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
ALREAGIELSDFVRGNEKARERMKAQYSIAGMTHGVVVGTDHAAEAITGFFTKYGDGGTD
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
INPLHRLNKRQGKQLLAALGCPEHLYKKVPTADLEDDRPSLPDEAALGVTYDNIDDYLEG
CHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHCC
KTLDSAIAKTIEGWYVKTEHKRRLPITVFDDFWKK
CHHHHHHHHHHHCCEEECCCCCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA