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The map label for this gene is hyaB [H]

Identifier: 29141562

GI number: 29141562

Start: 1171794

End: 1173587

Strand: Reverse

Name: hyaB [H]

Synonym: t1089

Alternate gene names: 29141562

Gene position: 1173587-1171794 (Counterclockwise)

Preceding gene: 29141564

Following gene: 29141561

Centisome position: 24.49

GC content: 55.46

Gene sequence:

>1794_bases
ATGAGTAACCAGTATCAAACCCAGGGTTATACCGTTAACGACGCCGGCCGTCGTCTGATCGTGGACCCGATTACCCGCAT
CGAAGGGCATATGCGCTGCGAAGTGAACATTGATGAGCAAAACGTCATTACCAATGCCGTTTCCTGCGGCACGATGTTCC
GCGGGCTGGAGATCATTCTGCAAGGCCGTGACCCACGTGACGCCTGGGCGTTTGTTGAACGTATTTGTGGTGTCTGTACC
GGGGTACATGCTCTGGCGTCGGTGTACGCTATCGAAGATGCGATTGGTATCCAGGTACCGGATAACGCCAATATTATACG
TAACATCATGCTGGCTACGTTGTGGTGCCACGATCATTTGGTCCATTTTTATCAGTTAGCCGGCATGGACTGGATTGATG
TTCTGAATGCGCTGAAAGCCGATCCACGCGCCACCTCGCAGCTGGCGCAAAGCCTTTCCGCCTGGCCGATGTCATCGCCG
GGCTACTTCTTTGACGTACAAAATCGATTGAAGAAATTTGTCGATGGCGGCCAGCTTGGGATATTCCGTAACGGCTATTG
GGGACATCCACAGTACAAACTGTCGCCAGAGGCTAATCTGATGGGTTTTGCCCACTATCTTGAAGCCCTCGACTTCCAGC
GCGAGATCGTCAAAATCCATACCATATTTGGCGGTAAGAACCCCCATCCTAACTGGATCGTCGGCGGGATGCCGTGCGCG
ATCAATCTCGATCAAAGCGGCGCGGTCGGGGCTATCAATATGGAGCGCCTCAACCTGGTGCAGTCGATCATCACCCGCAC
CGCCGACTTCATTAACAACGTGATGGTTCCGGACGCGCTGGCTATCGGCCAGTTCAATAAAGCATGGAGTCAAATTGGCA
CCGGTCTGTCGGATAAATGCGTGCTGAGCTACGGCGCATTTCCGGACATTGCCAACGACTTCAGCCAGCAAAGCCTGTTA
ATGCCGGGCGGCGCGGTGGTCAATGGCGATTTTAAAAATGTCATGCCGGTAGATTTGGCCGATCCGCAGCAGATACAGGA
GTTTGTGGATCATGCCTGGTACCGCTATCCGGACGATCGGCTGGGCCGTCATCCTTTTGACGGCATTACCGATCCCTGGT
ACAACCCGGGTGATGTCAAAGGCAGCGATACGCATATCCAGCAGCTCAACGAGCAGGAACGCTACTCCTGGATTAAAGCG
CCGCGCTGGCGGGGTCATGCCATGGAAGTCGGCCCGCTTGCCCGAACGCTTATCGCGTATCACAAAGGGGATGCCGCCAC
CATAGAGTCGGTAGATCGCATGATGTCTGCCCTTAAACTGCCGCTCTCCGGTATCCAGTCTACGCTTGGCCGTATTTTAT
GCCGCGCGCACGAAGCGCAATGGGCCGTCGGTAAGCTGCAGTATTTTTTCGACAGGCTTATGACCAACCTGAAAAACGGC
GACCTGGCCACCGCCAATACCGAGAAATGGGAACCGGCCAGTTGGCCGCAGCACTGCCGCGGCATCGGTTTTACCGAAGC
GCCTCGTGGAGCGTTGGGACACTGGGCGTCAATCCGCGATCAGAAGATTGAACTCTATCAGTGCGTAGTCCCCACCACCT
GGAACGCCAGCCCACGCGATCCTAAAAAACAGATCGGCGCCTATGAAGCGGCATTGATGGGAACGCAAATGGCGATTCCC
GACCAACCGTTAGAAATCCTGAGGACGCTGCACAGCTTCGACCCCTGCCTCGCCTGTTCAACCCACGTGCTCGGCGACGA
CGGCAGCGAACTGATTGCCGTCCAGGTACGCTAA

Upstream 100 bases:

>100_bases
TTGGCGGTCACGCTGTCGCCAGCGCGCTCAACCAACGTAAACGCCACAAACAACAGTTAGCGCAAGCCGAACAACAGCCG
GACAATGAGGATAAACAGGC

Downstream 100 bases:

>100_bases
GCGTTAAGGAAAAGAACGATGACTGGAAAGCTATCTCCACGCGTCGGGGAGGCGCGTGATACTGCCGTCAGCCACTATGT
GTTTGAAGCGCCGGTACGCC

Product: hydrogenase 1 large subunit

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MSNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCT
GVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSP
GYFFDVQNRLKKFVDGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKCVLSYGAFPDIANDFSQQSLL
MPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDRLGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKA
PRWRGHAMEVGPLARTLIAYHKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRDPKKQIGAYEAALMGTQMAIP
DQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR

Sequences:

>Translated_597_residues
MSNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCT
GVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSP
GYFFDVQNRLKKFVDGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKCVLSYGAFPDIANDFSQQSLL
MPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDRLGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKA
PRWRGHAMEVGPLARTLIAYHKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRDPKKQIGAYEAALMGTQMAIP
DQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR
>Mature_596_residues
SNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTG
VHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSPG
YFFDVQNRLKKFVDGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCAI
NLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKCVLSYGAFPDIANDFSQQSLLM
PGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDRLGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKAP
RWRGHAMEVGPLARTLIAYHKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNGD
LATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRDPKKQIGAYEAALMGTQMAIPD
QPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=597, Percent_Identity=90.9547738693467, Blast_Score=1166, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=591, Percent_Identity=42.3011844331641, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66557; Mature: 66425

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIIL
CCCCCCCCCEEECCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHEE
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHH
VHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSPGYFFDVQNRLKKFVDGGQLG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCEE
IFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCCCEE
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKC
EECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCCCE
VLSYGAFPDIANDFSQQSLLMPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDR
EEECCCCCHHHHHHHHCCEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
LGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKAPRWRGHAMEVGPLARTLIAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHH
HKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRD
CEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
PKKQIGAYEAALMGTQMAIPDQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCEECCCCCCEEEEEEC
>Mature Secondary Structure 
SNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIIL
CCCCCCCCEEECCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHEE
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHH
VHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSPGYFFDVQNRLKKFVDGGQLG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCEE
IFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCCCEE
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKC
EECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCCCE
VLSYGAFPDIANDFSQQSLLMPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDR
EEECCCCCHHHHHHHHCCEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
LGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKAPRWRGHAMEVGPLARTLIAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHH
HKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRD
CEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
PKKQIGAYEAALMGTQMAIPDQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA