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The map label for this gene is hyaD [H]

Identifier: 29141560

GI number: 29141560

Start: 1170451

End: 1171047

Strand: Reverse

Name: hyaD [H]

Synonym: t1087

Alternate gene names: 29141560

Gene position: 1171047-1170451 (Counterclockwise)

Preceding gene: 29141561

Following gene: 29141559

Centisome position: 24.44

GC content: 57.29

Gene sequence:

>597_bases
ATGAATGCGCAACGTGTAGTGGTGATGGGATTAGGAAACCTGCTGTGGGCCGATGAAGGATTCGGTATCCGGGTCGCGGA
GCGTTTGTATGCCCGATATCACTGGCCTGAAGAGGTAGAAATTGTGGACGGCGGTACGCAAGGGCTAAACCTGTTAGGTT
ATGTTGAGCAAGCCAGCCATCTCCTGCTCCTTGACGCGATCGATTACGGCCTGGCGCCGGGCAGCCTGCGAACCTATGCC
GGCGAGAAAATCCCCGCGTATCTCAGCGCCAAAAAAATGAGCCTGCATCAAAACAGTTTTTCCGAAGTTCTGGCGCTGGC
CGACATCCGCGGACATCTGCCCTGTCACATTGCGCTGGTCGGCCTGCAACCGGCGCTACTGGACGACTACGGCGGTAGCC
TGAGCGAAATAGCTCGCAGCCAGCTACCGGCGGCAGAACAGGCCGCCCTGGCGCAACTGGCCGCCTGGGGGATCGTACCG
CAGGCAAATGAGGTCGCACGCTGCCTGAACTATAAATGCCTGTCGATGGAAAATTATGAGGGAGTGCGCATACGTCAGTA
TCAGACACGTCTGGAGGGCAAAAGAAGTGGCGAATGA

Upstream 100 bases:

>100_bases
CTATCCGGGAAGACATTATGTCCGACGATACGGTGATCTCCACCATGATCAATGGCTATCGCAGCCACAAATTCCCCAAA
CCGCACGACAAGGAGCCCTC

Downstream 100 bases:

>100_bases
CACCCCGTTCTCTGCGCTATGGCAACGCCTGTTAACCCGTGGATGGCAACCGGTAGAGGCTTCCACGGTAGACGACTGGA
TCAAACGGATTGGAGATGGC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MNAQRVVVMGLGNLLWADEGFGIRVAERLYARYHWPEEVEIVDGGTQGLNLLGYVEQASHLLLLDAIDYGLAPGSLRTYA
GEKIPAYLSAKKMSLHQNSFSEVLALADIRGHLPCHIALVGLQPALLDDYGGSLSEIARSQLPAAEQAALAQLAAWGIVP
QANEVARCLNYKCLSMENYEGVRIRQYQTRLEGKRSGE

Sequences:

>Translated_198_residues
MNAQRVVVMGLGNLLWADEGFGIRVAERLYARYHWPEEVEIVDGGTQGLNLLGYVEQASHLLLLDAIDYGLAPGSLRTYA
GEKIPAYLSAKKMSLHQNSFSEVLALADIRGHLPCHIALVGLQPALLDDYGGSLSEIARSQLPAAEQAALAQLAAWGIVP
QANEVARCLNYKCLSMENYEGVRIRQYQTRLEGKRSGE
>Mature_198_residues
MNAQRVVVMGLGNLLWADEGFGIRVAERLYARYHWPEEVEIVDGGTQGLNLLGYVEQASHLLLLDAIDYGLAPGSLRTYA
GEKIPAYLSAKKMSLHQNSFSEVLALADIRGHLPCHIALVGLQPALLDDYGGSLSEIARSQLPAAEQAALAQLAAWGIVP
QANEVARCLNYKCLSMENYEGVRIRQYQTRLEGKRSGE

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=192, Percent_Identity=86.9791666666667, Blast_Score=320, Evalue=4e-89,
Organism=Escherichia coli, GI1789367, Length=155, Percent_Identity=38.0645161290323, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21709; Mature: 21709

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQRVVVMGLGNLLWADEGFGIRVAERLYARYHWPEEVEIVDGGTQGLNLLGYVEQASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCC
LLLLDAIDYGLAPGSLRTYAGEKIPAYLSAKKMSLHQNSFSEVLALADIRGHLPCHIALV
EEEEEHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GLQPALLDDYGGSLSEIARSQLPAAEQAALAQLAAWGIVPQANEVARCLNYKCLSMENYE
ECCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEECCCCCC
GVRIRQYQTRLEGKRSGE
CEEHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAQRVVVMGLGNLLWADEGFGIRVAERLYARYHWPEEVEIVDGGTQGLNLLGYVEQASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCC
LLLLDAIDYGLAPGSLRTYAGEKIPAYLSAKKMSLHQNSFSEVLALADIRGHLPCHIALV
EEEEEHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GLQPALLDDYGGSLSEIARSQLPAAEQAALAQLAAWGIVPQANEVARCLNYKCLSMENYE
ECCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEECCCCCC
GVRIRQYQTRLEGKRSGE
CEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]